(Laboratory) Smear Preparation and Simple Staining

Question 1

List down the step-by-step method for Plate Smear Preparation

Answer 1

1. Label your slides.
2. Place a loopful of NSS onto the slide.
3. Emulsify an isolate colony. Air dry.
4. Heat fix.

Question 2

List down the step-by-step method for Broth Smear Preparation

Answer 2

1. Label your slides.
2. Place a loopful of broth and air dry.
3. Heat fix.

Question 3

List down the step-by-step procedure for Urine Specimen Smear Preparation

Answer 3

1. Label your slides.
2. Place a loopful of well-mixed urine and air dry.
3. Heat fix.

Question 4

List down the step-by-step procedure for Throat Swab Smear Preparation

Answer 4

1. Label your slides.
2. Collect specimen using sterile cotton swab.
3. Roll the swab gently back and forth.
4. Heat fix.

Question 5

Identify the Smear Preparation based on the step-by-step procedure listed:

1. Label your slides.
2. Place a loopful of NSS onto the slide.
3. Emulsify an isolate colony. Air dry.
4. Heat fix.

Answer 5

Plate Smear Preparation

Question 6

Identify the Smear Preparation based on the step-by-step procedure listed:

1. Label your slides.
2. Place a loopful of broth and air dry.
3. Heat fix.

Answer 6

Broth Smear Preparation

Question 7

Identify the Smear Preparation based on the step-by-step procedure listed:

1. Label your slides.
2. Place a loopful of well-mixed urine and air dry.
3. Heat fix.

Answer 7

Urine Specimen Smear Preparation

Question 8

Identify the Smear Preparation based on the step-by-step procedure listed:

1. Label your slides.
2. Collect specimen using sterile cotton swab.
3. Roll the swab gently back and forth.
4. Heat fix.

Answer 8

Throat Swab Smear Preparation

Question 9

List down the step-by-step procedure for Simple Staining

Answer 9

1. Flood the smear with Methylene Blue.
2. Set the timer for 1 Minute.
3. Remove the excess stain using gentle stream of water.
4. Blot dry and view under microscope.

Question 10

Preferred time for Simple Staining Method

Answer 10

1 Minute

Question 11

How to remove the excess stain in the slide with reagent?

Answer 11

Gentle stream of water is applied

Question 12

One of the stain used in Simple Staining Technique

Answer 12

Methylene Blue

Question 13

When labelling the slides in an actual laboratory setting, what information is written?

Answer 13

Name of the Patient
Date
Type of Specimen