MOLECULAR DIAGNOSIS Flashcards
1
Q
highly sensitive and specific
A
PCR
2
Q
principle of PCR Nucleic acid amplification
A
nucleic acid amplification
3
Q
PCR that involves detection of amplicons using gel amplification
A
conventional PCR
4
Q
PCR that uses fluorophores to enable detection of amplicons as they are produced
A
Real time PCR
5
Q
common target of PCR
A
Ribosomal DNA
6
Q
separation of template DNA to single strands
A
denaturation
7
Q
binding of primers specific to each target strand sequence
A
annealing
8
Q
addition of deoxyribonucleotides to produce new strands
A
extension
9
Q
temp for Denaturation
A
94 degree Celsius
10
Q
temp for annealing
A
50-65 degree Celsius
11
Q
temp for extension
A
72 degree Celsius
12
Q
extension is catalyzed by
A
Taq polymerase (Thermous aquaticus)
