Bacterial Structure 2&3. 3 Flashcards
Cell wall of bacteria
a structure exterior to the cell membrane that provides rigidity and stability to the bacterial cell
- Cell walls consist of Peptidoglycan
- G- or G+
Gram (+) composed of?
peptidoglycan and teichoic acid (purple)
Gram (-) composed of?
peptidoglycan and an outer membrane (red)
Peptidoglycan consists of?
strands of alternating repeats of N-acetyl glucose-like sugars
•N-acetylglucosamine (NAG)
•N-acetylmuramic acid (NAM)
N-acetylmuramic acid (NAM) is cross linked between?
strands by short peptides
Our body prodcuces what enzyme that destroys peptidoglycan? This leads to?
Lysozyme
*leads to lysis of bacteria cell
NAG and NAM are linked by?
a beta 1-4 glycosidic covalent bond -this is the lysozyme sensitive region
What four AA make the tetrapeptide bound to NAM in peptidoglycan?
- L-alanine
- D-alanine
- D-glutamic acid
- either lysine or diaminopimelic acid
Tetrapeptide cross-links are formed by? Provide the cell wall with what physical traits?
Tetrapeptides interaction on separate strands of peptidoglycan; provide the cell wall of prokaryotes with extreme strength and rigidity
define peptidoglycan interbridge
Tetrapeptide cross-links between layers of peptidoglycan
In G+, peptidoglycan interbridge is often?
a pentaglycine
In G-, peptidoglycan interbridge is often?
a direct link between one tetrapeptide that often contains a diaminopimelic acid (DAP) and the other tetrapeptide
Penicillin preevnts?
prevents crosslinkage of cell wall peptidoglycan (weakening the cell wall) aka transpeptidation
A hydrophobic alcohol called _______ facilitates transport of new glycan units across cytoplasmic membrane for formation of peptidoglycan
bactoprenol
bactoprenol and vancomycin inhibit?
the bactoprenal dependent process of transporting glycan units across cytoplasm