Lecture 20 DNA virus REAL

Question 1

circular dsDNA

Answer 1

T antigen helicase nick fork
RNA primer bind
bidirectional replication
catenated decatenated topoisomerase

Question 2

what is the point of this lecture

Answer 2

mechanisms that replicate the genome (2 identical copies) that are packaged into the virion

Question 3

linear dsDNA

Answer 3

RNA primer removal = short 5’ ends (gaps)

susceptible to endonuclease

Question 4

concatemer linear dsDNA

Answer 4

SHRG joins short 5’ ends

endonuclease nicks into individual genome

Question 5

covalently closed ends

Answer 5

helicase nicks hairpin
3’ DNA pol primer
concatemer and cleaved

Question 6

circularisation

Answer 6

cohesive/blunt end ligation
helicase nicks, 3’ RPI (DNA primer)
RPD displaced, RNA primer bind
concatemer cleaved

Question 7

viral primer

Answer 7

early viral protein
bind 5’ viral DNA pol
synth strand displace other
“strand displacement replication”

Question 8

ssDNA circular

Answer 8

DNA pol convert dsDNA

rolling circle

Question 9

ssDNA linear

Answer 9

palindromic inverted repeat
hairpin 3' primer
flatten 
helicase nick and flatten (dsDNA intermediate)
3' hairpin bottom primer synthesis
displace upper strand fold genomic ssDNA