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Clinical Bacteriology (Prelim) > (Lecture) Culture Media > Flashcards

(Lecture) Culture Media Flashcards

1
Q

It is the media containing the required nutrients for bacterial growth

A

Culture Media

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2
Q

Serves as the soil in which microbes can be planted and grown.

A

Culture Media

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3
Q

Uses of Culture Media:

A

 Isolation and identification of microorganisms
 Performing anti-microbial sensitivity tests

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4
Q

List down the Common Ingredients of a Culture Media:

A

 Peptone
 Meat extract
 Yeast extract
 Mineral salts
 Carbohydrates
 Agar
 Water

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5
Q

Extracts containing hydrolyzed plant and animal proteins that serve as source of amino acids, peptides, and proteins for the bacteria.

A

Peptone

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6
Q

A Common Ingredient that provides vitamins and mineral salts necessary for growth.

A

Meat Extract

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7
Q

A Common Ingredient that serves as a bacterial growth stimulants

A

Yeast Extracts

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8
Q

They provide inorganic compounds such as phosphates and sulfur necessary for bacterial metabolism.

A

Mineral Salts

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9
Q

It is a simple and complex sugars that are a source of carbon and energy. They also assist in the differentiation of bacteria.

A

Carbohydrates

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10
Q

This differentiates Vibrio species

A

Sucrose in TCBS (Thiosulfate-Citrate-Bile Salts-Sucrose) Agar

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11
Q

This differentiates Enterobacteriaceae

A

Lactose in MacConkey Agar

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12
Q

A polymer that acts as a solidifying agent in culture media and is not metabolized by the growing organisms.

A

Agar

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13
Q

Properties of Agar:

A

o It has high gelling strength
o High melting temperature (90-95 degree Celsius)
o Low gelling temperature
o It forms firm gel at 1.5% W/V concentration
o It forms semisolid gel at 0.4-0.5% W/V concentration

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14
Q

Uses of Agar:

A

o Solidify culture media
o May provide calcium and organic ions to inoculated bacteria

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15
Q

What Type of Water must be used in preparation of Culture Media?

A

Deionized or Distilled Water

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16
Q

Types of Culture Media based on Consistency:

A

 Liquid (broth)
 Solid (agar)
 Semisolid

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17
Q

Types of Culture Media based on Nutritional Factors:

A

 Simple (basal)
 Complex
 Synthetic or defined

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18
Q

Types of Special Media:

A

 Enriched
 Enrichment
 Selective
 Indicator or differential
 Transport
 Sugar media

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19
Q

A Culture Media wherein the nutrients are dissolved in water, and bacterial growth is indicated by a change in broth’s appearance from clear to turbid

A

Liquid (Broth) Media

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20
Q

Uses of Liquid (Broth) Media:

A

o As an enrichment media
o As biochemical testing media
o As blood culture media

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21
Q

A Culture Media that is made by adding a solidifying agent to the nutrients and water.

A

Solid (Agar) Media

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22
Q

The most common solidifying agent

A

Agarose

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23
Q

Solid Media contain how many percent of agar?

A

2% to 3%

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24
Q

Small colonies is identified as _____

A

Pinpoint Colonies

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25
Q

Large Colonies is identified by _____

A

Pinhead Colonies

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26
Q

A Type of Culture media wherein agar is added to the media in concentrations that are too low to solidify them.

A

Semisolid Culture Media

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27
Q

How many percent of Agar does a Semi-solid Media contains?

A

0.5% to 1%

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28
Q

Indicated by the spread or extension of the microorganisms from the stud line and produce turbidity throughout the media.

A

Motility

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29
Q

Uses of Semi-solid Media:

A

o As an enrichment media
o As motility media

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30
Q

A media that contains only basic nutrients required for the growth of ordinary organisms, and used as a general purpose media

A

Basic/Simple/All-purpose Media

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31
Q

Uses of Basic/Simple/All-purpose Media:

A

o To prepare enriched media
o To maintain stock cultures of control bacterial strains
o To subcuture pathogenic bacteria from
selective/differential medium prior to performing biochemical or serological tests

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32
Q

Media that contain some ingredients of unknown chemical composition

A

Complex Media

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33
Q

One common ingredient of Complex Media

A

Peptone

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34
Q

Commonly used complex medium

A

Nutrient Broth

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35
Q

A simple basal liquid medium, supports growth of many organisms.

A

Nutrient Broth

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36
Q

Types of Nutrient Broth:

A

 Meat infusion broth
 Meat extract broth
 Digest Broth

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37
Q

Solid counterpart of nutrient broth and it is used to grow non-fastidious bacteria or organisms that have no additional requirement for growth and isolation.

A

Nutrient Agar

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38
Q

Nutrient Agar is prepared by adding agar at a concentration of _____ to the Nutrient Broth

A

2%

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39
Q

Nutrient Agar is prepared by adding agar at a concentration of 2% to the _____

A

Nutrient Broth

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40
Q

It is simplest and most common medium used routinely in microbiology laboratories, to grow nonfastidious bacteria

A

Nutrient Agar

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41
Q

Nutrient Agar is commonly referred to as _____

A

Agar Medium

42
Q

A Media that is prepared exclusively from pure chemical substances and their exact composition is known.

A

Synthetic or Chemically Defined Media

43
Q

A Media that is prepared to meet the nutritional requirements of more bacteria by the addition of substances such as blood, serum or egg to a basal medium.

A

Enriched Media

44
Q

Identify the Type of Enriched Media based on the information given below:

 Many bacteria are fastidious, requiring
a medium that is even richer than
nutrient agar commonly used in
clinical laboratories.
 Used for isolation of streptococci,
pneumococci, Haemophilus

A

Blood Agar

45
Q

Identify the Type of Enriched Media based on the information given below:

  • Used to culture even more fastidious bacteria.
  • It is used for isolation of Neisseria (Meningococci and Gonococci) and Haemophilus.
A

Chocolate Agar

46
Q

Identify the Type of Enriched Media based on the information given below:

 Serum is added for enriching the
medium.
 Used for the isolation of
Corynebacterium diphtheriae.

A

Loeffler’s Serum

47
Q

Substance added to a liquid medium which inhibits the growth of unwanted bacteria and favors the growth of wanted bacteria. This is usually liquid and provides nutrients and environmental conditions that favor the growth of a particular microbe but not others.

A

Enrichment Media

48
Q

An Enrichment Media that Inhibits coliforms while allowing typhoidparatyphoid bacilli to grow freely in fecal sample.

A

Tetrathionate Broth

49
Q

An Enrichment Media that is used for dysentery bacilli

A

Selenite F (F for feces) Broth

50
Q

An Enrichment Media that is used for Vibrio cholerae from feces.

A

Alkaline Peptone Water

51
Q

An Enrichment Media used for the isolation of Salmonella and Proteus species from stool samples.

A

Tetrathionate Broth

52
Q

pH level of _____ Alkaline Peptone Water that is used to promote growth Vibrio species

A

8.5

53
Q

Substance is added to a solid medium which inhibits the growth of unwanted bacteria but favors the growth of wanted bacteria. It is used to isolate particular bacteria from specimens where mixed bacterial flora is expected.

A

Selective Media

54
Q

A Selective Media that acts as a selective agent for dysentery bacilli (isolation of Shigellae).

A

Desoxycholate Citrate Agar (DCA)

55
Q

A Selective Media used for Mycobacterium Tuberculosis

A

Lowenstein-Jensen Medium

56
Q

A Selective Media that acts as a selective agent and allow the the growth of only
Vibrio cholerae and inhibits the growth of
intestinal organisms.

A

Bile Salt Agar (BSA)

57
Q

A Media that contains an which changes color when a bacterium grows in them.

A

Indicator Media

58
Q

An Indicator Media that incorporates sulfite, S. typhi reduces sulfite to sulfide in the presence of glucose and the colonies of S. typhi have a black metallic sheen.

A

Wilson and Blair Medium

59
Q

An Indicator Media that indicates lactose fermenting property, lactose fermenter (LF) produces pink colonies and non-lactose fermenter (NLF) produces colorless colonies due to a neutral red indicator

A

MacConkey Agar

60
Q

A Type of Media that has substances incorporated in it, enabling it to bring out differing characteristics of bacteria and thus helping to distinguish between them

A

Differential Media

61
Q

_____ is both differential and selective, contains peptone, meat extract, NACl, bile salt, lactose and neutral red indicator.

A

MacConkey Agar

62
Q

A Media that is used to test the fermentation

A

Sugar Media

63
Q

These are routinely employed for fermentation test

A

Glucose
Lactose
Sucrose
Mannitol

64
Q

Color of the medium in Sugar Media

A

Light Yellow

65
Q

In Sugar Media, acid production is indicated by the development of _____

A

Pink Color

66
Q

A small inverted test tube placed inside a larger tube containing the media.

A

Durham’s Tube

67
Q

Are culture media used to determine sugar fermentation of fastidious organisms such as Neisseria and streptococcus species.

A

Hiss Serum Sugar

68
Q

Media containing ingredients to prevent the overgrowth of commensals and ensure the survival of pathogenic bacteria when specimens cannot be cultured soon after
collection.

A

Transport Media

69
Q

List down the Types of Transport Media:

A
  1. Amies Transport Media
  2. Stuart Media
  3. Kelly-Blair Media
70
Q

A special media that enhances the growth of anaerobic microorganisms.

A

Anaerobic Media

71
Q

A Type of Medium under Anaerobic Media that contains reducing agents, such as sodium thioglycollate, glucose, vitamin C (ascorbic acid), cysteine and agar (concentration of 0.05%), with methylene blue

A

Thioglycollate Broth

72
Q

_____ or _____ act as an oxidation reduction potential indicator, which should show that the medium is anerobic except in the surface layer in addition to a reducing agent and semisolid agar.

A

Methylene Blue or Resazurin

73
Q

A Type of Medium under Anaerobic Media that is used for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species.

A

Cooked Meat Broth or RCM Broth (Robertson’s Cooked Meat Broth)

74
Q

A Type of Media under Anaerobic Media that supports the growth of both spore-forming and nonspore forming obligate anaerobes and also differentiate between putrefactive and saccharolytic species.

A

Cooked Meat Broth or RCM Broth (Robertson’s Cooked Meat Broth)

75
Q

Identify the Medium based on the characteristic given:

 Basis for carbohydrate fermentation media
 For testing the formation of indole

A

Peptone Water

76
Q

Identify the Medium based on the characteristic given:

 For routine culture
 As a base to prepare many other culture
media
 To study growth curve

A

Nurient Broth

77
Q

Identify the Medium based on the characteristic given:

 Blood culture
 Promotes luxuriant growth of many
organisms
 Glucose acts as a reducing agent

A

Glucose Broth

78
Q

Identify the Medium based on the characteristic given:

Enriches salmonellae and sometimes shigellae

A

Tetrathionate Broth

79
Q

Identify the Medium based on the characteristic given:

 Inhibits coliform bacilli while permitting
salmonellae and many shigellae to grow

A

Selenite F Broth

80
Q

Identify the Medium based on the characteristic given:

 Excellent medium for enriching the number of V. cholera and other vibrio
species in a fecal specimen.

A

Alkaline Peptone Water

81
Q

Identify the Medium based on the characteristic given:

Supports growth of anaerobes, aerobes, microaerophillic and fastidious microorganisms

A

Thioglycollate Broth

82
Q

Identify the Medium based on the characteristic given:

 Culture of anaerobic bacteria
 Preservation of stock culture of aerobic bacteria

A

Robertson’s Cooked Meat Broth (RCM)

83
Q

Identify the Medium based on the characteristic given:

Complex medium used for routine laboratory work

A

Nutrient Agar

84
Q

Identify the Medium based on the characteristic given:

In addition to being enriched medium, it
is an indicator medium showing the hemolytic properties of bacteria

A

Blood Agar

85
Q

Identify the Medium based on the characteristic given:

This medium is used to culture fastidious
bacteria, such as Haemophilus influenza, the neisseriae and Pneumococcus

A

Chocolate Agar

86
Q

Identify the Medium based on the characteristic given:

Culture of Corynebacterium diphtheria

A

Loeffler’s Serum Slope (LSS)

87
Q

Identify the Medium based on the characteristic given:

Isolation and differentiation of lactose fermenting (LF) and nonlactose fermenting (NLF) enteric bacilli. It is selective and differential.

A

MacConkey Agar

88
Q

Identify the Medium based on the characteristic given:

Suitable for the isolation of Salmonella and Shigella

A

Desoxycholate Citrate Agar (DCA)

89
Q

Identify the Medium based on the characteristic given:

Culture of Vibrio cholerae

A

Bile Salt Agar (BSA)

90
Q

List down the steps for the Preparation of Culture Media:

A
  1. Weighing and dissolving of culture media ingredients
  2. Sterilization and sterility testing
  3. Dispensing of Culture Media
91
Q

Methods used to Sterilize Culture Media:

A
  1. Autoclaving
  2. Steaming to 100 degree Celsius
  3. Filtration
92
Q

A Method of Sterilization that is used to sterilize most agar and fluid culture media.

A

Autoclaving

93
Q

Preferred time and temperature of Autoclaving

A

121 degree Celsius, 15psi for not less than 15 minutes

94
Q

A Sterilization Method that is used to sterilize media containing ingredients that would be inactivated at temperature over 100°C and re-melt previously bottled sterile agar media.

A

Steaming at 100 degree Celsius

95
Q

A Sterilization Method that is used to sterilize additives that are heat-sensitive and cannot be autoclaved.

A

Filtration

96
Q

The simplest way to test for contamination is to incubate the prepared sample media at _____

A

35 to 37 degree Celsius for 24 hours

97
Q

After autoclaving, heat sensitive ingredients are added when the medias cooled at _____

A

50 degree Celsius

98
Q

pH can be tested with the use of _____ or _____

A

pH Paper
pH Meter

99
Q

Plates of culture media, and additives like serum, blood and antimicrobials in solid form require storage at _____

A

2 to 8 degree Celsius

100
Q

Antimicrobials in solution form should be stored at _____

A

-2 degree Celsius

101
Q

The temperature selected for routine culturing is _____

A

35 to 37 degree Celsius

102
Q

Some pathogens require CO2-enriched atmosphere to grow - to enclose a lighted candle in an airtight jar which provides _____ CO2 by the time the candle is
extinguished.

A

3% to 5%

Clinical Bacteriology (Prelim) (17 decks)

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