Lec 06 Principles of Diagnostic Microbiology

Question 1

growth of SAME organism in repeated cultures obtained at different sites and time
A. Bacteremia
B. Contamination

Answer 1

A. Bacteremia

Question 2

growth of different organism in different cultures obtained at different sites and time
A. Bacteremia
B. Contamination

Answer 2

B. Contamination

Question 3

definition of good sputum specimen

Answer 3

minimal epithelial cells 5

Question 4

When should you collect sputum?

Answer 4

morning

Question 5

Used for the transport of Anaerobes

Answer 5

Deep Semi-solid thioglycolate

Question 6

Transport medium for Typhoid bacilli

Answer 6

Glycerol Saline

Question 7

What do you do to a stool sample when you expect a delay in transport?

Answer 7

Add about 6 mL buffered glycerol saline transport medium

Question 8

T or F. Endocervical swabs are used to collect trichomoniasis in females

Answer 8

False. It is only used for gonococci

Question 9

Type of medium. Antibiotics, chemicals added to allow selective growth

Answer 9

Selective media

Question 10

Type of medium. May grow more than one type of organism, but the composition of the media and growth characteristics of microorganism allow differentiation

Answer 10

Differential media

Question 11

the concentration of antimicrobial that

kills all bacteria

Answer 11

Minimum Bacteriocidal Concentration

Question 12

Developed in Sweden for direct quantification of antimicrobial susceptibility using the agar diffusion method

Answer 12

E-test

Question 13

Virus that causes a cell fusion cytopathic effect

Answer 13

Measles

Question 14

Virus that causes acidophilic inclusion bodies in the nucleus

Answer 14

Papovavirus

Question 15

Stain used for Curvularia sp.

Answer 15

Lactophenol blue