MOLECULAR DIAGNOSIS

Question 1

highly sensitive and specific

Answer 1

PCR

Question 2

principle of PCR Nucleic acid amplification

Answer 2

nucleic acid amplification

Question 3

PCR that involves detection of amplicons using gel amplification

Answer 3

conventional PCR

Question 4

PCR that uses fluorophores to enable detection of amplicons as they are produced

Answer 4

Real time PCR

Question 5

common target of PCR

Answer 5

Ribosomal DNA

Question 6

separation of template DNA to single strands

Answer 6

denaturation

Question 7

binding of primers specific to each target strand sequence

Answer 7

annealing

Question 8

addition of deoxyribonucleotides to produce new strands

Answer 8

extension

Question 9

temp for Denaturation

Answer 9

94 degree Celsius

Question 10

temp for annealing

Answer 10

50-65 degree Celsius

Question 11

temp for extension

Answer 11

72 degree Celsius

Question 12

extension is catalyzed by

Answer 12

Taq polymerase (Thermous aquaticus)