M8 Formative Flashcards
What is not true about the genetically modified ‘Btcrops’?
The technique has been applied to several species of crops
The source organism for the gene is a soil bacterium
The transferred gene exists in several allelic forms
Bt brinjal has been commercially exploited
Bt brinjal has been commercially exploited
Golden rice is a genetically modified crop plant where the incorporated genes are meant for biosynthesis of ——-.
beta-carotene
Which organism among the following was not used in the genetic engineering programmeleading to the development of Golden Rice?
Escherichia coli
Erwinia uredovora
Agrobacterium tumefaciens
Narcissus pseudonarcissus
Escherichia coli
——-is the product of RNAitechnique in genetic engineering.
Cry proteins of Bt cotton
Cry proteins of Bt brinjal
Beta carotene enriched Golden rice
Nematode resistance in tobacco
Beta carotene enriched Golden rice
Interference RNA was discovered by
Fire & Mello
Identify the organism in which RNAiwas first discovered
Meloidegyne incognitia
Escherichia coli
Bacillus thuringiensis
Coenorhabditis elegans
Coenorhabditis elegans
The function of Interference RNA to prevent a gene from functioning is also known as
Reverse transcription
Epigenetics
PTGS (Post Transcriptional Gene Silencing)
RNA splicing
PTGS (Post Transcriptional Gene Silencing)
This is not a product of recombinant DNA technology:
Golden rice
Tracy
Bt cotton
Dolly
Dolly
This is not a GMO.
Bt cotton
Golden rice
Tracy
Pomato
pomato
In the famous rDNAexperiment to produce humulin, the substance was produced attached with another protein called ——-
beta-galactosidase
beta-galactoside
ampicillin
tetracycline
beta-galactosidase
The active form of human insulin consists of these segments of polypeptide chains.
A & B Chains
In the famous example of human gene therapy for SCID disease, the procedure adopted was:
ex vivo germ cell therapy
ex vivo somatic cell therapy
in vivo germ cell therapy
in vivosomatic cell therapy
ex vivo somatic cell therapy
What is the advantage in clinical use of humulin(human insulin produced through rDNAtechnique) over use of conventional ox or pig insulin?
It does not cause immunological problems
It is cheaper for the patient
It is produced by E.coliin our own intestine
There is no advantage
It does not cause immunological problems
Gene therapy for SCID disease in man involves the transfer of the gene for this enzyme
Beta-galactosidase
Thrombokinase
Adenosine deaminase
Phenylalanine hydroxylase
Beta-galactosidase
Spot the mismatch between the organisms with the actual modifications done:
Rosie –Lactalbumin enriched milk
Tracy –Human AAT protein enriched milk
GM Mice -Cry protein enrichment
Golden rice –beta-carotene enrichment
GM Mice -Cry protein enrichment
One of the advantages of developing transgenic mice is it is very useful in:
Gene targeting
When these parts of a plant are used as explants in a tissue culture experiments the newly generated plants could be virus-free
Meristems
Haploid plants could be produced from tissue culture of the following parts of a healthy plant
Pollen grains
Normally this is not expected in the new plants produced through tissue culture programmes:
Variations in genotype
Tissue culture experiments have to be conducted in extremely aseptic conditions. Therefore all things used in the tissue culture programmemust be properly sterilized before use with the exception of ————–
The explant
The culture vessel
The various instruments used
The nutrient medium
The explant
What is not true about the genetically modified ‘Bt crops’?
Bt brinjal has been commercially exploited
. The first successful preparation of recombinant DNA using restriction enzymes
was achieved by
Cohen & Boyer
Which of the following is the name of a restriction endonuclease?
Eco RI
Which of the following statements is not true for RENs?
They are naturally available in bacterial cytoplasm.
They recognize palindromic sequences of nucleotides on DNA.
They can cut DNA at any place.
They are popularly known as molecular scissors.
They can cut DNA at any place.
An REN cuts a DNA molecule only at this region:
restriction site
The following sequences of nucleotides at different regions on one strand of a
DNA molecule. Which of them is likely to be recognized and cut by a restriction
enzyme?
ATATTA
CCTAGG
GGGTTT
CATCAT
CCTAGG
