Module 10

Question 1

95. Essential genes in a bacterial species are found in the ______.
    (A) Proteomics
    (B) Pan Genome
    (C) Core Genome
    (D) Metagenomics
    (E) None of the above

Answer 1

C

Question 2

96. Who developed first generation sequencing?
    (A) Friedrich Miescher
    (B) Fred Sanger
    (C) James Watson
    (D) Francis Crick
    (E) Professor Erwin

Answer 2

B

Question 3

97. Which of the following links the DNA backbone?
    (A) Hydrogen bonds
    (B) Nitrogen bonds’
    (C) Phosphodiester bonds
    (D) Deoxynucleotides
    (E) None of the above

Answer 3

C

Question 4

98. Which of the following is a common term for third generation sequencing?
    (A) “Next” Generation sequencing
    (B) “Sanger” Sequencing
    (C) “Single Molecule” sequencing
    (D) “Not so hard” sequencing
    (E) None of the above

Answer 4

C

Question 5

99. SMRT and Nanopore technology are used for ___ sequencing.
    (A) Third generation
    (B) First generation
    (C) Second generation
    (D) A and B
    (E) None of the above

Answer 5

A

Question 6

100. What range are small genomes in?
     (A) 5,000,000 to 13,000,000bp
     (B) 140,000 to 1,000,000 bp
     (C) 15,000 to 120,000bp
     (D) 4-10bp
     (E) None of the above

Answer 6

B

Question 7

Probably don’t need
101. How many of the 4,300 genes in E. Coli have unknown functions?
(A) None, they all have functions
(B) 10
(C) 1,000
(D) 100
(E) 3,000

Answer 7

C

Question 8

102. The Pan Genome consists of __ genes.
     (A) Non-essential
     (B) Essential
     (C) Purple
     (D) B and C
     (E) None of the above

Answer 8

A

Question 9

103. Which sequencing method would work best for determining the sequence of a single DNA molecule?
     (A) Pyrosequencing
     (B) Third Generation Sequencing
     (C) Second generation sequencing
     (D) Chain termination sequencing
     (E) None of the above

Answer 9

B

Question 10

104. You just sequenced a genome. You have no samples to compare your genome against and there are gaps in the assembly. This is an example of
     (A) A Reference assembly of a closed genome
     (B) A De novo assembly of a closed genome
     (C) A Reference assembly of a draft genome
     (D) A De novo assembly of a draft genome
     (E) None of the above

Answer 10

d

Question 11

105. What is the main mechanism for new gene evolution?
     (A) Gene duplication
     (B) Spontaneous mutations
     (C) Natural selection
     (D) Puberty
     (E) None of the above

Answer 11

A

Question 12

106. Which of the following is NOT true for Third Generation Sequencing?
     (A) It is massively parallel
     (B) It gives you ‘Real Time’ results
     (C) It requires the amplification of DNA
     (D) Methods of Third Generation Sequencing are SMRT Technology and Nanopore technology.
     (E) None of the above

Question 13

107. Which organism is most likely to have a small genome?
     (A) Endosymbionts
     (B) Parasites
     (C) Free-living organisms
     (D) Both A and B
     (E) None of the above

Answer 13

D

Question 14

108. What is a characteristic of third generation sequencing that is different than previous two generations?
     (A) Massively parallel
     (B) Does NOT require amplification of DNA samples
     (C) High throughput
     (D) Does require amplification of DNA samples
     (E) None of the above

Answer 14

B

Question 15

109. Which physical shearing technique is the commonly used method?
     (A) Sonication
     (B) Nuclease mixes
     (C) Nebulization
     (D) UVradiation
     (E) None of the above

Answer 15

C

Question 16

110. You were given the genome of a parasite to sequence. in comparison to other genomes, how large would you expect the genome of the parasite to be?
     (A) Larger than a Human’s
     (B) Larger than a Salmon’s
     (C) Larger than a Fruit Fly’s
     (D) Larger than a Mitochondria’s
     (E) None of the Above

Answer 16

D

Question 17

111. What is the advantage of proteomics compared to transcriptomics (when identifying a specific gene)?
     (A) Proteomics identifies proteins vs. transcriptomics identifies DNA in the sample
     (B) Proteomics identifies what genes were translated vs. transcriptomics identifies all genes that were activated
     (C) Proteomics identifies all genes that were activated vs. transcriptomics identifies all genes in the sample
     (D) Proteomics identifies one specific gene vs. transcriptomics identifies all genes
     (E) None of the Above

Answer 17

B

Question 18

112. Which is NOT true of Second-Generation Sequencing?
     (A) Uses Emulsion and Bridge PCR
     (B) Requires amplification of DNA samples
     (C) Uses Fluorescent markers and laser detection
     (D) Can run massively parallel sequences together
     (E) All the above

Answer 18

C

Question 19

113. Which of the following is true about Transcriptomics?
     (A) It examines all the genetic information in an environment
     (B) It examines the translated genetic information
     (C) It examines the expressed genetic information
     (D) It takes multiple genomes from a single sequence
     (E) Two of these are correct

Answer 19

C

Question 20

114. What would be the best way to conduct a genomic study on human cancer cells?
     (A) Metagenomics
     (B) Transcriptomics
     (C) Proteomics
     (D) Genomics
     (E) All the Above

Answer 20

B

Question 21

115. Which method of sequencing would you use to determine the nucleotide composition in first generation sequencing?
     (A) Massively Parallel method
     (B) Sanger Dideoxy method
     (C) Single Molecule method
     (D) Proteomics method
     (E) None of the Above

Answer 21

B