food tests

Question 1

what do we do to test for reducing sugars

Answer 1

1. add benedict’s reagent and heat it in a water bath

Question 2

what’s a positive colour change for reducing sugars

Answer 2

blue to brick red

Question 3

how do we test for non reducing sugars ( 2 steps)

Answer 3

use dilute hydrochloric acid and heat in the water bath

neutralise it with sodium hydrogen carbonate then do benedict’s test

Question 4

how do we test for concentration of glucose solution

Answer 4

1- line 5 test tubes on a rack
2- add 10cm 3 of 40 mM glucose solution to the test tube and 5cm 3 of distilled water to the others
3- use 5cm 3 from the first test tube and move it to the second and repeat 3 more times
4- do a benedict’s test
5- use a colorimeter and make a calibration curve with results