Microscopes - IMPORTANT Flashcards
What is the formula to calculate magnification?
Image size
Magnification x Object Size
A laser _______ ______ ______ uses ______ ______ .
A specimen is usually tagged with a ______ ______ .
The laser causes the ______ to ______ .
It can be used to look at objects at ______ _______ .
scanning confocal microscope / laser beams
fluorescent dye
dye / fluoresce
different depths
Transmission electron microscopes send beams of electrons ______ a specimin.
______ parts of the specimen ______ ______ .
They are good because they provide ______ ______ ______ however, they can only be used on ______ ______ .
through
Denser / appear darker
high-resolution images / thin samples
Electrons scanned across a specimen, knock electrons from it. These are gathered in a ______ ______ ______ .
The images produced show the ______ of the specimen and can be ______.
SEMs give ______ ______ ______ than TEMs.
cathode-ray tube
surface / 3D
lower resolution images
Define ‘resolution’.
How well a microscope distinguishes between two points that are close together.
Cytoplasms can be stained with ______ .
DNA can be stained with ______ .
Somtimes a speicimin may be ______ . Adding a stain will create ______ making the different parts show up.
eosin
methylene blue
transparent / contrast
Name the six parts of a light microscope.
Eyepiece
Objective lens
Coarse adjustment knob
Fine adjustment knob
Stage
Light
