M8 Formative

Question 1

What is not true about the genetically modified ‘Btcrops’?

The technique has been applied to several species of crops
The source organism for the gene is a soil bacterium
The transferred gene exists in several allelic forms
Bt brinjal has been commercially exploited

Answer 1

Bt brinjal has been commercially exploited

Question 2

Golden rice is a genetically modified crop plant where the incorporated genes are meant for biosynthesis of ——-.

Answer 2

beta-carotene

Question 3

Which organism among the following was not used in the genetic engineering programmeleading to the development of Golden Rice?

Escherichia coli

Erwinia uredovora

Agrobacterium tumefaciens

Narcissus pseudonarcissus

Answer 3

Escherichia coli

Question 4

——-is the product of RNAitechnique in genetic engineering.

Cry proteins of Bt cotton

Cry proteins of Bt brinjal

Beta carotene enriched Golden rice

Nematode resistance in tobacco

Answer 4

Beta carotene enriched Golden rice

Question 5

Interference RNA was discovered by

Answer 5

Fire & Mello

Question 6

Identify the organism in which RNAiwas first discovered

Meloidegyne incognitia

Escherichia coli

Bacillus thuringiensis

Coenorhabditis elegans

Answer 6

Coenorhabditis elegans

Question 7

The function of Interference RNA to prevent a gene from functioning is also known as

Reverse transcription

Epigenetics

PTGS (Post Transcriptional Gene Silencing)

RNA splicing

Answer 7

PTGS (Post Transcriptional Gene Silencing)

Question 8

This is not a product of recombinant DNA technology:

Golden rice
Tracy
Bt cotton
Dolly

Answer 8

Dolly

Question 9

This is not a GMO.

Bt cotton
Golden rice
Tracy
Pomato

Answer 9

pomato

Question 10

In the famous rDNAexperiment to produce humulin, the substance was produced attached with another protein called ——-

beta-galactosidase

beta-galactoside

ampicillin

tetracycline

Answer 10

beta-galactosidase

Question 11

The active form of human insulin consists of these segments of polypeptide chains.

Answer 11

A & B Chains

Question 12

In the famous example of human gene therapy for SCID disease, the procedure adopted was:

ex vivo germ cell therapy

ex vivo somatic cell therapy

in vivo germ cell therapy

in vivosomatic cell therapy

Answer 12

ex vivo somatic cell therapy

Question 13

What is the advantage in clinical use of humulin(human insulin produced through rDNAtechnique) over use of conventional ox or pig insulin?

It does not cause immunological problems

It is cheaper for the patient

It is produced by E.coliin our own intestine

There is no advantage

Answer 13

It does not cause immunological problems

Question 14

Gene therapy for SCID disease in man involves the transfer of the gene for this enzyme

Beta-galactosidase

Thrombokinase

Adenosine deaminase

Phenylalanine hydroxylase

Answer 14

Beta-galactosidase

Question 15

Spot the mismatch between the organisms with the actual modifications done:

Rosie –Lactalbumin enriched milk

Tracy –Human AAT protein enriched milk

GM Mice -Cry protein enrichment

Golden rice –beta-carotene enrichment

Answer 15

GM Mice -Cry protein enrichment

Question 16

One of the advantages of developing transgenic mice is it is very useful in:

Answer 16

Gene targeting

Question 17

When these parts of a plant are used as explants in a tissue culture experiments the newly generated plants could be virus-free

Answer 17

Meristems

Question 18

Haploid plants could be produced from tissue culture of the following parts of a healthy plant

Answer 18

Pollen grains

Question 19

Normally this is not expected in the new plants produced through tissue culture programmes:

Answer 19

Variations in genotype

Question 20

Tissue culture experiments have to be conducted in extremely aseptic conditions. Therefore all things used in the tissue culture programmemust be properly sterilized before use with the exception of ————–

The explant

The culture vessel

The various instruments used

The nutrient medium

Answer 20

The explant

Question 21

What is not true about the genetically modified ‘Bt crops’?

Answer 21

Bt brinjal has been commercially exploited

Question 22

. The first successful preparation of recombinant DNA using restriction enzymes
was achieved by

Answer 22

Cohen & Boyer

Question 23

Which of the following is the name of a restriction endonuclease?

Answer 23

Eco RI

Question 24

Which of the following statements is not true for RENs?

They are naturally available in bacterial cytoplasm.

They recognize palindromic sequences of nucleotides on DNA.

They can cut DNA at any place.

They are popularly known as molecular scissors.

Answer 24

They can cut DNA at any place.

Question 25

An REN cuts a DNA molecule only at this region:

Answer 25

restriction site

Question 26

The following sequences of nucleotides at different regions on one strand of a
DNA molecule. Which of them is likely to be recognized and cut by a restriction
enzyme?

ATATTA
CCTAGG
GGGTTT
CATCAT

Answer 26

CCTAGG