Microbiome 8.3

Question 1

The extracellular matrix of a biofilm

contains?

Answer 1

polysaccharides, proteins, DNA that have attractive and repulsive chemical forces that hold the biofilm together and give it shape

Question 2

Biofilms release singular _____ bacteria to restart the biofilm making process

Answer 2

Planktonic

Question 3

Planktonic bacteria and biofilm bacteria have the same phenotype?

Answer 3

No

Question 4

What is a BIG problem in dental water lines?

Answer 4

biofilms

Question 5

What is a common disease associated with water untis? What the name of the bacteria?

Answer 5

Pneumonias

* Legionella pneumophila

Question 6

ORAL CAVITY has two sets of microorganisms that infect two basic domains or habitats. Name the domains?

Answer 6

salivary domain

gingival domain

Question 7

salivary domain

Answer 7

• teeth provide abundant surface area for the attachment of bacteria
• bacteria attach to surfaces of the teeth or gums via sticky polysaccharide (dextran) which are part of their biofilm
• certain bacteria can generate localized changes in oxygen and pH

Question 8

gingival domain has what type of bacteria?

Answer 8

•Many anaerobic bacteria proliferate in the deep crevices near the borders of the teeth and gums

Question 9

define dental plaque

Answer 9

biofilm of bacteria encased in polysaccharides and extracellular polymers and salivary products on the tooth

Question 10

Lactic acid producing bacteria produce
acids (like lactic acid), which lower the pH
near the tooth surface and does what

Answer 10

dissolve the calcium phosphate that makes up the tooth

Question 11

Tooth decay is caused by two?

Answer 11

streptococci!!!!

**S. sobrinus and S. mutans.

Question 12

Tooth decay is promoted by diets high in?

Answer 12

sucrose

Question 13

Sucrose is fermented to lactic acid by _____ within the plaque

Answer 13

S. mutans

Question 14

•Lactic acid reduces ____ of tooth? this causes?

Answer 14

reduces pH of plaque causing demineralization of tooth at plaque

Question 15

dental biofilm is a mixed biofilm of many bacterial and ____ species

Answer 15

fungal

Question 16

Commensal Streptococci, such as

Streptococcus salivarius controls what bacterias participate in biofilm formation? HOW?

Answer 16

controls S. mutans participation in biofilm formation through its production of FRUCTANASE

Question 17

Probiotic

Answer 17

Live organism that when ingested is believed to provide benefit to the host

Question 18

Prebiotic

Answer 18

Food ingredient that supports the growth of one or more members of the microbiota

Question 19

Do probiotics work?

Answer 19

they might work, but only temporary (they re competing by whats already there). So you’ll have to keep taking the pill. Also, need to feed it for them to survive, so pre-biotics is a good idea

Question 20

Can probiotics colonize your intestine for any amount of time?

Answer 20

Yes, but only for 1-2 weeks

Question 21

what is one thing to remember about pro-biotics?

Answer 21

you must feed these bacteria that are in the probiotic mix and they must compete with existing microbiome established throughout your life

Question 22

Purpose of pro-biotics?

Answer 22

thought to be a beneficial mixture of microbes