Midterm 2 - Notes 6 (Part 4) Flashcards
In the sequence comparison between 6B and SIP1 what was being looked at?
Look at where is the plant protein
- what is the sub cellular of this position?
- is there some sort of function in the nucleus of cytoplasm?
What does the 6B interacting protein 1 contain?
Triple helix domain
- potential DNA binding domain
- but lacks an obvious trans-activation domain
What are they testing for with the 6B interacting protein1?
Subcellular localization
- transgenic plants
How did they do their testing in 6B interacting protein1?
Fluorescence microscopy
What does 6B interact with?
SIP1
Where does SIP1 localize to?
Nucleus
What happens to 6B if SIP1 is present?
It translocates into the nucleus
What kind of domain does a SIP1 have? And not have?
DNA binding domain
- but no transactivation domain
What kind of domain does 6B have? And not have?
Protein binding domain
- no DNA binding domain
Can 6B act as a transactivator?
Yes!
What can 6B trans-activate?
Transcription
- together they could act as a transcriptional regulator
In the trans-activation targets, what was transformed?
Tobacco plants with 6B over-expression construct
What was the phenotype for the plant that had the control, weak and strong groups?
Control = vector (normal leaves) Weak = 6B (slight curl of leaves) Strong = 6B (lots of upward curling in the leaves)
What is the upward curling of leaves caused by?
Additional cells at abaxial side of leaves
What does over expression of 6B lead to?
Increase local cell division
- involved with the cell cycle
What are 4 expressions of selected genes that are related to cell division/ meristem (stem cell) activity?
- NTH15 = homeobox TF regulating hormone biosynthesis
- Cyclin B = regulator of the cell cycle
- MYBA2 = cell cycle specific TF
- NACK1 = M phase specific kinase-like protein
What is the normal function of SIP1 in plants?
Has a DNA binding domain that recognizes a cis-element known to be also the target of TFs normally activating embryonic gene expression
- expressed constitutively in adult tissues not in embryonic tissues
- has no transacting domain
What may SIP1 be for embryonic (stem cell like) genes in adults?
Transcriptional repressor
What can bacterial 6B act as?
A transcriptional activator
What does bacterial 6B not have?
No DNA binding domain
What can 6B bind to?
SIP1
When 6B binds to SIP1 what does it activate?
Embryonic genes in adult cells that are normally repressed by SIP1
Phenolics
They are aromatic benzene ring compounds with one or more OH groups produced by plants mainly for protecting against stress
What are 3 examples of phenolics?
- Acetosyringone
- Coniferyl alcohol
- Ethyl sinapate
What do phenolics give off?
Wound signals
What do anti-bacterium need to do in order to get through the bark of plants/ trees?
Be as close to it as it can
How does the anti-bacterium get close to the plants?
It follows the wound signals
- can also go through root pores
What are phenylpropanoid derivatives involved with?
Plant defence/wound sealing
What 3 things are associated with call wall biosynthesis at wound sites?
- Sugars
- Low pH (H+)
- Temp (< 30C)
What is not necessary for infection?
Wounding
What are 7 examples of genes that are expressed in anti-bacteria?
- VirH1,2
- VirA
- VirB1-11
- VirC1,2
- VirD1-5
- VirE1-3
- VirG
