Basic Microscopy Flashcards
What microscope is used in the microbiology lab
compound light microscope
Magnification of the ocular lens (eyepiece)
10x
Magnification of the scanning lens (red)
4x
total 40x
Magnification of the low power lens (yellow)
10x
total 100x
Magnification of the high power lens (blue/high dry)
40x
total 400x
Magnification of the Oil Immersion lens (white)
100x
total 1000x
_______ _________: supports objective lenses and rotates them into position
revolving nose piece
______: holds slide
stage
_______ ______ _______: slide holder and knobs that position slide
mechanical stage control
_____ ______ opening in stage that allows light to illuminate
stage aperture
__________: controls the amount of light entering stage aperture
diaphragm
_________ concentrates light source into a cone of light that passes through diaphragm
condenser
______-_________ _____: brings speciment into focus quicly UP AND DOWN
course adjustment knob
______ _________ ______: slowly brings speciment into sharper focus
Fine adjustment knob
_____: supports coarse and fine adjustment knobs, holds head and stage
Arm
______: holds oculars and houses mirrors
head
_____ houses light source, supports and stabilizes entire microscope
base
_______ ___________: use visible light as a source of illumination
light microscope
________ ________: contains more than one magnifying lens. TOTAL MAGNIFICATION = ocular lens x objective lens. MAGNIFIES UP TO 1000X
compound light microscope
_________ __________: uses an electron beam as a source of illumination. uses magnets to focus the beam. CANNOT BE USED ON LIVING SPECIMENS.
electron microscope
_________ _________ ______: magnifies up to 1 million and useful for studying interior of the cell
transmission Electron microscope
__________ ________ ________: gives extremely detailed 3-D view of objects
Scanning Electron Microscope
How to Carry a Microscpe
with one hand on the arm and the other hand underneath the base
ONLY use _____ __________ to focus when using high power or oil. NEVER USE ______ _________ when viewing a specimen under high power or oil because it may damage the slides and lens
fine adjustment
course adjustment
True or False: you can always return to a lower objective after done with the oil immersion objective
FALSE. NEVER.
AFTER MUST CLEAN OBJECTIVE LENS
