Lecture 05

Question 1

3 function of blood

Answer 1

transport, maintain pH=7.3-7.45, protection

Question 2

4 things blood transport

Answer 2

nutrition
respiratory gas
hormone
temperature regulation

Question 3

experiment to understand component of blood & result

Answer 3

treat with anticoagulant, remain fluid
centrigue
3 different layer: plasma, buffy layer(WBC, platelet), RBC

Question 4

blood normal V, lower V, higher V

Answer 4

normovolemia, hypovolemia, hypervolemia

Question 5

hematocrit calculation& normal value

Answer 5

height of RBC coln/ height of whole blood coln, 45%

Question 6

4 composition of plasma

Answer 6

> 90% H2O

Na, Cl main ion

low quantity nutrition, respiratory gas, waste

protein

Question 7

blood, plasma, RBC V

Answer 7

5L, 2.75L, 2.25L

Question 8

3 blood protein & %

Answer 8

albumin(60%), globulin(35%), fibrinogen

Question 9

4 separate method of blood protein

Answer 9

differential precipitation by salt (eg. ammonium sulfaet)

sedimentation in ultracentrifuge b/c different MW

immunological characteristic b/c different cell surface protein

eletrophoresis

Question 10

4 step for electrophoresis

Answer 10

apply plasma

apply current

apply protein dye

see where these different bands have migrated

Question 11

result for electrophoresis

Answer 11

serum: fibrinogen removed
+, albumin, alpha 1 globulin, alpha 2 globulin, beta globulin, (fibrinogen removed in serum electrophoresis), gamma globulin

Question 12

origin of blood proteins

Answer 12

albumin, alpha 1 globulin, alpha 2 globulin, beta globulin, fibrinogen in liver

gamma globulin from lymphoid tissue

Question 13

electrophoresis in renal disease& reason

Answer 13

albumin much reduced, too much permeability at the level of renal tubule, albumin as the smallest plasma protein is able to flow out into urine

Question 14

electrophoresis in bacterial infection& reason

Answer 14

gamma globulin increase, fight infection by producing lots of AB

Question 15

body H2O compartment

Answer 15

body H2O: 60% body mass

intracellular fluid: 40% body mass, high in K

extracellular fluid: 20% body mass, high in Na, Cl

300mOsm=6.7atm~5100mmHg

interstitial fluid: 15% body mass, 10.5L
plasma: 5% body mass, 3.5L, more protein than interstitial fluid

Question 16

use of diffusion& starling forces

Answer 16

diffusion: exchange of nutrient, gas, waste across capillary wall

starling F: determine distribution of plasma& ISF

Question 17

2 starling F& function

Answer 17

colloidal osmotic/ oncotic pressure=25mmHg

pull in fluid from inside capillary

filtration: bulk flow across a porous membrane which act as a sieve, withholding some particles

b/c hydrostatic P from heart beat, push out from inside capillary into ISF