Microbiology review powerpoint

Question 1

Subcutaneous fungi

Answer 1

Chromoblastomycosis, Sporotrichosis, Mycetoma (eumycotic), Phaeohyphomycosis

Question 2

Opportunistic fungi

Answer 2

Aspergillosis, Candidosis, Cryptococcosis, Geotrichosis, Mucormycosis, Fusariosis, Trichosporonosis, and others

Question 3

Systemic fungi

Answer 3

Aspergillosis, blastomycosis, candidosis, coccidioidomycosis, Adiaspiromycosis, Emmonsiosis, Histoplasmosis, Cryptocococcos, Geotrichosis, Paracoccidioidomycosis, Mucormycosis, Fusariosis, Trichosporonosis

Question 4

Samples

CSF blank
Blood blank
eye scrapping blank

Answer 4

1.)CSF (should never be refrigerated),
2.)Blood (lysis centrifugation system to recover dimorphic fungi),
3.)Eye scrapping (media without cycloheximide),

Question 5

Hair skin and nail scrapping blank

Vignial samples blank

time of urine not accepted

Other specimen types

Mycosel agar is satisfied for

Add Olive or olive oil for

Answer 5

1.)Hair, skin and nail scrapping (Mycosel agar is satisfactory for dermatophytes, add olive oil or olive oil saturated paper to recover M. furfur)
2.)Vaginal samples
3.)Urine: 24-hr urine is not accepted.
4.)Tissue, bone marrow and sterile body

Question 6

Saprobic and pathogenic fungi agar

Answer 6

BHI, Potato flake agar (PFA), Sabouraud dextrose with BHI

Question 7

Yeast and filamentous fungi agar

Answer 7

Chromogenic agar

Question 8

Dermatophytes agar

Answer 8

Mycosel agar, BHI with antibiotics, Inhibitory mold agar (IMA), Dermatophyte test medium (DTM), Yeast-extract phosphate agar

Question 9

BHI biphasic media

Answer 9

fungi from blood

Question 10

Differential media

AA agar

Answer 10

Acetate ascospore)

Question 11

Differential media

Christensen urea agar

Answer 11

Cryptococcus, Trichosporon

Question 12

Cornmeal agar with tween 80 and trypan blue

Differential media

Answer 12

Chlamydospore from C. albicans

Question 13

Cottonseed conversion agar

Differential media

Answer 13

Dimorphic fungi

Question 14

Niger seed agar (Birdseed agar)

Differential media

Answer 14

Cryptococcus

Question 15

Potato dextrose agar

Differential media

Answer 15

The pigment produced by T.rubrum

Question 16

urea agar

Differential media

Urea positive fungi

Answer 16

Cryptococcus, T. mentagrophytes and Trichosporon. Urease negative: T. rubrum

Question 17

Direct microscopic detection of fungi

Answer 17

KOH preparation
Saline wet mount: Fungal elements
Calcofluor white stain
India ink stain: Cryptococcus in CSF

Question 18

Direct microscopic detection of fungi

blank cotton or blank blue what

Answer 18

Lactophenol cotton or aniline blue wet mount

Question 19

Direct microscopic detection of fungi

Masson Fontana stain

Answer 19

Masson-Fontana stain: melanin pigment in fungal cell wall

Question 20

Direct microscopic detection of fungi

Methenamine silver stain

Answer 20

Methanamine silver stain: Fungi in tissue

Question 21

Direct microscopic detection of fungi

PAS stain

Answer 21

Periodic acid Schiff (PAS) stain

Question 22

Yeast like fungi

Answer 22

Histoplasma capsulatum, Sporothrix spp., cryptococcus spp. M furfur, blasomyces spp, Paracoccidioides brasiliensis.

Question 23

Sherules

Answer 23

: Coccidioides spp

Question 24

Yeast and pseudohyphae

Yeast and hyphae

Answer 24

Candida spp. (except C. glabrata)
Yeast and hyphae: M. furfur

Question 25

Paucispetate hyphae

Answer 25

Mucor, Rhizopus and other genera Broad ribbon ribbon like hyphae

Question 26

Hyaline septate hyphae

Answer 26

Dermatophytes, Aspergillus, Geotrichum spp., trichosporon spp.

Question 27

Dematiaceous septate hyphae

Answer 27

Bipolaris, Cladosporium, curvularia, exophiala, hortaea, phialophora

Question 28

Scelortic bodies

Answer 28

(thick-walled cells with transverse septations, round, brown): Cladosporium carrionii, Fonsecaea compacta, F. pefrosoi, Phialophora verrucose, Rhicocladiella aquaspersa

Question 29

Granules

Answer 29

Acremonium, Aspergillus nidulans, curvularia, Exophiala, Fusarium, Madurella etc

Question 30

Foot cell

Answer 30

Is where spores develop

Question 31

Clamydospore in the class description

Answer 31

Septum becomes thick

Question 32

Cryptococcus have

Answer 32

Capsules

Question 33

Exo antigen tests

Answer 33

Like an immunodiffusion test Detects fungal antigen Need to know antigen to find antibody or Need to know antibody to find antigen

Question 34

Tease specimen to

Answer 34

Spread out specimen

Question 35

If fungus produces spores then place inside class

Answer 35

2 safety cabinet

Question 36

Aspigillus fumigates have what toxin

Answer 36

Elastase to break down elastin

Question 37

Parricoccidoises release

Answer 37

Proteases

Question 38

Arthroconnidia

Answer 38

Separate septum from hyphae

Question 39

37C

Answer 39

Creamy white colony

Question 40

25C

Answer 40

Different colony morphology

Question 41

Blastomyces stain and come from blood to peripheral what and deposit into what

Answer 41

Red Circulation Skin

Question 42

Histoplasma have what zone

Answer 42

Clear

Question 43

Histoplasma stains

Answer 43

PAS and Gomori stain

Question 44

Yeast may be what

Answer 44

Acid fast

Question 45

How to differentiate fungus

Answer 45

Based on temperature or incubate

Question 46

Morphology of fungus depends on

Answer 46

Temperature

Question 47

Biseriate have

Answer 47

Metulae= clear out layer

Question 48

Macroslamidya and Microclamidya are not apart of

Answer 48

Hypae

Question 49

Ascocarp

Answer 49

Area that surrounds spores

Question 50

Ascospores are

Answer 50

Sexual

Question 51

Coccidiosis species are

Answer 51

Groups of yeast

Question 52

Masson stain is a

Answer 52

Cutaneous fungus stain

Question 53

KOH dissolves what

Answer 53

Tissue samples

Question 54

After KOH go to

Answer 54

wet mount

Question 55

T rubrum

Answer 55

urease negative

Question 56

T mentographytes

Answer 56

urease positive

Question 57

Fungus can be inside what a lot

Answer 57

WBCs