Specimen Collection & Processing

Question 1

Collected specimen in wounds or lesions is placed into what?

Answer 1

sterile tube or transport vial

Question 2

Blood culture for wounds.

Answer 2

Alcohol and Iodine

Question 3

[2] Blood culture set for wounds.

Answer 3

Aerobic and anaerobic

Question 4

Collected amount of wound specimen for adult.

Answer 4

20 ml

Question 5

Collected amount of wound specimen for children.

Answer 5

5-10 ml

Question 6

3 specimens in 3 consecutive days.

[infection]

Answer 6

Bacterial infection

Question 7

3 samples within 10 days.

[infection]

Answer 7

Parasitic infection

Question 8

It appears as a white chalky substance in the specimen and masks the appearance of parasites under the microscope.

Answer 8

Barium

Question 9

Some specimens, such as urine, stool, sputum, swabs (not for anaerobes), foreign devices such as catheters, and viral specimens can be maintained at refrigerator temperature.

[temperature]

Answer 9

4° C for 24 hours.

Question 10

If cerebrospinal fluid is not processed immediately, it can be stored in what temperature?

Answer 10

35° C incubator for 6 hours

Question 11

[7] Refrigerate

(CCE,FFSU)

Answer 11

1. Catheter tips (IV)
2. CSF for viruses
3. Ear: outer
4. Feces (unpreserved)
5. Feces for Clostridium difficile toxin
6. Sputum
7. Urine (unpreserved)

Question 12

[9] Room temperature

(ABCEFG, NTU)

Answer 12

1. Abscess, lesion, wound
2. Body fluids
3. CSF for bacteria
4. Ear: inner
5. Feces (preserved)
6. Genital
7. Nasal
8. Tissue
9. Urine (preserved)

Question 13

It is used in commercial products to maintain accurate urine colony counts.

Answer 13

Boric acid

Question 14

If the delay is longer than 2 hours, the specimen can be added to.

Answer 14

Cary-Blair transport media

Question 15

Used to prevent clotting of specimens, including blood, bone marrow, and synovial fluid.

Answer 15

Anticoagulants

Question 16

The most common anticoagulant used for microbiology specimens.

Answer 16

Sodium Polyanethol Sulfonate (SPS)

Question 17

Used for viral cultures and for isolation of Mycobacterium spp. from blood.

Answer 17

Heparin

Question 18

[2] It should not be used for microbiology specimens.

Answer 18

1. Citrate
2. Ethylenediamine Tetra acetic Acid (EDTA)

Question 19

These media usually contain substances that do not promote multiplication of microorganisms but ensure their preservation and are available in swab collection systems.

Answer 19

Holding or Transport Media

Question 20

It is commonly used.

[holding or transport media]

Answer 20

Stuart’s or Amie’s transport medium

Question 21

This system contains selective agar and a carbon dioxide (CO2)–generating tablet.

Answer 21

JEMBEC system

Question 22

The growth of most non-fastidious microbes.

[culture media]

Answer 22

Nonselective media

Question 23

Support the growth of one type or group of microbes but not another.

[culture media]

Answer 23

Selective media

Question 24

The standard nonselective medium.

[culture media]

Answer 24

Sheep blood agar

Question 25

It's an enriched medium.

Answer 25

Chocolate agar

Question 26

It is selective for enteric gram-negative bacilli, and CNA (Columbia agar with colistin and nalidixic acid) is selective for gram, [culture media]

Answer 26

MacConkey agar

Question 27

Allow grouping of microbes based on different characteristics demonstrated on the medium. [culture media]

Answer 27

Differential media

Question 28

It can be used as a supplement to agar plates to detect small numbers of most aerobes, anaerobes, and microaerophiles. [broth]

Answer 28

Broth media

Question 29

It is an example of a supplemental broth media. [broth]

Answer 29

Thioglycollate broth (THIO)

Question 30

It is used to enhance the growth of group B streptococci. [broth]

Answer 30

Lim broth (Todd Hewitt with CNA)

Question 31

Urine specimens are inoculated using a?

Answer 31

quantitative isolation

Question 32

Most bacteria cultures are incubated. [temp]

Answer 32

35° C to 37° C.

Question 33

The swab is immersed in the specimen for several seconds and used to prepare a thin spread of material on the glass slide fo staining and viewing.

Answer 33

Smears from thick liquids or semisolids

Question 34

[3] Categorization of Stains

Answer 34

1. Simple stain 2. Differential stain 3. Pro-mediated stains

Question 35

It is directed toward coloring the forms and shapes present. [stains]

Answer 35

Simple stains

Question 36

It is directed toward coloring specific components of the elements present. [stains]

Answer 36

Differential stains

Question 37

It is directed specifically at identification of an organism. [stains]

Answer 37

Pro-mediated stains

Question 38

[4] Four of the stains. [GACR]

Answer 38

1. Gram 2. Acid-fast 3. Calcofluor white 4. Rapid Modified Wright-Giemsa

Question 39

It was developed empirically by the Danish bacteriologist Christian Gram in 1884.

Answer 39

Gram stain

Question 40

Bacteriologist who developed Gram Stain.

Answer 40

Christian Gram, 1884

Question 41

[3] Pairs: Gram positive cocci

Answer 41

1. Staphylococcus 2. Streptococcus 3. Enterococcus

Question 42

[3] Tetrads: Gram positive cocci

Answer 42

1. Micrococcus 2. Staphylococcus 3. Peptostreptoccus

Question 43

[3] Groups/Clusters: Gram positive cocci

Answer 43

1. Staphylococcus 2. Peptostreptococcus 3. Stomatococcus

Question 44

[2] Chains: Gram positive cocci

Answer 44

1. Streptococcus 2. Peptostreptococcus

Question 45

[3] Encapsulated: Gram positive cocci [PPM]

Answer 45

1. Streptococcus pneumoniae 2. Streptococcus pyogenes 3. Streptococcus mucilaginosus

Question 46

[1] Gram-positive diplococci (lancet-shaped)

Answer 46

Streptococcus pneumoniae

Question 47

[2] Gram--negative diplococci pathogenic

Answer 47

1. Neisseria spp. 2. Moraxella catarhallis