microscopy questions

Question 1

describe how you would use a microscope to find mean diameter of triglyceride droplets on a slide

Answer 1

1- measure with eyepiece
2- calibrate with stage micrometre
3- repeat and calculate mean

Question 2

describe how mitochondria can be separated using cell fractionation and ultracentrifugation

Answer 2

1- break open cell
2-filter to remove debris
3- use isotonic solution to prevent damage to mitochondria
4- keep cold to reduce damage to enzymes
5-centrifuge at lower speed to prevent damage
6- re spin to get mitochondria

Question 3

how does an transmission electron microscope work

Answer 3

electrons pass thru specimen
specimen must b dead
denser points appear darker as they absorb electrons

Question 4

what are the disadvantages of transmission electron microscopes

Answer 4

complex staining
only 2d image produced
specimen must b dead and thin