Lecture 3 Flashcards
What is the cell theory? What are the components?
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How are tissues organised? What are the different layers? Diagram?
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What are epithelial cells?
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What are the different types of epithelial cells?
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What are squamous simple cells?
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What are squamous stratified cells?
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What are cuboidal simple cells?
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What are cuboidal stratified cells?
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What are columnar simple cells?
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What are columnar stratified cells?
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What are pseudostratified cells?
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What are transitional cells?
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What are the components of the cytoskeleton in epithelial cells? What are the properties? What are they made out of?
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What are the different types of cell junctions in epithelial cells? What are they made out of?
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What are Adherens junctions? What are the cell adhesion molecules?
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What are desmosomes? What are the cell adhesion molecules?
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What hemidesmosomes? What are the cell adhesion molecules?
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What is the table consisting of all the different types of cell junctions? What are the adhesion types? What are the cell adhesion molecules of each? What are the cytoskeletal attachment? What is the function?
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Why is cell architecture so important?
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What does mutations in desmoplakin result in?
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What is PPK? What are the symptoms? What are the treatment options? What is it caused by?
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How do mesenchymal cells connect?
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What is extracellular matrix?
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What are the main types of ECM abundant in all tissue?
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What are proteoglycans?
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What are fibrous ECM?
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What is multi-adhesive matrix proteins? f
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What is needed to permit the survival and function of cultured cells?
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What are the different stages of forming a cell culture?
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What is Dispase?
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How are mesenchymal cells added to the cell culture? What is needed?
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How are epithelial cells added into the cell culture? What is needed? What is the process?
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What are feeder layers? What is it used for?
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What are the focal adhesions in cell culture?
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What would happen to mesenchymal stem cells grown of substrates with varying stiffness?
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How does the stiffness of mesenchymal ECM affect stem cell lineage? Why?
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What are the limitations of cell culture in 2D? What are the differences to a 3D structure?
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What is an example of a 3D cell culture? How is it formed?
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What happens to the mammary epithelial cells once they formed a 3D culture rather than a 2D culture? What were the differences?
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What does the difference in 3D and 2D culture mean? Therefore what can you do?
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What are the three pillars of TERM?
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What is cells? What would be the source of cells?
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What is scaffolding? How does it affect the cell behaviour?
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What are the signals? How does it affect the cells?
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What is an Apilgraf?
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What are the three pillars of TERM to consider when engineering skin?
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What are the three pillars of TERM to consider when engineering a trachea?
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