DNA Replication

Question 0

What is the template made from

Answer 0

Dna

*youre making dna from dna

Question 1

What are you synethesizing in replication

Answer 1

Dna

Question 2

Parent dna vs daughter dna

Answer 2

Parent is what youre replicating

Question 3

Semiconservative

Answer 3

One old strand and one new strand

Question 4

Dna Helicase

Answer 4

-Unwinds DNA helix and separates strands forming the replication fork at the origin

Question 5

Single stranded binding proteins

Answer 5

Bind and stabilize the single stranded DNA

Question 6

Primase

Answer 6

• lays RNA primers on the leading and lagging strand

- has to be done for DNA pol III can work

Question 7

Dna pol III

Answer 7

• Elongates new strands complementary to the leading strand(continuously) and the lagging strand(discontinuouslsy) in the 5’-3’ direction
• the fragments on the lagging strand are called Okazaki fragments

Question 8

DNA ligase

Answer 8

• Joins the Okazaki fragments together

- seals the backbone

Question 9

Dna pol I

Answer 9

Replaces the rna primers with dna

Question 10

Topoisomerase

Answer 10

Relieves tension

Question 11

Telomerase

Answer 11

• replicates the telomeres(end of the strand)
• it extends the strand so that another primer can be layed down and elongated
• — there is a little extra genetic info but nothing is lost
• the telomeres get shorter and shorter over cell division until you eventually start to lose genetic information
• doesnt happen inprokaryotes bc there is no ends

Question 12

Dna pol I
Dna pol II
Dna pol III
Prokaryotes

Answer 12

Pol I- replaces rna primers and dna repair.
Exonuclease activity:3’-5’ and 5’-3’ exonuclease.

PolIII- elongation
Exonuclease activity: 3’-5’

Question 13

Origin of replication in prok vs euk

Answer 13

Only one spot in prok, many in euk

Question 14

Bi-direction prok and euk

Answer 14

Both are