Midterm 2 - Notes 6 (Part 4)

Question 1

In the sequence comparison between 6B and SIP1 what was being looked at?

Answer 1

Look at where is the plant protein

• what is the sub cellular of this position?
• is there some sort of function in the nucleus of cytoplasm?

Question 2

What does the 6B interacting protein 1 contain?

Answer 2

Triple helix domain

• potential DNA binding domain
• but lacks an obvious trans-activation domain

Question 3

What are they testing for with the 6B interacting protein1?

Answer 3

Subcellular localization

- transgenic plants

Question 4

How did they do their testing in 6B interacting protein1?

Answer 4

Fluorescence microscopy

Question 5

What does 6B interact with?

Answer 5

SIP1

Question 6

Where does SIP1 localize to?

Answer 6

Nucleus

Question 7

What happens to 6B if SIP1 is present?

Answer 7

It translocates into the nucleus

Question 8

What kind of domain does a SIP1 have? And not have?

Answer 8

DNA binding domain

- but no transactivation domain

Question 9

What kind of domain does 6B have? And not have?

Answer 9

Protein binding domain

- no DNA binding domain

Question 10

Can 6B act as a transactivator?

Answer 10

Yes!

Question 11

What can 6B trans-activate?

Answer 11

Transcription

- together they could act as a transcriptional regulator

Question 12

In the trans-activation targets, what was transformed?

Answer 12

Tobacco plants with 6B over-expression construct

Question 13

What was the phenotype for the plant that had the control, weak and strong groups?

Answer 13

Control = vector (normal leaves) 
Weak = 6B (slight curl of leaves)
Strong = 6B (lots of upward curling in the leaves)

Question 14

What is the upward curling of leaves caused by?

Answer 14

Additional cells at abaxial side of leaves

Question 15

What does over expression of 6B lead to?

Answer 15

Increase local cell division

- involved with the cell cycle

Question 16

What are 4 expressions of selected genes that are related to cell division/ meristem (stem cell) activity?

Answer 16

1. NTH15 = homeobox TF regulating hormone biosynthesis
2. Cyclin B = regulator of the cell cycle
3. MYBA2 = cell cycle specific TF
4. NACK1 = M phase specific kinase-like protein

Question 17

What is the normal function of SIP1 in plants?

Answer 17

Has a DNA binding domain that recognizes a cis-element known to be also the target of TFs normally activating embryonic gene expression

• expressed constitutively in adult tissues not in embryonic tissues
• has no transacting domain

Question 18

What may SIP1 be for embryonic (stem cell like) genes in adults?

Answer 18

Transcriptional repressor

Question 19

What can bacterial 6B act as?

Answer 19

A transcriptional activator

Question 20

What does bacterial 6B not have?

Answer 20

No DNA binding domain

Question 21

What can 6B bind to?

Answer 21

SIP1

Question 22

When 6B binds to SIP1 what does it activate?

Answer 22

Embryonic genes in adult cells that are normally repressed by SIP1

Question 23

Phenolics

Answer 23

They are aromatic benzene ring compounds with one or more OH groups produced by plants mainly for protecting against stress

Question 24

What are 3 examples of phenolics?

Answer 24

1. Acetosyringone
2. Coniferyl alcohol
3. Ethyl sinapate

Question 25

What do phenolics give off?

Answer 25

Wound signals

Question 26

What do anti-bacterium need to do in order to get through the bark of plants/ trees?

Answer 26

Be as close to it as it can

Question 27

How does the anti-bacterium get close to the plants?

Answer 27

It follows the wound signals

- can also go through root pores

Question 28

What are phenylpropanoid derivatives involved with?

Answer 28

Plant defence/wound sealing

Question 29

What 3 things are associated with call wall biosynthesis at wound sites?

Answer 29

1. Sugars
2. Low pH (H+)
3. Temp (< 30C)

Question 30

What is not necessary for infection?

Answer 30

Wounding

Question 31

What are 7 examples of genes that are expressed in anti-bacteria?

Answer 31

1. VirH1,2
2. VirA
3. VirB1-11
4. VirC1,2
5. VirD1-5
6. VirE1-3
7. VirG