Microbiology Lab Final

Question 1

What are the Gram Positive Bacteria Used during lab?

Answer 1

• S. aureus
• S. epidermidis
• M. luteus
• E. faecalis

Question 2

What are the Gram Negative Bacteria Used during lab?

Answer 2

• B. subtilis
• P. aeruginosa
• E. aerogenes
• E. coli
• K. pneumoniae

Question 3

Causes endophthalmitis, right sided endocarditis, and meningitis in comporomised patients.

Answer 3

B. subtilis

Question 4

Part of the normal flora of the human intestine; some strains cause diarrheal disease or urinary tract infections.

Answer 4

E. coli

Question 5

Part of the normal microbiota of human skin. Causative agent of skin and wound infections, internal infections, toxic shock syndrome, scaled skin syndrome, and food poisoning.

Answer 5

S. aureus

Question 6

Found in nasty water. Causes digestion issues and diarrhea.

Answer 6

Giardia lamblia

Question 7

Causes Malaria. Transmitted by anopheles mosquitos.

Answer 7

Plasmodium

Question 8

Causes toxoplasmosis in humans. Primary host for parasite is domestic cats.

Answer 8

Toxoplasma gondii

Question 9

Causes abscess in liver.

Answer 9

Entamoeba histolytica

Question 10

Tape worm

Answer 10

Taenia saginata

Question 11

Common liver fluke

Answer 11

Fasciola hepatica

Question 12

Pin worm

Answer 12

Enterubis vermicularis

Question 13

Spherical bacteria

Answer 13

Cocci

Question 14

Rod shaped bacteria

Answer 14

Bacilli

Question 15

Curved rod bacteria

Answer 15

Vibrios

Question 16

Corkscrew shaped bacteria

Answer 16

Spirilla

Question 17

Flexible helices.

Answer 17

Spirochetes

Question 18

Pairs

Answer 18

Diplococci/diplobacilli

Question 19

Chains

Answer 19

Streptococci/Streptobacilli

Question 20

Grape like clusters

Answer 20

Staphlyococci/staphlybacilli

Question 21

What are the general growth mediums used in the lab?

Answer 21

• TSB (trypticase soy broth)

- TSA (trypticase soy agar)

Question 22

What are the 4 steps in a gram stain?

Answer 22

• Crystal Violet
• Iodine
• Decolorizer
• Safranin

Question 23

What is the function of crystal violet in a gram stain?

Answer 23

Stains peptidoglycan

Question 24

What is the function of Iodine in a gram stain?

Answer 24

Mordant, fixes purple color in place

Question 25

What is the function of decolorizer in a gram stain?

Answer 25

Pulls purple from sample. (Too much can cause a gram positive to appear gram negative)

Question 26

What is the function of safranin in a gram stain?

Answer 26

Works as a counter stain

Question 27

What is the difference in a gram positive and gram negative cell wall?

Answer 27

Gram negative has a thin layer of peptidoglycan while gram positive has a thick layer of peptidoglycan.

Question 28

What can you tell by just looking at a bacteria through a microscope?

Answer 28

- Gram positive or negative. - Morphology - Colony

Question 29

An organism, such as a bacterium, that can live only in the absence of oxygen.

Answer 29

Obligate anaerobe

Question 30

An organism, such as a bacterium, that can live only in the presence of oxygen.

Answer 30

Obligate aerobe

Question 31

surviving and growing in small amounts of air

Answer 31

Aerotolerant

Question 32

An organism, such as a bacterium, that can live in the absence as well as in the presence of atmospheric oxygen.

Answer 32

Facultative anaerobe

Question 33

What is the difference in Eukaryotic and Prokaryotic organisms?

Answer 33

Eukarytoic is US (humans). Have membrane bound organelles. Prokayrotic do not have membrane bound organelles. Ribosomes exsist in both. 80s ribosomes in eukarytoes and 70s in prokarytoes.

Question 34

What is a protein synthesis inhibitor?

Answer 34

Azithromycin (Z-pak)

Question 35

Prevents the cross linking of peptidoglycan?

Answer 35

Penicillins

Question 36

DNA synthesis inhibitors?

Answer 36

Cipro

Question 37

What are 3 methods for counting bacteria?

Answer 37

- Filtration - Serial dilution - Most probable number

Question 38

What is the magnification of a microscope that is using a 10x lens with a 30x lens.

Answer 38

300x

Question 39

Amplifies DNA

Answer 39

PCR (Polymerase chain reaction)

Question 40

What are the 3 parts of a virus?

Answer 40

- Protein capsid - Nucleic acid - Glycoprotein