Lecture 3- Histology

Question 1

What can you see with low to intermediate magnification?

Answer 1

No individual cells

Question 2

What can you see with high magnification

Answer 2

Cellular/ nuclear detail

Question 3

What kind of magnification is this

Answer 3

Low

Question 4

What kind of magnification is this

Answer 4

Intermediate

Question 5

What kind of magnification is this

Answer 5

Question 6

What is the diameter of a RBC

Answer 6

8 μm

Question 7

What are the 3 types of microscopic imagery

Answer 7

Light microscopy, scanning electron microscopy, transmission electron microscopy

Question 8

What does light microscopy use? It highlights what?

Answer 8

Photons, gross structure

Question 9

What does scanning electron microscopy highlight?

Answer 9

Surface structure

Question 10

What does Transmission electron microscopy highlight?

Answer 10

Internal cell structure

Question 11

What produces this kind of image?

Answer 11

Light microscopy

Question 12

What produces this kind of image?

Answer 12

Scanning electron microscopy

Question 13

What produces this kind of image?

Answer 13

Transmission electron microscopy

Question 14

What has the lowest resolution of microscopic imagery?

Answer 14

Light microscopy

Question 15

With TEM what is a black structure

Answer 15

Electron dense

Question 16

H&E stain relies on what

Answer 16

Electrostatic linkage between dyes and tissues

Question 17

What is the most common stain

Answer 17

H&E

Question 18

What stains dark blue/purple with H&E?

Answer 18

Components with a net negative charge (DNA/RNA/ glycosaminoglycans)are attracted to hematoxylin. Called Basophilic & stain blue

Question 19

What stains pink with H&E stain

Answer 19

Components with a net positive charge (actin, myosin, collagen) attract Eosin. These are acidophilic and stain pink.

Question 20

What is a limitation of H&E stain

Answer 20

Differentiating between collagen & muscle

Question 21

What is the specialized collagen stain

Answer 21

Trichrome

Question 22

What is Trichrome used to distinguish between

Answer 22

Collagen from muscle & epithelium

Question 23

What is Trichrome used to diagnose

Answer 23

Scar tissue (liver cirrhosis) & smooth muscle tumors

Question 24

With Trichrome stain what color does collagen stain? Muscle & epithelium? RBC? Nuclei?

Answer 24

Collagen: blue or green
Muscle & epithelium: red
Red blood cell: orange/red
Nuclei: brown/black

Question 25

What test is ordered to look for carbohydrate rich areas?

Answer 25

Periodic acid- Schiff (PAS)

Question 26

What is PAS used to diagnose

Answer 26

Mucus secreting tumors & basement membrane disorders

Question 27

With a PAS stain what color do carbohydrate rich areas stain

Answer 27

Magenta

Question 28

What test is ordered to diagnose fat tumors and fatty liver disease

Answer 28

Sudan red or osmic acid (black)

Question 29

Sudan red or osmic acid (black) is used to highlight lipids in what cell types

Answer 29

Adipocytes & hepatocytes

Question 30

What is a silver stain looking for

Answer 30

Damage to reticular fibers

Question 31

Silver stain is used to diagnose damage to what

Answer 31

Filtration organs and basement membranes

Question 32

A reticular fiber appears ______ with a silver stain

Answer 32

Branchy

Question 33

Elastic fibers appear ____ with an orcein stain

Answer 33

Wavy

Question 34

What is an Orcein stain looking for

Answer 34

Damage to elastic fibers

Question 35

What can an Orcein stain diagnose

Answer 35

Inflammatory diseases of dermis and arteries that damage elastic fibers

Question 36

Chemical stains highlight what

Answer 36

Classes of molecules but not a specific molecule

Question 37

What does immunofluorescent staining do

Answer 37

Uses an antibody to tag a specific molecular target, then a dye is linked to the antibody

Question 38

What kind of stain is this

Answer 38

Immunofluorescence

Question 39

What produced this image?

Answer 39

Light microscopy

Question 40

What produced this image?

Answer 40

Scanning electron microscopy

Question 41

What produced this image?

Answer 41

Transmission electron microscopy

Question 42

General base-like stains highlight what

Answer 42

Things with a net negative charge are called basophilic substances & they stain blue/ purple

Question 43

General acid-like stains highlight what

Answer 43

Components with a net- positive charge which are called acidophilic substances & stain pink

Question 44

What kind of stain made this image?

Answer 44

H&E

Question 45

What kind of stain made this image?

Answer 45

Trichrome

Question 46

What kind of stain made this image?

Answer 46

PAS

Question 47

What kind of stain made this image?

Answer 47

Sudan red or osmic acid

Question 48

What kind of stain made this image?

Answer 48

Silver

Question 49

What kind of stain made this image?

Answer 49

Orcein