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BACT 211 > Laboratory Diagnosis of Enterobacteriaceae > Flashcards

Laboratory Diagnosis of Enterobacteriaceae Flashcards

1
Q

Yersinia spp. bipolar appearance, closed safety pin appearance.

A

Wayson stain or Methylene Blue stain

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2
Q

Blue rod with prominent polar granules, giving rise to the safety-pin appearance,surrounded by a large, pink capsule.

[microscopic examination]

A

Donovan Body

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3
Q

Used as enrichment for the isolation of Shigella and other Enterobacteriaceae.

[culture media]

A

Gram-negative broth (GN) or Selenite broth (SEL)

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4
Q

Medium specifically used for the isolation of Y. enterocolitica from gastrointestinal specimens.

[culture media]

A

Cefsulodin-irgasan-novobiocin (CIN) agar

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5
Q

It is used to differentiate sorbitolnegative E. coli O157:H7 from other strains of E. coli that are capable of fermenting this sugar alcohol.

[culture media]

A

MacConkeysorbitol agar (MAC-SOR)

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6
Q

Broth cultures of Y. pestis exhibit a characteristic ____ in which clumps of cells adhere to one side of the tube.

A

Stalactite pattern

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7
Q

Colonies of Y. pestis on 5% sheep blood agar are ____ at 24 hours but exhibit a ___, ____ appearance at 48 hours.

A

pinpoint, rough, cauliflower

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8
Q

Production of hydrogen sulfide (H2S); ferrous sulfate. Salmonella spp. Positive reactions appear as brown to black precipitate.

[isolation of salmonella spp. and shigella spp.]

A

Bismuth sulfite agar

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9
Q

Positive reactions of Bimuth Sulfite Agar.

[color]

A

brown to black

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10
Q

Salmonella spp. appear as white to pink or red colonies surrounded by a bright halo.

[isolation of salmonella spp. and shigella spp.]

A

Brilliant green agar

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11
Q

Appear colorless.

[shigella, salmonella]

A

Shigella spp.

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12
Q

Colorless with a black center.

[shigella, salmonella]

A

Salmonella spp.

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13
Q

Selective enrichment for the growth of Salmonella spp.

A

Selenite broth

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14
Q

Colorless to red with black center. (Xylose-lysinedeoxychocolate agar)

[shigella, salmonella]

A

Salmonella spp.

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15
Q

Colorless to red. (Xylose-lysinedeoxychocolate agar)

[shigella, salmonella]

A

Shigella spp.

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16
Q

Lactose fermenter, forms blueblack with a metallic green sheen.

[Eosin/Methylene blue agar (EMB]

A

Escherichia coli

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17
Q

form pink colonies.

[Eosin/Methylene blue agar (EMB]

A

Other coliform fermenters

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18
Q

Translucent, either amber or colorless.

[Eosin/Methylene blue agar (EMB]

A

Nonfermenters

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19
Q

TSI means

A

Triple Sugar Iron agar

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20
Q

[3] Sugar in TSI agar

A

Glucose
Lactose
Sucrose

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21
Q

acidic (yellow) resulting from the fermentation of glucose under anaerobic conditions.

[TSI]

A

Butt

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22
Q

lactose and/or sucrose are fermented:

[TSI]

A

Siant

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23
Q

[3] Quality Control

A
  1. A/A
  2. K/A H2S+
  3. K/NC
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24
Q

A/A

[quality control]

A

Escherichia coli

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25
Q

K/A H2S+

[quality control]

A

Salmonella typhi

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26
Q

K/NC:

[quality control]

A

Pseudomonas aeruginosa

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27
Q

Drawing a circle around the A for the acid butt, that is, A/A , usually indicates production of ____?

A

Gas

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28
Q

Indicate the production of CO2 and H2 in the tube.

A

Bubbles or cracks

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29
Q

Contains lysine, peptones, a small amount of glucose, ferric ammonium citrate, and sodium thiosulfate.

A

Lysin Iron Agar

30
Q

When glucose is fermented, the butt of the medium becomes ___?

A

Acidic (yellow)

31
Q

What forms if the organism produces lysine decarboxylase?

A

Cadaverine

32
Q

It neutralizes the organic acids formed by glucose fermentation, and the butt of the medium reverts to the alkaline state (purple)

A

Cadaverine

33
Q

Organism decarboxylates but cannot deaminate, ferments glucose, first butt is yellow then turns back to purple.

[LIA]

34
Q

Organism fermented glucose but was unable to deaminate or decarboxylate lysine.

[LIA]

35
Q

Organism deaminated lysine but could not decarboxylate it.

[LIA]

36
Q

Lysine decarboxylation and no fermentation of glucose.

[LIA result]

A

Alkaline slant/ Alkaline butt (K/K)

37
Q

Glucose fermentation

[LIA result]

A

Alkaline slant/acid butt (K/A)

38
Q

Lysine deamination and glucose fermentation.

[LIA result]

A

Red slant/acid butt (R/A)

39
Q

Alkaline slant and butt, H2S positive:

[LIA, quality control]

A

Salmonella typhimurium

40
Q

Alkaline slant, acid butt:

[LIA, quality control]

A

Shigella flexneri

41
Q

Red slant, acid butt:

[LIA, quality control]

A

Proteus vulgaris

42
Q

Determines whether an organism possesses the enzyme that deaminates phenylalanine to phenylpyruvic acid.

[test]

A

Phenylalanine Deaminase (PAD)

43
Q

This test is helpful in initial differentiation of Proteus, Morganella, and Providencia organisms, which are positive, from the rest of the Enterobacteriaceae.

A

Phenylalanine Deaminase (PAD)

44
Q

A semisolid agar.

A

Sulfide indole motility

45
Q

Color that indicates the production of H2S.

[SIM agar]

A

Black precipitate

46
Q

Color that indicated the addition of Kovac’s reagent is positive for indole.

[SIM agar]

A

pink to red

47
Q

It is one of the degradation products of the amino acid tryptophan

[SIM agar]

48
Q

What color that indicates that indole is present.

[SIM agar]

49
Q

In SIM agar medium, it can be utilized by bacteria, with the production of hydrogen sulfide.

A

Sodium thiosulfate

50
Q

What color is H2S in SIM agar.

51
Q

It is more sensitive than Kovac’s reagent and is preferred with nonfermentative bacteria.

[method]

A

Ehrlich method

52
Q

Color that indicate that indole is present after the addition of PDAB.

53
Q

This test is used to determine the ability of an organism to utilize sodium citrate as its only carbon source and inorganic ammonium salts as its only nitrogen source.

[test]

A

Citrate utilization

54
Q

Color of bacteria that can grown on citrate utilization.

A

Bromothymol blue

55
Q

Bromothymol Blue is GREEN.

[pH]

56
Q

Bromothymol BLUE

[pH]

57
Q

Quality control: Positive

[Citrate utilization]

A

Klebsiella pneumoniae

58
Q

Quality control: Negative

[Citrate utilization]

A

Escherichia coli

59
Q

Alternative medium of Citrate Utilization.

A

Christensen’s citrate

60
Q

This medium incorporates phenol red (as the pH indicator) and organic nitrogen.

A

Christensen’s citrate medium

61
Q

This test is used to determine the ability of an organism to produce the enzyme urease, which hydrolyzes urea.

A

Urea hydrolysis (Christensen’s method)

62
Q

[2] Hydrolysis of urea produces.

[A,C]

A

Ammonia and CO2

63
Q

Expected results of Urea Hydrolysis if NEGATIVE.

A

No color change

64
Q

Expected results of Urea Hydrolysis if POSITIVE.

A

Light orange to magenta

65
Q

Quality control of Urea Hydrolysis: POSITIVE

A

Proteus vulgaris

66
Q

Quality control of Urea Hydrolysis: NEGATIVE

A

Escherichia coli

67
Q

Quality control of Methyl Red: POSITIVE

A

Escherichia coli

68
Q

Quality control of Methyl Red: NEGATIVE

A

Enterobacter cloacae

69
Q

Quality control of B. VP (Barritt’s method): POSITIVE

A

Enterobacter cloacae

70
Q

Quality control of B. VP (Barritt’s method): NEGATIVE

A

Escherichia coli

71
Q

This test determines whether an organism has the ability to reduce nitrate to nitrite and reduce nitrite further to nitrogen gas (N2).

A

Nitrate Reduction Test

72
Q

Color that indicates of presence of nitrite.

BACT 211 (14 decks)

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