Molecular Techniques

Question 0

Pcr steps

Answer 0

1. Denature the copy of DNA by heat over 90degrees
   - — now you have single stranded DNa
2. Add primers and allow them to anneal to the DNA by lowering the temperature
3. Elongation by taq polymerase

Process is done within 20sec

Question 1

Pcr

Answer 1

• polymersse chain reaction
• amplify DNA (makes a bunch of copies and replicates)
• very powerful technique and can makes tons of copies quickly
• can take small pieces of DNA from a crime scene

Question 2

Restriction enzymes

Answer 2

• cut both strands of the DNA byz cutting the backbone

- ex) ecor1

Question 3

Hybridization (DNA microarrays)

Answer 3

-connecting two complementary single stranded pieces of DNA