match the pathogen with diagnostic technique

Question 1

Majority of fish diseases: gram neg or pos

Answer 1

gram neg.

Question 2

Furunculosis (Aeromonas salmonicida)

GRAM -

Answer 2

O/F +/+
TSA (brown) **
CBB (dark blue) **
ELISA 
PCR

Question 3

Motile Aeromonad Septicemia (MAS) (Aeromonas hydrophila)

GRAM -

Answer 3

TSA **
BHI **
ELISA
O/F +/+ **

Question 4

V. anguillarum, ordali

GRAM -

Answer 4

TSA + 1.5% salt **
MA
TCBS (thiosulphate citreate bile salt sucrose)
VAM 
O/F +/+ **

Question 5

Atypical Ferunculosis

GRAM -

Answer 5

BA

Question 6

V. salmonicida (Hiltra disease)

GRAM -

Answer 6

TSA

BA

Question 7

Myxobacteria = BacGilDis + CldWtrDis

• Flavobacterium
• Tenacibaculum

GRAM -

Answer 7

CA
TA
MHS
FMM
PCR, IFAT, ELISA (BGD only)

Question 8

Enteric Red Mouth (ERM) disease
AKA
Yersinia ruckeri

GRAM -

Answer 8

TSA + 5% blood **
API-20E
ELISA
PCR
Agglutination tests

Question 9

Piscirickettsia salmonid (salmonid rickettsial disease)

GRAM -

Answer 9

CPE(cytopathic effect)**  (kidney cell culture)
liver lesions
histology
FAT
PCR

Question 10

BKD - Renibacterium salmoninarum (Rs)

GRAM +

Answer 10

KDM - long period. **
FAT
ELISA **
Q-PCR (very sensitive) **

Question 11

Mycobacterium chelonae. Mycobacteriosis.

GRAM +

Answer 11

culture, tests, microscopy. NO DETAILS

Question 12

Gaffkemia (red tail disease)
Aerococcus viridans
GRAM +

Shellfish

Answer 12

FAT*****

Question 13

Saprolegnia

Answer 13

observation. cotton like tufts. PROTIST

Question 14

Aphanomyces astaci

Answer 14

OIE LISTED - CFIA IMMEDIATELY NOTIFIABLE
-melanization. observable.
PROTIST

Question 15

Ichthyophonus hoferi

Answer 15

PROTIST

fungal culture essential

Question 16

Fusarium

Answer 16

FUN-GUS
observation?
-‘burn spot disease’ in lobster, shrimp
-‘black gill disease’ local shrimp

Question 17

ASCOMYCETESE - sac fungi

Trichomaris invadens

Answer 17

marine snow crab.
OBSERVATION
-black spots
-form mat

Question 18

IHN (infectious hematopoetic necrosis)

RNA, envelloped

Answer 18

FHM** - 15 days
EPC** - 15 days
CHSE** - 15 days
RT-PCR** - 2 days

This disease is lethal in 4-7 days

Question 19

ISA (infectious salmon anemia)

RNA, enveloped

Answer 19

pathology
CPE on SHK**
IFAT
RT-PCR **

Question 20

VHS - Viral Hemorrhagic Septicimia
RNA
enveloped

Answer 20

pathology
CPE on RTG, FHM, CHSE
ELISA
IFAT 
RT-PCR

Question 21

VNN - VIral Nervous Necrosis
RNA
NON-ENVELOPED

Answer 21

histopathology
CPE on SSN-1**
RT-PCR
ELISA

Question 22

IPN Infectious Pancreatic Necrosis
RNA
NON-ENVELOPED

Answer 22

pathology
CPE on RTG or CHSE
IFAT
RT-PCR

Question 23

WSIV - white sturgeon iridovirus
DNA
NON-ENVELOPED

Answer 23

CPE in WSS-2 (white sturgeon spleen) or WSSK-1 (white sturgeon skin)**
histology

Question 24

LCD - Lymphocystis disease
DNA
NON-ENVELOPED

Answer 24

numerous cysts and tumor like lesions on body

Question 25

OVVD - oyster velar viral disease

DNA, non-enveloped

Answer 25

lesions (blisters) near VELUM