Lecture 13 Flashcards
What are the different methods of potentially reprogramming a fibroblast into a cardiomyocyte?
dasdads
What is a master regulator? What are the master regulators of reprograming?
sadadsads
What were the candidate TFs which were come up with? What were their properties?
dwdasdasd
What is a reporter system? What was the reporter system used to identify CMs?
dasdasd
Were they able to directly programme fibroblasts into CMs? What was the problem?
edasdasd
What did they do to try and solve this problem?
dewsaads
What were the final 4 factors? What was the efficiency?
dasdasda
How did they narrow down the factors even more? What was the experiment?
dasdasd
What other genes are also cardiac specific?
dsadasd
What are iCMs?
dasdasd
What experiment has been done to show that the iCMs were actually CMs?
dasdasd
What showed that the iCMs has functional properties?
dsadasd
What showed whether the iCMs could integrate?
dsadasdasd
Can you reprogram fibroblasts in vivo? How? What was the experiment done?
dsadasdasd
What is the reporter system used to track the lineage of CM to see whether they came from CF?
dasdads
What were the results which showed that it was possible to reprogram in vivo CF –> CM? What showed it?
dasdasd
What were the properties of the reprogrammed cells? What did this show?
dwadsadsad
What about other cell types? What is the difference when looking at motor neurons?
dasdasdasd
How did they reprogram fibroblasts to neurons? What were the stages?
dasdasdasd
How did they pick the master regulators? What was the reporter system?
dasdasdads
How did they introduce the 19 TFs? What happened after introducing the TFs?
asdasdads
How did they narrow down the candidate TFs? How was this method different? What was the final combination?
dasdasdasd
What were the properties of the resulting iNeurons?
dasdasdasd
What cell cannot turn into iN?
dasdasdasd
Why can keratinocytes not be reprogrammed?
dasdasda
What is the trivalent histone state? What is the importance of it in reprogramming? What cells have it? What cells do not have it?
sqadasdasd
