Media Inoculation

Question 1

Colony

Answer 1

a visible mass of microbial cells arising from one cell or from a group of the same microbes

Question 2

Pure culture

Answer 2

a single species present in the medium

Question 3

Streak plating

Answer 3

a method of isolating a culture by spreading microorganisms over the surface of a solid culture medium

Question 4

Forms of growth media

Answer 4

• Broth
• Slants
• Deeps
• Plates

Question 5

Broth

Answer 5

• Used to grow large numbers quickly
• Use sterile loop to transfer sample from broth culture into sterile broth

Question 6

Slant

Answer 6

• used for isolating and determining colony morphology but conserves material and space
• use sterile loop position loop at base of slant surface; zig-zag upward over surface

Question 7

Deeps

Answer 7

• may be solid or semi-solid; used to determine O2 requirements and motility
• Use sterile needle
• Perform stab in the center of the deep and extend Downward almost to the bottom withdraw needle from same pathway

Question 8

Plates

Answer 8

• Used for isolating and determining colony morphology
• Perform the streak technique
• Be sure to sterilize the loop between each streak

Question 9

Distinguish between a pure culture and a mixed/contaminated culture.

Answer 9

one species vs. a variety

Question 10

Recognize the quadrant streak method

Answer 10

Be able to determine whether isolation was achieved