Module 7 Flashcards
1
Q
- The process of transcription is going from ____ to ____.
(A) DNA, DNA
(B) DNA, RNA
(C) RNA, RNA
(D) RNA, Protein
(E) DNA, Protein
A
b
2
Q
- The main difference between prokaryotic organisms and eukaryotic organisms is that prokaryotic organisms have ___DNA while eukaryotic organisms have ___ DNA.
(A) Circular, rectangular
(B) Circular, linear
(C) Linear, circular
(D) Linear, rectangular
(E) There is no difference, both are linear
A
b
3
Q
- Supercoiling is important for DNA structure because ________.
(A) It holds together the antiparallel strands of DNA in the double helix
(B) It provides energy for transcription
(C) It condenses the DNA so that it can fit inside the cell
(D) It prevents RNA from pairing with DNA in the double helix
(E) None of the above
A
Believe c
4
Q
- Genes that encoded for polymerases, gyrases, ribosomal proteins, and other proteins essential to replication, transcription, and translation are present on ________.
(A) Chromosomes
(B) Plasmids
(C) Chromosomes and plasmids
(D) Neither chromosomes nor plasmids
(E) None of the above
A
A
5
Q
- In Bacteria, a chromosome can be distinguished from a plasmid, because a chromosome is a genetic element that ________.
(A) Is circular
(B) Is linear
(C) Encodes for essential functional genes
(D) Encodes for non-essential (“luxury”) genes
(E) None of the above
A
B
6
Q
- ________ is an essential enzyme in DNA replication that unwinds the double-stranded DNA, creating a ________ and exposing single-stranded DNA templates.
(A) DNA ligase / replication fork
(B) DNA gyrase / transcription bubble
(C) DNA helicase / replication fork
(D) DNA polymerase / transcription bubble
(E) None of the above
A
C
7
Q
- During DNA replication, the enzyme ________ synthesizes short strands of RNA (primers) that serve as a starting point for DNA elongation.
(A) Primase
(B) Polymerase
(C) Gyrase
(D) Helicase
(E) None of the above
A
A
8
Q
- Polymerase chain reaction (PCR) is a method of DNA replication in vitro that uses ______ instead of the enzyme ______ to denature double-stranded DNA and expose single-stranded DNA templates.
(A) Heat / DNA helicase
(B) Primers / DNA helicase
(C) Heat / DNA polymerase
(D) Primers / DNA polymerase
(E) None of the above
A
A
9
Q
- During the ________ step of the polymerase chain reaction (PCR), the reaction mixture is heated to 72ºC to allow the binding of the ________.
(A) Extension / DNA polymerase
(B) Extension / primers
(C) Annealing / DNA polymerase
(D) Annealing / primers
(E) None of the above
A
A
10
Q
- During the ________ step of the polymerase chain reaction (PCR), the reaction mixture is cooled to 55ºC to allow the binding of ________.
(A) Denaturing / primers
(B) Annealing / DNA polymerase
(C) Annealing / primers
(D) Extension / DNA polymerase
(E) None of the above
A
C
11
Q
- The specificity of PCR amplification is determined by which ingredient in the reaction mixture?
(A) DNA helicase
(B) DNA polymerase
(C) Nucleotides
(D) Primers
(E) None of the above
A
D
12
Q
- In the process of transcription, promoters are specific sequences of ________ that are recognized by ________.
(A) DNA / DNA polymerase
(B) RNA / DNA polymerase
(C) DNA / sigma factors
(D) RNA / ribosomes
(E) None of the above
A
B
13
Q
- ________ is an essential enzyme in DNA transcription that unwinds the double-stranded DNA, creating a ________ and exposing single-stranded DNA templates.
(A) DNA helicase / replication fork
(B) DNA helicase / transcription bubble
(C) RNA polymerase / replication fork
(D) RNA polymerase / transcription bubble
(E) None of the above
A
a
14
Q
- ________ result from hydrogen bonds that form between nucleotides in the SAME strand of an RNA molecule.
(A) Primary structures
(B) Secondary structures
(C) Tertiary structures
(D) Quaternary structures
(E) None of the above
A
b
15
Q
- The structure of RNA polymerase in Archaea is ______.
(A) More similar to Eukaryotes than to other Prokaryotes (Bacteria)
(B) More similar to other Prokaryotes (Bacteria) than to Eukaryotes
(C) Simpler (fewer subunits) than bacterial RNA polymerase
(D) Simpler (fewer subunits) than eukaryotic RNA polymerase
(E) None of the above
A
A