Microbio

Question 1

Gram-stain?

Answer 1

differentiate MOST bacteria

Question 2

Ziehl-Neelsen stain?

Answer 2

differentiate between MYCOBACTERIA + other bacteria

Question 3

Catalase test?

Answer 3

differentiate between STAPH + STREP

(add H2O2)

Question 4

Coagulase test?

Answer 4

differentiate between S.AUREUS + other staph

(S.aureus produces coagulase to convert soluble fibrinogen to insoluble fibrin)

Question 5

Haemolysis test / blood agar?

Answer 5

differentiate between STREP PNEUMONIA/VIRIDANS + STREP PYOGENES/S.AUREUS

Question 6

Optochin test?

Answer 6

differentiate between STREP PNEUMONIA + VIRIDANS

Question 7

Oxidase test?

Answer 7

differentiate between bacteria with + without CYTOCHROME OXIDASE

Question 8

MacConkey agar?

Answer 8

differentiate between GRAM -ve bacteria that can + can’t FERMENT LACTOSE

Question 9

XLD agar?

Answer 9

used to differentiate SALMONELLA + SHIGELLA

Question 10

CLED agar?

Answer 10

used to grow + identify URINARY TRACT pathogens + prevent PROTEUS SWARMING

Question 11

Sabourard agar?

Answer 11

used to grow FUNGI + inhibits bacterial growth

Question 12

Lowenstein-Jensen medium?

Answer 12

used to grow MYCOBACTERIA