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Medical Microbiology II - 4336 > Microbiology review powerpoint > Flashcards

Microbiology review powerpoint Flashcards

1
Q

Subcutaneous fungi

A

Chromoblastomycosis, Sporotrichosis, Mycetoma (eumycotic), Phaeohyphomycosis

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2
Q

Opportunistic fungi

A

Aspergillosis, Candidosis, Cryptococcosis, Geotrichosis, Mucormycosis, Fusariosis, Trichosporonosis, and others

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3
Q

Systemic fungi

A

Aspergillosis, blastomycosis, candidosis, coccidioidomycosis, Adiaspiromycosis, Emmonsiosis, Histoplasmosis, Cryptocococcos, Geotrichosis, Paracoccidioidomycosis, Mucormycosis, Fusariosis, Trichosporonosis

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4
Q

Samples

CSF blank
Blood blank
eye scrapping blank

A

1.)CSF (should never be refrigerated),
2.)Blood (lysis centrifugation system to recover dimorphic fungi),
3.)Eye scrapping (media without cycloheximide),

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5
Q

Hair skin and nail scrapping blank

Vignial samples blank

time of urine not accepted

Other specimen types

Mycosel agar is satisfied for

Add Olive or olive oil for

A

1.)Hair, skin and nail scrapping (Mycosel agar is satisfactory for dermatophytes, add olive oil or olive oil saturated paper to recover M. furfur)
2.)Vaginal samples
3.)Urine: 24-hr urine is not accepted.
4.)Tissue, bone marrow and sterile body

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6
Q

Saprobic and pathogenic fungi agar

A

BHI, Potato flake agar (PFA), Sabouraud dextrose with BHI

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7
Q

Yeast and filamentous fungi agar

A

Chromogenic agar

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8
Q

Dermatophytes agar

A

Mycosel agar, BHI with antibiotics, Inhibitory mold agar (IMA), Dermatophyte test medium (DTM), Yeast-extract phosphate agar

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9
Q

BHI biphasic media

A

fungi from blood

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10
Q

Differential media

AA agar

A

Acetate ascospore)

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11
Q

Differential media

Christensen urea agar

A

Cryptococcus, Trichosporon

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12
Q

Cornmeal agar with tween 80 and trypan blue

Differential media

A

Chlamydospore from C. albicans

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13
Q

Cottonseed conversion agar

Differential media

A

Dimorphic fungi

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14
Q

Niger seed agar (Birdseed agar)

Differential media

A

Cryptococcus

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15
Q

Potato dextrose agar

Differential media

A

The pigment produced by T.rubrum

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16
Q

urea agar

Differential media

Urea positive fungi

A

Cryptococcus, T. mentagrophytes and Trichosporon. Urease negative: T. rubrum

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17
Q

Direct microscopic detection of fungi

A

KOH preparation
Saline wet mount: Fungal elements
Calcofluor white stain
India ink stain: Cryptococcus in CSF

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18
Q

Direct microscopic detection of fungi

blank cotton or blank blue what

A

Lactophenol cotton or aniline blue wet mount

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19
Q

Direct microscopic detection of fungi

Masson Fontana stain

A

Masson-Fontana stain: melanin pigment in fungal cell wall

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20
Q

Direct microscopic detection of fungi

Methenamine silver stain

A

Methanamine silver stain: Fungi in tissue

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21
Q

Direct microscopic detection of fungi

PAS stain

A

Periodic acid Schiff (PAS) stain

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22
Q

Yeast like fungi

A

Histoplasma capsulatum, Sporothrix spp., cryptococcus spp. M furfur, blasomyces spp, Paracoccidioides brasiliensis.

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23
Q

Sherules

A

: Coccidioides spp

24
Q

Yeast and pseudohyphae

Yeast and hyphae

A

Candida spp. (except C. glabrata)
Yeast and hyphae: M. furfur

25
Q

Paucispetate hyphae

A

Mucor, Rhizopus and other genera

Broad ribbon ribbon like hyphae

26
Q

Hyaline septate hyphae

A

Dermatophytes, Aspergillus, Geotrichum spp., trichosporon spp.

27
Q

Dematiaceous septate hyphae

A

Bipolaris, Cladosporium, curvularia, exophiala, hortaea, phialophora

28
Q

Scelortic bodies

A

(thick-walled cells with transverse septations, round, brown): Cladosporium carrionii, Fonsecaea compacta, F. pefrosoi, Phialophora verrucose, Rhicocladiella aquaspersa

29
Q

Granules

A

Acremonium, Aspergillus nidulans, curvularia, Exophiala, Fusarium, Madurella etc

30
Q

Foot cell

A

Is where spores develop

31
Q

Clamydospore in the class description

A

Septum becomes thick

32
Q

Cryptococcus have

33
Q

Exo antigen tests

A

Like an immunodiffusion test

Detects fungal antigen

Need to know antigen to find antibody

or

Need to know antibody to find antigen

34
Q

Tease specimen to

A

Spread out specimen

35
Q

If fungus produces spores then place inside class

A

2 safety cabinet

36
Q

Aspigillus fumigates have what toxin

A

Elastase to break down elastin

37
Q

Parricoccidoises release

38
Q

Arthroconnidia

A

Separate septum from hyphae

39
Q

37C

A

Creamy white colony

40
Q

25C

A

Different colony morphology

41
Q

Blastomyces stain and come from blood to peripheral what and deposit into what

A

Red

Circulation

Skin

42
Q

Histoplasma have what zone

43
Q

Histoplasma stains

A

PAS and Gomori stain

44
Q

Yeast may be what

45
Q

How to differentiate fungus

A

Based on temperature or incubate

46
Q

Morphology of fungus depends on

A

Temperature

47
Q

Biseriate have

A

Metulae= clear out layer

48
Q

Macroslamidya and Microclamidya are not apart of

49
Q

Ascocarp

A

Area that surrounds spores

50
Q

Ascospores are

51
Q

Coccidiosis species are

A

Groups of yeast

52
Q

Masson stain is a

A

Cutaneous fungus stain

53
Q

KOH dissolves what

A

Tissue samples

54
Q

After KOH go to

55
Q

T rubrum

A

urease negative

56
Q

T mentographytes

A

urease positive

57
Q

Fungus can be inside what a lot

Medical Microbiology II - 4336 (20 decks)

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