(Laboratory) Microscopy Flashcards
Microscopy
1
Q
Micron (μ) and Millimicron (mμ) have been replaced by the terms _____ and _____
A
Micrometer (μm) and Nanometer (nm)
2
Q
_____ and _____ have been replaced by the terms micrometer (μm) and nanometer (nm)
A
Micron (μ) and Millimicron (mμ)
3
Q
Sizes of bacteria, fungi, and unicellular parasites are usually expressed in terms of _____
A
Micrometers
4
Q
Microbes are measured using an _____
A
Ocular Micrometer
5
Q
It is a tiny ruler within the eyepiece (ocular) of the compound light microscope
A
Ocular Micrometer
6
Q
1 meter is equivalent to _____ centimeters
A
100
7
Q
1 centimeter is equivalent to _____ millimeters
A
10
8
Q
1 millimeter is equivalent to _____ micrometer
A
1,000
9
Q
Approximate size of most viruses
A
0.01 to 0.3 micrometer
10
Q
Approximate size of Cocci bacteria
A
1 micrometer
11
Q
Approximate size of Bacilli bacteria
A
1 micrometer
12
Q
It is a spherical bacteria
A
Cocci
13
Q
Types of Bacteria according to its shape
A
Cocci
Bacilli
14
Q
Approximate size of Yeast
A
3 to 5 micrometer
15
Q
It is a rod-shaped bacteria
A
Bacilli
16
Q
Approximate size of a Septate hyphae
A
2 to 15 micrometer
17
Q
Approximate size of an Aseptate hyphae
A
10 to 30 micrometer
18
Q
A hyphae containing cross-walls
A
Septate hyphae
19
Q
A hyphae without cross-walls
A
Aseptate hyphae
20
Q
List down 3 Fungi:
A
Yeast
Septate hyphae
Aseptate hyphae
21
Q
Approximate size of Chlamydomonas
A
5 to 12 micrometer
22
Q
Approximate size of Euglena
A
35 to 55 micrometer
23
Q
Approximate size of Vorticella
A
50 to 145 micrometer
24
Q
Approximate size of Paramecium
A
180 to 300 micrometer
25
Approximate size of Volvoxa
350-500 micrometer
26
Approximate size of Stentor
1,000 to 2,000 micrometer
27
List down 6 types of Pond Water Protozoa
Chalmydomonas
Euglena
Vorticella
Paramecium
Volvoxa
Stentor
28
A microscope that has only one magnifying lens
Simple Microscope
29
This is an example of a Simple Microscope
Magnifying Glass
30
In Simple Microscopes, images appear about _____ larger than the object’s actual size
3 to 20 times
31
In Simple Microscope, images appear about 3 to 20 times (smaller or larger) than the object’s actual size
Larger
32
Leeuwenhoek’s simple microscopes had a maximum magnifying power of about _____
300 times
33
_____ simple microscopes had a maximum magnifying power of about X300 (300 times)
Leeuwenhoek’s
34
A microscope that contains more than one magnifying lens
Compound Microscope
35
Who are given credit for being the first to conceptualize Compound Microscopes?
Hans Jansen
Zacharias (Hans Jansen's Son)
36
Compound Microscopes magnify objects about _____
1,000 times
37
Photographs taken through the lens system of Compound Microscopes are called _____
Photomicrographs
38
Compound Microscopes is also referred to as _____
Compound Light Microscope
39
In Compound Microscopes, the wavelength of visible light is approximately _____ that limits the size of objects that can be seen
0.45 micrometer
40
Part of the Microscope that is located at the top of the microscope
Ocular Lens
41
Part of the Microscope that is located above the stage
Revolving Nosepiece
42
Part of the Microscope that is held in place above the stage by the revolving nosepiece
Objective Lenses
43
Part of the Microscope that is directly beneath the nosepiece and objective lenses
Stage
44
Part of the Microscope that is beneath the stage
Stage Adjustment Knobs
45
Part of the Microscope that is located on the condenser
Iris Diaphragm Control Arm
46
Part of the Microscope that is located beneath the stage
Condenser
47
Part of the Microscope that is located beneath the condenser
Collector Lens with Field Diaphragm
48
Part of the Microscope that is located on the front side of the base
Rheostat Control Knob
49
Part of the Microscope that is attached to the field diaphragm
Field Diaphragm Lever
50
Part of the Microscope that is on the side of the base
On and Off Switch
51
Part of the Microscope that is located beneath and behind the condenser
Condenser Control Knob
52
Part of the Microscope that is located on the arm of the microscope near the base
Fine and Coarse Adjustment Knobs
53
What is the part of the Microscope that holds the objective lenses?
Revolving Nose Piece
54
What is the part of the Microscope that is used to magnify objects placed on the stage?
Objective Lenses
55
What is the part of the Microscope that has a flat surface on which the specimen is placed for viewing?
Stage
56
What is the part of the Microscope that is used to move the stage and microscope slide?
Stage Adjustment Knobs
57
What is the part of the Microscope that is used to adjust the amount of light passing?
Iris Diaphragm Control Arm
58
What is the part of the Microscope that contains a lens system that focuses light onto the specimen?
Condenser
59
What is the part of the Microscope that controls the amount of light entering the condenser source?
Collector Lens with Field Diaphragm
60
What is the part of the Microscope that controls the amount of light emitted from the light?
Rheostat Control Knob
61
What is the part of the Microscope that is used to adjust the amount of light passing through the collector lens?
Field Diaphragm Lever
62
What is the part of the Microscope that is used to turn the light source on and off?
On and Off Switch
63
What is the part of the Microscope that contains the light source?
Base
64
What is the part of the Microscope that is used to adjust the height of the condenser?
Condenser Control Knob
65
What is the part of the Microscope that is used to focus the objective lenses?
Fine and Coarse Adjustment Knobs
66
What is the part of the Microscope that supports the binocular body and the revolving nosepiece; held with one hand when carrying the microscope, with the other hand beneath the base to support the weight of the Microscope?
Arm
67
What is the part of the Microscope that holds the ocular lenses in their proper locations?
Binocular Body
68
A microscope that is often used in the Clinical Laboratory
Brightfield Microscope
69
A microscope in which the objects are observed against a bright background
Brightfield Microscope
70
A microscope in which the objects are observed or seen against a dark background
Darkfield Microscope
71
This is a Microscope that is used in the diagnosis of Syphilis
Darkfield Microscope
72
In a Darkfield Microscope, the condenser is replaced with what is known as _____
Darkfield Condenser
73
Magnification of a Scanning Objective Lens
4x
74
Magnification of a Low Power Objective Lens
10x
75
Magnification of a High Power Objective Lens
40x
76
Magnification of an Oil Immersion Objective Lens
100x
77
A Microscope that is used to observe unstained living microorganisms
Phase-contrast Microscope
78
A Microscope that is often used in Immunology Laboratories
Fluorescence Microscope
79
Fluorescence Microscopes contains a built-in _____
Ultraviolet Light Source
80
A Microscope that is capable to view viruses
Electron Microscope
81
Electron Microscope uses an _____ as a source of illumination and magnets to focus the beam
Electron Beam
82
Electron Microscope uses and electron beam as a source of illumination and _____ to focus the beam
Magnets
83
The wavelength of electrons traveling in a vacuum is much (longer or shorter) than the wavelength of visible light
Shorter
84
Two types of Electron Microscope
Transmission Electron Microscopes
Scanning Electron Microscopes
85
A Microscope that is used to view the inside of your cell; inferior of the cell
Transmission Electron Microscope
86
In Transmission Electron Microscope, specimen must be very _____ and the electron beam will pass through the specimen
Thin
87
In Transmission Electron Microscope, electron gun fires a beam of electrons _____
Downward
88
A Microscope that is used to observe the outer surfaces of specimens
Scanning Electron Microscope
89
Scanning Electron Microscope's photographs are called _____
Scanning Electron Micrographs
90
Transmission Electron Microscope's photographs are called _____
Transmission Electron Micrographs
91
Most powerful type of Microscope
Atomic Force Microscope
92
A Microscope that observe living cells at extremely high magnification and resolution
Atomic Force Microscope
93
Formula for Total Magnification
Eyepice x Objective
