Lecture 2 - Staining Cont. and Cytology Flashcards

1
Q

How many colors are in Hematoxylin - eosin (HE) stains?

A

Two (red and blue)

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2
Q

What stains red/pink in HE?

A

acidophilic, like cytoplasm

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3
Q

What stains blue/purple in HE?

A

Basophilic, like plasma cells, pancreatic exocrine cells, chief cells in stomach (arrows), neurons, chondroblasts, osteoblasts.
Essentially, cells that produce protein

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4
Q

Nucleus (DNA) and basal cytoplasm containing RER (RNA) are…
baso or acidophilic?

A

Basophilic

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5
Q
Apical cytoplasm with
zymogen granules (enzyme precursors) – enzymes - proteins is...
baso or acidophilic?
A

Acidophilic

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6
Q

What’s another word for acidophilic?

A

Eosinophilic

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7
Q

Silver stain is used for

A

reticular fibers, nerve fibers

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8
Q

Two types of elastic fiber stain?

A

Weigert’s elastic stain

Orcein stain

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9
Q

In silver stain, nerve fibers are what color?

A

Black

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10
Q

Was is Periodic Acid Schiff used for?

A
  • stain for basement membrane and to localize carbohydrates (stain magenta)
  • polysaccharides (carbohydrate polymers ) such as glycogen,
  • mucosubstances such as glycoproteins, glycolipids and mucins in tissues
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11
Q

In a Pas stain, what color are goblet cells?

A

pink/purple

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12
Q

Goblet cells

A

secrete mucus to protect airways

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13
Q

How is lipid stain preparation different?

A

Don’t use alcohol-will wash out lipids

Instead, use frozen sections and can skip the washing and embedding steps

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14
Q

Enzyme histochemistry example:
In Gomori’s method for alkaline phosphatase the brush border of proximal convoluted tubules in the kidney stain what color?

A

black

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15
Q

Which colors bind to what in fluorescence microscopy?

A

Blue fluorescence binding to nuclear DNA.

Green fluorescent dye binds to actin filaments

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16
Q

In polarized light microscopy, collagen fibers are what color?
What about elastic fibers and nuclei?

A

Bright red or yellow

Not detected

17
Q

Using an extra step to attach a secondary antibody (indirect), makes the compound

A

more specific

18
Q

Immunocytochemistry vs immunohistochemistry

A

Cyto - cell

Histo - tissue

19
Q

Three basic constituents of a cell

A
  • cytosol (hyaloplasm)
  • organelles (cell organelles)
  • cell inclusions (paraplasm)
20
Q

Hyaloplasm

A

basic structureless cytoplasm; the best electron microscopes reveal no structure in it.

21
Q

Cell organelles

A

the nucleus, mitochondria, the Golgi complex, ribosomes, the endoplasmic reticulum, lysosomes, the cytoskeleton (centriole, microtubules, actin and
myosin filaments, intermediate filaments), proteasomes.

22
Q

Cell inclusions (paraplasm)

A

secretory granules, glycogen, lipid droplets, crystaloids,

pigments: melanin, lipofuscin, lutein

23
Q

Multiple nuclei are present in what cells? (4)

A

skeletal muscle cells, osteoclasts, megakaryoblasts / megakaryocytes and in giant cells (found in pathological processes)

24
Q

In eukaryotic cells, the genome is separated from the cytoplasm by a

A

double nuclear envelope

25
Q

Is chromatin baso or acidophilic?

A

basophilic

26
Q

Two types of chromatin?

A

Heterochromatin (inactive) vs. Euchromatin (active)

27
Q

The nuclear envelope connects to

A

the RER

28
Q

What’s the purpose of nuclear pores?

A

allow passage of RNA, proteins into the cytoplasm

29
Q

What’s the purpose of the nucleolus?

A

to produce rRNA

30
Q

Most euchromatic cells fall into these four categories:

A
  • Neurons
  • Hepatocytes (liver cells)
  • Pancreatic acinar cells - digestion
  • Sertoli cells