Basic Microscopy

Question 1

What microscope is used in the microbiology lab

Answer 1

compound light microscope

Question 2

Magnification of the ocular lens (eyepiece)

Answer 2

10x

Question 3

Magnification of the scanning lens (red)

Answer 3

4x

total 40x

Question 4

Magnification of the low power lens (yellow)

Answer 4

10x

total 100x

Question 5

Magnification of the high power lens (blue/high dry)

Answer 5

40x

total 400x

Question 6

Magnification of the Oil Immersion lens (white)

Answer 6

100x

total 1000x

Question 7

_______ _________: supports objective lenses and rotates them into position

Answer 7

revolving nose piece

Question 8

______: holds slide

Answer 8

stage

Question 9

_______ ______ _______: slide holder and knobs that position slide

Answer 9

mechanical stage control

Question 10

_____ ______ opening in stage that allows light to illuminate

Answer 10

stage aperture

Question 11

__________: controls the amount of light entering stage aperture

Answer 11

diaphragm

Question 12

_________ concentrates light source into a cone of light that passes through diaphragm

Answer 12

condenser

Question 13

______-_________ _____: brings speciment into focus quicly UP AND DOWN

Answer 13

course adjustment knob

Question 14

______ _________ ______: slowly brings speciment into sharper focus

Answer 14

Fine adjustment knob

Question 15

_____: supports coarse and fine adjustment knobs, holds head and stage

Answer 15

Arm

Question 16

______: holds oculars and houses mirrors

Answer 16

head

Question 17

_____ houses light source, supports and stabilizes entire microscope

Answer 17

base

Question 18

_______ ___________: use visible light as a source of illumination

Answer 18

light microscope

Question 19

________ ________: contains more than one magnifying lens. TOTAL MAGNIFICATION = ocular lens x objective lens. MAGNIFIES UP TO 1000X

Answer 19

compound light microscope

Question 20

_________ __________: uses an electron beam as a source of illumination. uses magnets to focus the beam. CANNOT BE USED ON LIVING SPECIMENS.

Answer 20

electron microscope

Question 21

_________ _________ ______: magnifies up to 1 million and useful for studying interior of the cell

Answer 21

transmission Electron microscope

Question 22

__________ ________ ________: gives extremely detailed 3-D view of objects

Answer 22

Scanning Electron Microscope

Question 23

How to Carry a Microscpe

Answer 23

with one hand on the arm and the other hand underneath the base

Question 24

ONLY use _____ __________ to focus when using high power or oil. NEVER USE ______ _________ when viewing a specimen under high power or oil because it may damage the slides and lens

Answer 24

fine adjustment

course adjustment

Question 25

True or False: you can always return to a lower objective after done with the oil immersion objective

Answer 25

FALSE. NEVER.

AFTER MUST CLEAN OBJECTIVE LENS