(Lecture) Culture Media Flashcards
1
Q
It is the media containing the required nutrients for bacterial growth
A
Culture Media
2
Q
Serves as the soil in which microbes can be planted and grown.
A
Culture Media
3
Q
Uses of Culture Media:
A
Isolation and identification of microorganisms
Performing anti-microbial sensitivity tests
4
Q
List down the Common Ingredients of a Culture Media:
A
Peptone
Meat extract
Yeast extract
Mineral salts
Carbohydrates
Agar
Water
5
Q
Extracts containing hydrolyzed plant and animal proteins that serve as source of amino acids, peptides, and proteins for the bacteria.
A
Peptone
6
Q
A Common Ingredient that provides vitamins and mineral salts necessary for growth.
A
Meat Extract
7
Q
A Common Ingredient that serves as a bacterial growth stimulants
A
Yeast Extracts
8
Q
They provide inorganic compounds such as phosphates and sulfur necessary for bacterial metabolism.
A
Mineral Salts
9
Q
It is a simple and complex sugars that are a source of carbon and energy. They also assist in the differentiation of bacteria.
A
Carbohydrates
10
Q
This differentiates Vibrio species
A
Sucrose in TCBS (Thiosulfate-Citrate-Bile Salts-Sucrose) Agar
11
Q
This differentiates Enterobacteriaceae
A
Lactose in MacConkey Agar
12
Q
A polymer that acts as a solidifying agent in culture media and is not metabolized by the growing organisms.
A
Agar
13
Q
Properties of Agar:
A
o It has high gelling strength
o High melting temperature (90-95 degree Celsius)
o Low gelling temperature
o It forms firm gel at 1.5% W/V concentration
o It forms semisolid gel at 0.4-0.5% W/V concentration
14
Q
Uses of Agar:
A
o Solidify culture media
o May provide calcium and organic ions to inoculated bacteria
15
Q
What Type of Water must be used in preparation of Culture Media?
A
Deionized or Distilled Water
16
Q
Types of Culture Media based on Consistency:
A
Liquid (broth)
Solid (agar)
Semisolid
17
Q
Types of Culture Media based on Nutritional Factors:
A
Simple (basal)
Complex
Synthetic or defined
18
Q
Types of Special Media:
A
Enriched
Enrichment
Selective
Indicator or differential
Transport
Sugar media
19
Q
A Culture Media wherein the nutrients are dissolved in water, and bacterial growth is indicated by a change in broth’s appearance from clear to turbid
A
Liquid (Broth) Media
20
Q
Uses of Liquid (Broth) Media:
A
o As an enrichment media
o As biochemical testing media
o As blood culture media
21
Q
A Culture Media that is made by adding a solidifying agent to the nutrients and water.
A
Solid (Agar) Media
22
Q
The most common solidifying agent
A
Agarose
23
Q
Solid Media contain how many percent of agar?
A
2% to 3%
24
Q
Small colonies is identified as _____
A
Pinpoint Colonies
25
Large Colonies is identified by _____
Pinhead Colonies
26
A Type of Culture media wherein agar is added to the media in concentrations that are too low to solidify them.
Semisolid Culture Media
27
How many percent of Agar does a Semi-solid Media contains?
0.5% to 1%
28
Indicated by the spread or extension of the microorganisms from the stud line and produce turbidity throughout the media.
Motility
29
Uses of Semi-solid Media:
o As an enrichment media
o As motility media
30
A media that contains only basic nutrients required for the growth of ordinary organisms, and used as a general purpose media
Basic/Simple/All-purpose Media
31
Uses of Basic/Simple/All-purpose Media:
o To prepare enriched media
o To maintain stock cultures of control bacterial strains
o To subcuture pathogenic bacteria from
selective/differential medium prior to performing biochemical or serological tests
32
Media that contain some ingredients of unknown chemical composition
Complex Media
33
One common ingredient of Complex Media
Peptone
34
Commonly used complex medium
Nutrient Broth
35
A simple basal liquid medium, supports growth of many organisms.
Nutrient Broth
36
Types of Nutrient Broth:
Meat infusion broth
Meat extract broth
Digest Broth
37
Solid counterpart of nutrient broth and it is used to grow non-fastidious bacteria or organisms that have no additional requirement for growth and isolation.
Nutrient Agar
38
Nutrient Agar is prepared by adding agar at a concentration of _____ to the Nutrient Broth
2%
39
Nutrient Agar is prepared by adding agar at a concentration of 2% to the _____
Nutrient Broth
40
It is simplest and most common medium used routinely in microbiology laboratories, to grow nonfastidious bacteria
Nutrient Agar
41
Nutrient Agar is commonly referred to as _____
Agar Medium
42
A Media that is prepared exclusively from pure chemical substances and their exact composition is known.
Synthetic or Chemically Defined Media
43
A Media that is prepared to meet the nutritional requirements of more bacteria by the addition of substances such as blood, serum or egg to a basal medium.
Enriched Media
44
Identify the Type of Enriched Media based on the information given below:
Many bacteria are fastidious, requiring
a medium that is even richer than
nutrient agar commonly used in
clinical laboratories.
Used for isolation of streptococci,
pneumococci, Haemophilus
Blood Agar
45
Identify the Type of Enriched Media based on the information given below:
- Used to culture even more fastidious bacteria.
- It is used for isolation of Neisseria (Meningococci and Gonococci) and Haemophilus.
Chocolate Agar
46
Identify the Type of Enriched Media based on the information given below:
Serum is added for enriching the
medium.
Used for the isolation of
Corynebacterium diphtheriae.
Loeffler's Serum
47
Substance added to a liquid medium which inhibits the growth of unwanted bacteria and favors the growth of wanted bacteria. This is usually liquid and provides nutrients and environmental conditions that favor the growth of a particular microbe but not others.
Enrichment Media
48
An Enrichment Media that Inhibits coliforms while allowing typhoidparatyphoid bacilli to grow freely in fecal sample.
Tetrathionate Broth
49
An Enrichment Media that is used for dysentery bacilli
Selenite F (F for feces) Broth
50
An Enrichment Media that is used for Vibrio cholerae from feces.
Alkaline Peptone Water
51
An Enrichment Media used for the isolation of Salmonella and Proteus species from stool samples.
Tetrathionate Broth
52
pH level of _____ Alkaline Peptone Water that is used to promote growth Vibrio species
8.5
53
Substance is added to a solid medium which inhibits the growth of unwanted bacteria but favors the growth of wanted bacteria. It is used to isolate particular bacteria from specimens where mixed bacterial flora is expected.
Selective Media
54
A Selective Media that acts as a selective agent for dysentery bacilli (isolation of Shigellae).
Desoxycholate Citrate Agar (DCA)
55
A Selective Media used for Mycobacterium Tuberculosis
Lowenstein-Jensen Medium
56
A Selective Media that acts as a selective agent and allow the the growth of only
Vibrio cholerae and inhibits the growth of
intestinal organisms.
Bile Salt Agar (BSA)
57
A Media that contains an which changes color when a bacterium grows in them.
Indicator Media
58
An Indicator Media that incorporates sulfite, S. typhi reduces sulfite to sulfide in the presence of glucose and the colonies of S. typhi have a black metallic sheen.
Wilson and Blair Medium
59
An Indicator Media that indicates lactose fermenting property, lactose fermenter (LF) produces pink colonies and non-lactose fermenter (NLF) produces colorless colonies due to a neutral red indicator
MacConkey Agar
60
A Type of Media that has substances incorporated in it, enabling it to bring out differing characteristics of bacteria and thus helping to distinguish between them
Differential Media
61
_____ is both differential and selective, contains peptone, meat extract, NACl, bile salt, lactose and neutral red indicator.
MacConkey Agar
62
A Media that is used to test the fermentation
Sugar Media
63
These are routinely employed for fermentation test
Glucose
Lactose
Sucrose
Mannitol
64
Color of the medium in Sugar Media
Light Yellow
65
In Sugar Media, acid production is indicated by the development of _____
Pink Color
66
A small inverted test tube placed inside a larger tube containing the media.
Durham's Tube
67
Are culture media used to determine sugar fermentation of fastidious organisms such as Neisseria and streptococcus species.
Hiss Serum Sugar
68
Media containing ingredients to prevent the overgrowth of commensals and ensure the survival of pathogenic bacteria when specimens cannot be cultured soon after
collection.
Transport Media
69
List down the Types of Transport Media:
1. Amies Transport Media
2. Stuart Media
3. Kelly-Blair Media
70
A special media that enhances the growth of anaerobic microorganisms.
Anaerobic Media
71
A Type of Medium under Anaerobic Media that contains reducing agents, such as sodium thioglycollate, glucose, vitamin C (ascorbic acid), cysteine and agar (concentration of 0.05%), with methylene blue
Thioglycollate Broth
72
_____ or _____ act as an oxidation reduction potential indicator, which should show that the medium is anerobic except in the surface layer in addition to a reducing agent and semisolid agar.
Methylene Blue or Resazurin
73
A Type of Medium under Anaerobic Media that is used for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species.
Cooked Meat Broth or RCM Broth (Robertson's Cooked Meat Broth)
74
A Type of Media under Anaerobic Media that supports the growth of both spore-forming and nonspore forming obligate anaerobes and also differentiate between putrefactive and saccharolytic species.
Cooked Meat Broth or RCM Broth (Robertson's Cooked Meat Broth)
75
Identify the Medium based on the characteristic given:
Basis for carbohydrate fermentation media
For testing the formation of indole
Peptone Water
76
Identify the Medium based on the characteristic given:
For routine culture
As a base to prepare many other culture
media
To study growth curve
Nurient Broth
77
Identify the Medium based on the characteristic given:
Blood culture
Promotes luxuriant growth of many
organisms
Glucose acts as a reducing agent
Glucose Broth
78
Identify the Medium based on the characteristic given:
Enriches salmonellae and sometimes shigellae
Tetrathionate Broth
79
Identify the Medium based on the characteristic given:
Inhibits coliform bacilli while permitting
salmonellae and many shigellae to grow
Selenite F Broth
80
Identify the Medium based on the characteristic given:
Excellent medium for enriching the number of V. cholera and other vibrio
species in a fecal specimen.
Alkaline Peptone Water
81
Identify the Medium based on the characteristic given:
Supports growth of anaerobes, aerobes, microaerophillic and fastidious microorganisms
Thioglycollate Broth
82
Identify the Medium based on the characteristic given:
Culture of anaerobic bacteria
Preservation of stock culture of aerobic bacteria
Robertson's Cooked Meat Broth (RCM)
83
Identify the Medium based on the characteristic given:
Complex medium used for routine laboratory work
Nutrient Agar
84
Identify the Medium based on the characteristic given:
In addition to being enriched medium, it
is an indicator medium showing the hemolytic properties of bacteria
Blood Agar
85
Identify the Medium based on the characteristic given:
This medium is used to culture fastidious
bacteria, such as Haemophilus influenza, the neisseriae and Pneumococcus
Chocolate Agar
86
Identify the Medium based on the characteristic given:
Culture of Corynebacterium diphtheria
Loeffler's Serum Slope (LSS)
87
Identify the Medium based on the characteristic given:
Isolation and differentiation of lactose fermenting (LF) and nonlactose fermenting (NLF) enteric bacilli. It is selective and differential.
MacConkey Agar
88
Identify the Medium based on the characteristic given:
Suitable for the isolation of Salmonella and Shigella
Desoxycholate Citrate Agar (DCA)
89
Identify the Medium based on the characteristic given:
Culture of Vibrio cholerae
Bile Salt Agar (BSA)
90
List down the steps for the Preparation of Culture Media:
1. Weighing and dissolving of culture media ingredients
2. Sterilization and sterility testing
3. Dispensing of Culture Media
91
Methods used to Sterilize Culture Media:
1. Autoclaving
2. Steaming to 100 degree Celsius
3. Filtration
92
A Method of Sterilization that is used to sterilize most agar and fluid culture media.
Autoclaving
93
Preferred time and temperature of Autoclaving
121 degree Celsius, 15psi for not less than 15 minutes
94
A Sterilization Method that is used to sterilize media containing ingredients that would be inactivated at temperature over 100°C and re-melt previously bottled sterile agar media.
Steaming at 100 degree Celsius
95
A Sterilization Method that is used to sterilize additives that are heat-sensitive and cannot be autoclaved.
Filtration
96
The simplest way to test for contamination is to incubate the prepared sample media at _____
35 to 37 degree Celsius for 24 hours
97
After autoclaving, heat sensitive ingredients are added when the medias cooled at _____
50 degree Celsius
98
pH can be tested with the use of _____ or _____
pH Paper
pH Meter
99
Plates of culture media, and additives like serum, blood and antimicrobials in solid form require storage at _____
2 to 8 degree Celsius
100
Antimicrobials in solution form should be stored at _____
-2 degree Celsius
101
The temperature selected for routine culturing is _____
35 to 37 degree Celsius
102
Some pathogens require CO2-enriched atmosphere to grow - to enclose a lighted candle in an airtight jar which provides _____ CO2 by the time the candle is
extinguished.
3% to 5%
