microscope Flashcards
what occurs on the 1st century
the Romans experimented with glass.
they found the objects to appear bigger
in the 12th century, Salvino D’Armate made the
first eye glass
when and who did the 2 dutch made the first compound microscope. how did they do it
1590
zacharias jansen and his dad
by mounting 2 lenses in a tab
who developed the compound microscope with convex and concave mirror
galileo galilei
who is the author of the book micrograph
Robert Hooke
micrograph is a book that
documented wide range of observations through microscope
who achieve greater magnification of the microscope and when
anton van leeuwenheok
1674
in 1826, what did joseph jackson acheve
achromatic lense to eradicate the chromatic effect
Ernst Ruska build the…. in…
first electron microscope
1931
what is the oldest ad simplest MC
optical microscope
single lens in MC is called
loupe
what is the source of light used in simple MC
natural light
is there condenser in simple MC
no
is the magnification adjustable in simple MC
no
what is the most commonly used MC
compound MC
compound MC is also known as
biological microscope
do compound microscope uses condenser
yes
what is being used to magnify image in compound microscope
compound lens system
dissecting microscope is also known as
stereoscopic MC
stereoscopic MC is used to …
manipulate
probe
separate tissues
why do dissecting MC has an image of 3D
to observe live specimen
to perform dissectionw
what is the source of light for electron MC
electron
what MC is used in biomedical research
electron MC
what are the 2 types of electron MC
scanning electron MC
transmission electron MC
what are the similarities between TEM and SEM
they both produce a high magnified image
what are the difference between TEM and SEM
TEM - 20 000x
SEM - 20 millionx
total magnification differs
which MC appears brightly lit against dark backgrounds
dark field
which MC is usually used in platelet
phase contrast MC
name the part that supports MC
base
name the part that supports tube and connects to the base
arm
name the part that holds 2 or more objective lens and can rotate easily to change power
revolving nose system
name the part that has a horizontal platform with central opening
stage
name the part that gives controlled movement to the objects on the slides
mechanical stage
name the part that reflects light rays from source to object. one side is concave mirror while the other is a plane mirror
mirror
name the part that focuses the light
condenser
name the part that regulate the amount of light intensity passing into the condenser
diaphragm
name the part that on and off the light
power switch
name the part that holds the slides
stage clips
name the part that has a maximum and minimum of lowering stage
stage stop
name the part that moves stage left and right
stage adjustment
name the part that focus light of specimens and control the light elimination
condenser adjustment
name the part that reguates light intensity
rheostat control knob
name the part that raises or lowers the stage to bring image into focus
coarse adjustment
name the part that brings image to sharp focus
fine adjustment
what are the type of magnifying object
4x scanner objective
10x low power objective
40x high power objective
100x oil immersion objective
how to find the total magnification
lens of your eye piece x objective used
what is the power of eyepiece
10x or 15x
the eyepiece consists of…
upper eye lens and
lower field lens
what is the first vision instrument
reading stone
what AD is reading stone invented
1000AD
what is the optical instrument designed to produce magnified visual or photographic images of small objects
microscope
micrographia is he book that contains all the…
principles of the compound microscope
who is the first man to discover the cellular structure of cork
robert hooke
who invented the phase-contrast microscope
frits zernike
what kind of MC is used to study of colorless and transparent biological materials
phase-contrast microscope
what MC hat can magnify an image up to 1 million times
electron MC
what MC uses a single lens for magnification
simple MC
how many lens does compound MC have to magnify the image
objective lens
ocular lens
condenser is present in simple or compound MC?
compound
compound microscope can have up to how many objective lens
4
dissecting microscope is aka
stereoscopic microscope
stereoscope
what MC that image appears in 3D
dissecting MC
dissecting MC is used to study the… of the solid specimens
surfaces
which MC is used to count platelets
phase-contrast MC
which MC employs a special condenser
dark field MC
which MC uses for demonstration of very thin bacteria
dark field MC
dark field MC is helpful in microbiology in the identification of
spirochetes
dark field MC has a dark background created by the
special condeser
what is the source of illumination of electron MC
a beam of electrons
in electron MC, living specimen is destroyed by
the high radiation of electron beam
what kind of electron MC produces a 3D image of specimen’s surface feautures
scanning electron MC
what kind of electron MC produces a 2D image of specimens
transmission electron MC
ocular is aka
eyepiece
what are he 2 types of diaphragm
disk
iris
disk diaphragm is used in
old microscope
iris diaphragm can regulate
the amount of light by adjusting the lever
what are the 2 types of brightfield illumination
critical illumination
koehler illumination
name the type of brightfield illumination
the light source is focused at the specimen
critical illumination
name the type of brightfield illumination
the light source is focused at the condenser aperture diaphragm
koehler illumination
magnification color code for scanning
red
magnification color code for LPO
yellow
magnification color code for HPO
blue
magnification color code for OIO
white
how do we carry the MC
one under the base
other at the grasping arm
when turning on the MC and opening the diaphragm, make sure that the
light intensity dial is on the owest setting
use the what to move to center the specimen over the condenser lens
stage adjustment
use the what to move the objective as close as possible to the slide
coarse adjustment knob
when images on MC becomes clear, switch the… to make the image even sharper
fine adjustment knob
when to inspect the component parts of MC
before and after use
when not use in a long period of time, the MC should be
covered or protected from dust
do not use what to clean the MC
water
