More microscopy - 2.3 (1)

Question 1

What started the science of cell biology?

Answer 1

light microscopy

Question 2

Does light microscopy have limitations?

Answer 2

yes

Question 3

In which era was the electron microscope founded?

Answer 3

In the middle of the 20th century

Question 4

What revolutionised the study of cells?

Answer 4

electron microscopy

Question 5

What does electron microscopy enable biologists to see?

Answer 5

deep inside structures that were invisible under a light microscope

Question 6

What can be achieved by easily using the appropriate lenses in light microscopy?

Answer 6

increased magnification

Question 7

In light microscopy, if an image is blurred, what will not be able to be seen?

Answer 7

no more detail

Question 8

What is the limiting factor?

Answer 8

resolution

Question 9

What is electron microscopy?

Answer 9

a beam of electrons with a wavelength of less than 1nm is used to illuminate the specimen

Question 10

What can be seen in more detail with electron microscopy?

Answer 10

more detail of cell ultrastructure

Question 11

How can more detail of cell ultrastructure be seen?

Answer 11

because electrons have a much smaller wavelength than light waves

Question 12

What can electron microscopes produce images with magnifications of up to?

Answer 12

x500 000 and still have clear resolution

Question 13

What has changed the way we understand cells but still has disadvantages?

Answer 13

electron microscopes

Question 14

Are electron microscopes very expensive pieces of equipment?

Answer 14

yes

Question 15

Where can electron microscopes be used?

Answer 15

inside a carefully controlled enviroment in a dedicated space

Question 16

What can happen to specimens as a disadvantage of electron microscopy?

Answer 16

specimens can be damaged by the electron beam

Question 17

How can specimens be damaged by the electron beam?

Answer 17

because the preparation process is very complex as there is a problem with artefacts

Question 18

What are artefacts?

Answer 18

structures that are produced due to the preparation process

Question 19

How can these artefacts be eliminated?

Answer 19

through the improvement of techniques

Question 20

What are the two types of electron microscopes?

Answer 20

Transmission electron microscope (TEM) and Scanning electron microscope (SEM)

Question 21

What occurs in transmission electron microscopy?

Answer 21

a beam of electrons is transmitted through a specimen and focused to produce an image

Question 22

What occurs in scanning electron microscopy?

Answer 22

a beam of electrons is sent across the surface of a specimen and the reflected electrons are collected.

Question 23

Which type of electron microscope has a better resolution?

Answer 23

transmission electron microscope (TEM)

Question 24

What resolving power does a transmission electron microscope have?

Answer 24

0.5nm

Question 25

What resolving power does a scanning electron microscope have?

Answer 25

3-10nm

Question 26

Which electron microscope produces a stunning three-dimensional image of surfaces?

Answer 26

scanning electron microscope

Question 27

What valuable information do scanning electron microscopes give us?

Answer 27

the appearance of different organisms

Question 28

What is resolving power?

Answer 28

the smallest detail that a microscope can resolve when imaging a specimen