Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Medical Microbiology II - 4336 > LAB EXAM 1 > Flashcards

LAB EXAM 1 Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards USMLE® Step 1 flashcards
1
Q

What are the reagents used for acid-fast staining?

A

-Kinyoun carbol-fuchsin
-acid alcohol
-Methylene blue counterstain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Why are mycobacteria difficult to stain?

A

-lipophilic
-cell-wall-bound mycolic acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is the reverse CAMP test used to differentiate?

A

Clostridium perfringens from other Clostridium sp.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Reverse CAMP test:

Colostridium perfringens is streaked at right angles to _______________

A

Streptococcus agalactiae (group B)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what is the E-test used to determine?

A

the MIC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is needed to perform the E-test?

A

-Muller Hinton Agar
-bacterial suspension
-0.5 McFarland standard
-E-test strip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Two spore-forming anaerobes?

A

-Clostridium perfringes and botulism and Teni
-bacillus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

a genus of Gram-negative, obligate anaerobic bacteria, non-endospore-forming bacilli

A

Bacteroides

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is the morphology of Clostridium tetani?

A

drumstick or tennis racket GPR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Gram stain shows “box car” shaped spores

A

Clostridium perfringens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Clostridium ___________ shows a double zone of hemolysis

A

perfringens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Morphology of fusobacterium?

A

gram stain will look slender, spindle shape GNR with tapered ends

To make one end gradually smaller then the other

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

examples of three types of mircosporum?

A

-Mircosporum canis
-Mircosporum gyseum
-Mircosporum nanum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Macroconidium vs Microcondiida?

A

Microconidia: Small, unicellular conidia that are round, elliptical, or pyriform (pear-shaped).
Macroconidia: Large, multi-septate conidia that are club- or spindle-shaped

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q
A

Fusarium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q
A

Bipolaris

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q
A

Mucor

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q
A

Histoplasma capsulatam

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q
A

Blastomyces dermatitidis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q
A

Blastomyces dermatitidis

(look for broad base)

21
Q

What aspergillus species appears green macroscopically?

What appears blue

A

Aspergillus fumigatus

-Aspergillus flavus (more of a bluish appearance?)

22
Q
A

Alternaria

23
Q
A

Trichophyton (mentagrophytes) and rubrum

24
Q
A

Microsporum canis

25
Q
A

Microsporum gyseum

26
Q
A

Microsporum nanum

27
Q
A

B. dermatitidis

28
Q
A

Coccidioides immitis

29
Q
A

Coccidioides spherule (spherule containing endospores)

30
Q
A

Paracoccidioides brasiliensis (wheel like structure)

31
Q

Direct detection?

A

-Calcofluor white
-KOH
-Histology stains

32
Q

An asexual conidium that forms by a blowing out or budding process

A

Blastoconidia

33
Q

What are structures seen in yeast?

What is most common in Candida albicans

A

-Blastoconidia
-Chlamydospores and pseudohyphae are most common in Candida albicans

34
Q

Buds elongate, fail to dissociate, and form subsequent buds – they have cell wall constrictions rather than true septa

A

Pseudohyphae

35
Q
A

candida

36
Q

What does MBC stand for?

A

Minimum bactericidal concentration- lowest concentration of antibacterial agent required to kill a bacteria.

37
Q

What does MIC stand for?

A

Minimum inhibitory concentration- lowest concentration of an antibiotic at which bacterial growth is completly inhibited

38
Q
A

-

39
Q

Anaerobic growth chamber indicators:

The pink tablet will turn what color if anaerobic?

A

remain pink

40
Q

Anaerobic growth chamber indicators:

Blue strip will change to _______ if anaerobic conditions are maintained.

A

white

41
Q

a physical property that gives a bacterium the ability to resist decolorization by acids during staining procedures. This means that once the bacterium is stained, it cannot be decolorized using acids routinely used in the process.

A

Acid fastness

42
Q

How is Mucor, Rhizopus, and Absidia differentiated?

A

-Mucor: without root
-Rhizopus: with root
-Absidia: roots are not directly under sporangiophore (alternating in between)

43
Q

four dermatophytes…

A

canis, gypseum, nanum, and Trichophyton mentagrophytes

44
Q

no root…

A

mucor

45
Q

root directly underneath sporangiophore

A

rhizopus

46
Q

roots are not directly under sporangiophore (alternating in between)

A

Absidia

47
Q

What are two examples of acid fast bacteria?

A

-Mycobacterium tuberculosis
-Nocardia (weak)

48
Q

Acid fast positive

A

Pink or red

49
Q

Acid fast negative

A

Blue or green

Medical Microbiology II - 4336 (20 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions