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Microbiology > Microbiology Lab Final > Flashcards

Microbiology Lab Final Flashcards

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Biology 101 flashcards
1
Q

What are the Gram Positive Bacteria Used during lab?

A
  • S. aureus
  • S. epidermidis
  • M. luteus
  • E. faecalis
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2
Q

What are the Gram Negative Bacteria Used during lab?

A
  • B. subtilis
  • P. aeruginosa
  • E. aerogenes
  • E. coli
  • K. pneumoniae
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3
Q

Causes endophthalmitis, right sided endocarditis, and meningitis in comporomised patients.

A

B. subtilis

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4
Q

Part of the normal flora of the human intestine; some strains cause diarrheal disease or urinary tract infections.

A

E. coli

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5
Q

Part of the normal microbiota of human skin. Causative agent of skin and wound infections, internal infections, toxic shock syndrome, scaled skin syndrome, and food poisoning.

A

S. aureus

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6
Q

Found in nasty water. Causes digestion issues and diarrhea.

A

Giardia lamblia

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7
Q

Causes Malaria. Transmitted by anopheles mosquitos.

A

Plasmodium

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8
Q

Causes toxoplasmosis in humans. Primary host for parasite is domestic cats.

A

Toxoplasma gondii

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9
Q

Causes abscess in liver.

A

Entamoeba histolytica

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10
Q

Tape worm

A

Taenia saginata

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11
Q

Common liver fluke

A

Fasciola hepatica

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12
Q

Pin worm

A

Enterubis vermicularis

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13
Q

Spherical bacteria

A

Cocci

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14
Q

Rod shaped bacteria

A

Bacilli

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15
Q

Curved rod bacteria

A

Vibrios

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16
Q

Corkscrew shaped bacteria

A

Spirilla

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17
Q

Flexible helices.

A

Spirochetes

18
Q

Pairs

A

Diplococci/diplobacilli

19
Q

Chains

A

Streptococci/Streptobacilli

20
Q

Grape like clusters

A

Staphlyococci/staphlybacilli

21
Q

What are the general growth mediums used in the lab?

A
  • TSB (trypticase soy broth)

- TSA (trypticase soy agar)

22
Q

What are the 4 steps in a gram stain?

A
  • Crystal Violet
  • Iodine
  • Decolorizer
  • Safranin
23
Q

What is the function of crystal violet in a gram stain?

A

Stains peptidoglycan

24
Q

What is the function of Iodine in a gram stain?

A

Mordant, fixes purple color in place

25
Q

What is the function of decolorizer in a gram stain?

A

Pulls purple from sample. (Too much can cause a gram positive to appear gram negative)

26
Q

What is the function of safranin in a gram stain?

A

Works as a counter stain

27
Q

What is the difference in a gram positive and gram negative cell wall?

A

Gram negative has a thin layer of peptidoglycan while gram positive has a thick layer of peptidoglycan.

28
Q

What can you tell by just looking at a bacteria through a microscope?

A
  • Gram positive or negative.
  • Morphology
  • Colony
29
Q

An organism, such as a bacterium, that can live only in the absence of oxygen.

A

Obligate anaerobe

30
Q

An organism, such as a bacterium, that can live only in the presence of oxygen.

A

Obligate aerobe

31
Q

surviving and growing in small amounts of air

A

Aerotolerant

32
Q

An organism, such as a bacterium, that can live in the absence as well as in the presence of atmospheric oxygen.

A

Facultative anaerobe

33
Q

What is the difference in Eukaryotic and Prokaryotic organisms?

A

Eukarytoic is US (humans). Have membrane bound organelles.
Prokayrotic do not have membrane bound organelles.
Ribosomes exsist in both. 80s ribosomes in eukarytoes and 70s in prokarytoes.

34
Q

What is a protein synthesis inhibitor?

A

Azithromycin (Z-pak)

35
Q

Prevents the cross linking of peptidoglycan?

A

Penicillins

36
Q

DNA synthesis inhibitors?

A

Cipro

37
Q

What are 3 methods for counting bacteria?

A
  • Filtration
  • Serial dilution
  • Most probable number
38
Q

What is the magnification of a microscope that is using a 10x lens with a 30x lens.

A

300x

39
Q

Amplifies DNA

A

PCR (Polymerase chain reaction)

40
Q

What are the 3 parts of a virus?

A
  • Protein capsid
  • Nucleic acid
  • Glycoprotein

Microbiology (13 decks)

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