Lecture 2 - Staining Cont. and Cytology Flashcards
How many colors are in Hematoxylin - eosin (HE) stains?
Two (red and blue)
What stains red/pink in HE?
acidophilic, like cytoplasm
What stains blue/purple in HE?
Basophilic, like plasma cells, pancreatic exocrine cells, chief cells in stomach (arrows), neurons, chondroblasts, osteoblasts.
Essentially, cells that produce protein
Nucleus (DNA) and basal cytoplasm containing RER (RNA) are…
baso or acidophilic?
Basophilic
Apical cytoplasm with zymogen granules (enzyme precursors) – enzymes - proteins is... baso or acidophilic?
Acidophilic
What’s another word for acidophilic?
Eosinophilic
Silver stain is used for
reticular fibers, nerve fibers
Two types of elastic fiber stain?
Weigert’s elastic stain
Orcein stain
In silver stain, nerve fibers are what color?
Black
Was is Periodic Acid Schiff used for?
- stain for basement membrane and to localize carbohydrates (stain magenta)
- polysaccharides (carbohydrate polymers ) such as glycogen,
- mucosubstances such as glycoproteins, glycolipids and mucins in tissues
In a Pas stain, what color are goblet cells?
pink/purple
Goblet cells
secrete mucus to protect airways
How is lipid stain preparation different?
Don’t use alcohol-will wash out lipids
Instead, use frozen sections and can skip the washing and embedding steps
Enzyme histochemistry example:
In Gomori’s method for alkaline phosphatase the brush border of proximal convoluted tubules in the kidney stain what color?
black
Which colors bind to what in fluorescence microscopy?
Blue fluorescence binding to nuclear DNA.
Green fluorescent dye binds to actin filaments