Lec 07 Microbiology of Selected Bacteria and Viruses

Question 1

MacConkey agar is a selective media because it targets only G+ and recovers G- bacteria thru the presence of:

Answer 1

Bile salts

Question 2

O/H/K Antigen?

The somatic antigen in cell wall LPS

Answer 2

O Antigen

Question 3

O/H/K Antigen?

Flagella proteins

Answer 3

H Antigen

Question 4

Most common presentation of Enterobacteriaciae infection

Answer 4

diarrhea

Question 5

process by which DNA is transferred from one bacterium to another by a virus

Answer 5

Transduction

Question 6

Appearance of Salmonella in Hektoen culture media

Answer 6

Green/blue colonies with black centers

Question 7

4F’s of Food Safety

Answer 7

Fingers
Feces
Flies
Foot

Flo

Question 8

Probably the world’s most widespread zoonosis.

Answer 8

Leptospirosis

Question 9

Temperature and PH for optimal growth of Leptospira

Answer 9

Temp: 28-32C, PH 7.2-7.6

Question 10

True of leptospires:

a. Uses long chain fatty acids as source of energy
b. Organic matter for nitrogen source
c. Both
d. Neither

Answer 10

a.

They can process amino acids or carbohydrates as nitrogen source. Just ammonium salts. So b is false.

Question 11

Incubation period for Leptospira

Answer 11

1-2 weeks

Question 12

most common clinical manifestations of leptospirosis

Answer 12

jaundice and conjunctival suffusion

Question 13

True of laboratory diagnosis of leptospirosis:

a. urine specimen on the 3rd weeks inoculated unto 
liquid medium
b. IgM detected at the end of 1st week
c. Both
d. Neither

Answer 13

d. Neither
Urine specimen gathered at 2-6 weeks sa semisolid medium + neomycin sulfate
Yung IgM at end of first week, parang sa typhoid yun.

Question 14

substance injected to animal to create resistance to the disease

Answer 14

inoculum

Question 15

A result of 2+ for Leptodipstick assay indicates:

Answer 15

that there is evidence of recent leptospirosis.

1+ = assay should be taken at a later time/ re-examination to look for an increased amount of antibodies.

Question 16

Viruses transmitted by blood-sucking athropods from one

host to another during the viremic stage.

Answer 16

Arbovirus (ARthropod-BOrne) viruses

Question 17

True of Arthropod borne viruses:
A. Blood sucking arthropods are the vectors particularly
ticks
B. The vectors are infected throughout their lives without
signs of damage
C. Both
D. Neither

Answer 17

B.

Mosquitoes can be vectors.

Question 18

Swahili for “Break-bone fever”

Answer 18

Dengue

Question 19

3 protein molecules that form the Flaviviridae virus particle:

Answer 19

C, prM, E

Question 20

Grade of DHF:

Spontaneous bleeding into the skin and elsewhere.

Answer 20

Grade II

Grade I
- Bruising and (+) torniquet test in persons
with fever (20 or more petechiae per 1 inch2)
Grade II
- Spontaneous bleeding into the skin and elsewhere
Grade III
- Clinical evidence of shock
Grade IV
- Severe shock: blood pressure and pulse cannot be detected

o Note: Grades III & IV = ―Dengue

Syndrome (DSS)