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BACT 211 > Specimen Collection & Processing > Flashcards

Specimen Collection & Processing Flashcards

1
Q

Collected specimen in wounds or lesions is placed into what?

A

sterile tube or transport vial

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2
Q

Blood culture for wounds.

A

Alcohol and Iodine

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3
Q

[2] Blood culture set for wounds.

A

Aerobic and anaerobic

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4
Q

Collected amount of wound specimen for adult.

A

20 ml

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5
Q

Collected amount of wound specimen for children.

A

5-10 ml

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6
Q

3 specimens in 3 consecutive days.

[infection]

A

Bacterial infection

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7
Q

3 samples within 10 days.

[infection]

A

Parasitic infection

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8
Q

It appears as a white chalky substance in the specimen and masks the appearance of parasites under the microscope.

A

Barium

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9
Q

Some specimens, such as urine, stool, sputum, swabs (not for anaerobes), foreign devices such as catheters, and viral specimens can be maintained at refrigerator temperature.

[temperature]

A

4° C for 24 hours.

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10
Q

If cerebrospinal fluid is not processed immediately, it can be stored in what temperature?

A

35° C incubator for 6 hours

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11
Q

[7] Refrigerate

(CCE,FFSU)

A
  1. Catheter tips (IV)
  2. CSF for viruses
  3. Ear: outer
  4. Feces (unpreserved)
  5. Feces for Clostridium difficile toxin
  6. Sputum
  7. Urine (unpreserved)
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12
Q

[9] Room temperature

(ABCEFG, NTU)

A
  1. Abscess, lesion, wound
  2. Body fluids
  3. CSF for bacteria
  4. Ear: inner
  5. Feces (preserved)
  6. Genital
  7. Nasal
  8. Tissue
  9. Urine (preserved)
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13
Q

It is used in commercial products to maintain accurate urine colony counts.

A

Boric acid

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14
Q

If the delay is longer than 2 hours, the specimen can be added to.

A

Cary-Blair transport media

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15
Q

Used to prevent clotting of specimens, including blood, bone marrow, and synovial fluid.

A

Anticoagulants

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16
Q

The most common anticoagulant used for microbiology specimens.

A

Sodium Polyanethol Sulfonate (SPS)

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17
Q

Used for viral cultures and for isolation of Mycobacterium spp. from blood.

A

Heparin

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18
Q

[2] It should not be used for microbiology specimens.

A
  1. Citrate
  2. Ethylenediamine Tetra acetic Acid (EDTA)
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19
Q

These media usually contain substances that do not promote multiplication of microorganisms but ensure their preservation and are available in swab collection systems.

A

Holding or Transport Media

20
Q

It is commonly used.

[holding or transport media]

A

Stuart’s or Amie’s transport medium

21
Q

This system contains selective agar and a carbon dioxide (CO2)–generating tablet.

A

JEMBEC system

22
Q

The growth of most non-fastidious microbes.

[culture media]

A

Nonselective media

23
Q

Support the growth of one type or group of microbes but not another.

[culture media]

A

Selective media

24
Q

The standard nonselective medium.

[culture media]

A

Sheep blood agar

25
Q

It’s an enriched medium.

A

Chocolate agar

26
Q

It is selective for enteric gram-negative bacilli, and CNA (Columbia agar with colistin and nalidixic acid) is selective for gram,

[culture media]

A

MacConkey agar

27
Q

Allow grouping of microbes based on different characteristics demonstrated on the medium.

[culture media]

A

Differential media

28
Q

It can be used as a supplement to agar plates to detect small numbers of most aerobes, anaerobes, and microaerophiles.

[broth]

A

Broth media

29
Q

It is an example of a supplemental broth media.

[broth]

A

Thioglycollate broth (THIO)

30
Q

It is used to enhance the growth of group B streptococci.

[broth]

A

Lim broth (Todd Hewitt with CNA)

31
Q

Urine specimens are inoculated using a?

A

quantitative isolation

32
Q

Most bacteria cultures are incubated.

[temp]

A

35° C to 37° C.

33
Q

The swab is immersed in the specimen for several seconds and used to prepare a thin spread of material on the glass slide fo staining and viewing.

A

Smears from thick liquids or semisolids

34
Q

[3] Categorization of Stains

A
  1. Simple stain
  2. Differential stain
  3. Pro-mediated stains
35
Q

It is directed toward coloring the forms and shapes present.

[stains]

A

Simple stains

36
Q

It is directed toward coloring specific components of the elements present.

[stains]

A

Differential stains

37
Q

It is directed specifically at identification of an organism.

[stains]

A

Pro-mediated stains

38
Q

[4] Four of the stains.

[GACR]

A
  1. Gram
  2. Acid-fast
  3. Calcofluor white
  4. Rapid Modified Wright-Giemsa
39
Q

It was developed empirically by the Danish bacteriologist Christian Gram in 1884.

A

Gram stain

40
Q

Bacteriologist who developed Gram Stain.

A

Christian Gram, 1884

41
Q

[3] Pairs: Gram positive cocci

A
  1. Staphylococcus
  2. Streptococcus
  3. Enterococcus
42
Q

[3] Tetrads: Gram positive cocci

A
  1. Micrococcus
  2. Staphylococcus
  3. Peptostreptoccus
43
Q

[3] Groups/Clusters: Gram positive cocci

A
  1. Staphylococcus
  2. Peptostreptococcus
  3. Stomatococcus
44
Q

[2] Chains: Gram positive cocci

A
  1. Streptococcus
  2. Peptostreptococcus
45
Q

[3] Encapsulated: Gram positive cocci

[PPM]

A
  1. Streptococcus pneumoniae
  2. Streptococcus pyogenes
  3. Streptococcus mucilaginosus
46
Q

[1] Gram-positive diplococci (lancet-shaped)

A

Streptococcus pneumoniae

47
Q

[2] Gram–negative diplococci pathogenic

A
  1. Neisseria spp.
  2. Moraxella catarhallis

BACT 211 (9 decks)

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