Lecture 3- Histology Flashcards
What can you see with low to intermediate magnification?
No individual cells
What can you see with high magnification
Cellular/ nuclear detail
What kind of magnification is this
Low
What kind of magnification is this
Intermediate
What kind of magnification is this
What is the diameter of a RBC
8 μm
What are the 3 types of microscopic imagery
Light microscopy, scanning electron microscopy, transmission electron microscopy
What does light microscopy use? It highlights what?
Photons, gross structure
What does scanning electron microscopy highlight?
Surface structure
What does Transmission electron microscopy highlight?
Internal cell structure
What produces this kind of image?
Light microscopy
What produces this kind of image?
Scanning electron microscopy
What produces this kind of image?
Transmission electron microscopy
What has the lowest resolution of microscopic imagery?
Light microscopy
With TEM what is a black structure
Electron dense
H&E stain relies on what
Electrostatic linkage between dyes and tissues
What is the most common stain
H&E
What stains dark blue/purple with H&E?
Components with a net negative charge (DNA/RNA/ glycosaminoglycans)are attracted to hematoxylin. Called Basophilic & stain blue
What stains pink with H&E stain
Components with a net positive charge (actin, myosin, collagen) attract Eosin. These are acidophilic and stain pink.
What is a limitation of H&E stain
Differentiating between collagen & muscle
What is the specialized collagen stain
Trichrome
What is Trichrome used to distinguish between
Collagen from muscle & epithelium
What is Trichrome used to diagnose
Scar tissue (liver cirrhosis) & smooth muscle tumors
With Trichrome stain what color does collagen stain? Muscle & epithelium? RBC? Nuclei?
Collagen: blue or green
Muscle & epithelium: red
Red blood cell: orange/red
Nuclei: brown/black
What test is ordered to look for carbohydrate rich areas?
Periodic acid- Schiff (PAS)
What is PAS used to diagnose
Mucus secreting tumors & basement membrane disorders
With a PAS stain what color do carbohydrate rich areas stain
Magenta
What test is ordered to diagnose fat tumors and fatty liver disease
Sudan red or osmic acid (black)
Sudan red or osmic acid (black) is used to highlight lipids in what cell types
Adipocytes & hepatocytes
What is a silver stain looking for
Damage to reticular fibers
Silver stain is used to diagnose damage to what
Filtration organs and basement membranes
A reticular fiber appears ______ with a silver stain
Branchy
Elastic fibers appear ____ with an orcein stain
Wavy
What is an Orcein stain looking for
Damage to elastic fibers
What can an Orcein stain diagnose
Inflammatory diseases of dermis and arteries that damage elastic fibers
Chemical stains highlight what
Classes of molecules but not a specific molecule
What does immunofluorescent staining do
Uses an antibody to tag a specific molecular target, then a dye is linked to the antibody
What kind of stain is this
Immunofluorescence
What produced this image?
Light microscopy
What produced this image?
Scanning electron microscopy
What produced this image?
Transmission electron microscopy
General base-like stains highlight what
Things with a net negative charge are called basophilic substances & they stain blue/ purple
General acid-like stains highlight what
Components with a net- positive charge which are called acidophilic substances & stain pink
What kind of stain made this image?
H&E
What kind of stain made this image?
Trichrome
What kind of stain made this image?
PAS
What kind of stain made this image?
Sudan red or osmic acid
What kind of stain made this image?
Silver
What kind of stain made this image?
Orcein
