Lesson 26 - IAM calculations

Question 1

Two objects are 0.3µm apart, will a light microscope be able to distinguish between these two objects?

Answer 1

yes

Question 2

Two objects are 0.1µm apart, will a light microscope be able to distinguish between these two objects?

Answer 2

no

Question 3

What is the difference between magnification and resolution?

Answer 3

Magnification = the degree to which the size of an image is larger than the object itself.
Resolution ( or resolving power) = The minimum distance apart that 2 objects can be in order for them to appear as separate items. The higher the resolution, the greater the detail you can see.

Question 4

Convert 0.25mm to micrometres (µm)

Answer 4

250 µm

Question 5

what are the steps to calibrate a microscope?

Answer 5

1. It’s not practical to have a ruler on the stage all the time, and 1mm is NOT precise enough. So we use an stage micrometre.
2. When you put the stage micrometre onto the microscope stage, the eyepiece graticule and stage micrometer are at different magnifications.
3. At each magnification (change the objective lenses) work out how many micrometers are equal to 1epu (now you have your calibration)

Question 6

What is the IAM formula?

Answer 6

magnification = image size/actual size