Aerobic Gram-Positive Bacilli Flashcards

1
Q

[2] species a Corynebacteria can be divided

A
  1. Nonlipopholic
  2. Lipophilic
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2
Q

“club shape”

A

Corynebacterium

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3
Q

Cells are arranged singly, in “palisades” of parallel cells.

A

Corynebacterium

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4
Q

or in pairs of cells connected after cell division to form V or L shapes: “Chinese letters” appearance.

A

Corynebacterium

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5
Q

diphtheroid, means

A

“diphtheria like”

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6
Q

Disease caused by C. diphtheriae is referred to as?

A

diptheria

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7
Q

Club-shaped swellings and beaded forms are common.

[corynebacterium]

A

Corynebacterium diphtheriae

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8
Q

The metachromatic areas of the cell, which stain more intensely than other parts, are called?

[c.diphtheriae]

A

Babès-Ernst granules.

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9
Q

They represent accumulation of polymerized polyphosphates.

[corynebacterium]

A

Corynebacterium diphtheriae

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10
Q

Typical coryneform gram-positive rods with “jointed ends” giving L and V forms, and coccoid cells after 72 hr (i.e., rod-coccus cycle*).

A

Arthrobacter spp.

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11
Q

rod-coccus cycle

A

Brevibacterium spp.

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12
Q

Irregular, short, thin, branching, gram positive rods.

A

Cellulomonas spp.

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13
Q

Better growth is usually obtained on a medium containing blood or serum, such as Loeffler serum or Pai agars.

[corynebacterium]

A

C. diphtheriae

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14
Q

A modification of Tinsdale medium, contains sheep red blood cells, bovine serum, cystine, and potassium tellurite.

[agar]

A

Cystine-tellurite blood agar

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15
Q

brown-black colonies with a gray-brown halo

[agar]

A

Tinsdale agar

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16
Q

cheese-like odor on BAP

A

Brevibacterium spp.

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17
Q

Corynebacteria form black or brownish colonies from the reduction of tellurite. (brown halo)

[agar]

A

Cystine-tellurite blood agar (CTBA)

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18
Q

Stimulates the growth of C. diphtheriae and the production of metachromatic granules in the cells. Produces gray to white, translucent colonies.

[medium]

A

Loeffler medium

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19
Q

[3] Corynebacterium that form a BROWN HALO.

A
  1. C. diphtheriae
  2. C. ulcerans
  3. C. pseudotuberculosis
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20
Q

Distinguished from the other two species by its lack of urease production.

[corynebacterium]

A

C. diphtheriae

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21
Q

Ferments glucose and maltose, producing acid but not gas, and reduces nitrate to nitrite.

[corynebacterium]

A

C. diphtheriae

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22
Q

Organisms (controls and unknowns) are streaked on medium of low iron content.

[test]

A

Elek test

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23
Q

It is often associated with PROSTETIC JOINT infection and has been reported to cause bloodstream infection and endocarditis.

[corynebacterium]

A

Corynebacterium amycolatum

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24
Q

The most common cause of Corynebacterium associated prosthetic valve endocarditis in adults.

[corynebacterium]

A

Corynebacterium jeikeium

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25
The organism is LIPOPHILIC and a STRICT AEROBE that is nonhemolytic, does not produce urease, and is nitrate-reduction negative. [corynebacterium]
Corynebacterium jekeium
26
It has been isolated from skin ulcers and exudative pharyngitis. [corynebacterium]
Corynebacterium ulcerans
27
Mastitis in cattle and other domestic and wil animals. [corynebacterium]
Corynebacterium ulcerans
28
Most commonly associated with UTIs. [corynebacterium]
Corynebacterium urealyticum
29
Nitrate-negative, catalase-positive, and RAPIDLY UREASE POSITIVE within minutes after inoculation on a Christensen urea slant. [corynebacterium]
Corynebacterium urealyticum
30
It has been used to reliably identify toxigenic strains of Corynebacterium spp. [MALDI - TOF MS]
Matrix-assisted laser desorption ionization time of light mass spectroscopy.
31
Survival within a phagocyte. [listeria monocytogenes]
Listeriolysin O
32
Ingestion of contaminated food, such as MEAT and DAIRY PRODUCTS.
Listeria Monocytogenes
33
May colonize human fetus.
Listeria Monocytogenes
34
Central nervous system (CNS) [clinical infections]
Listeria monocytogenes
35
Responsible for spontaneous abortion and stillborn neonates.
Listera monocytogenes
36
L. monocytogenes grows at __° C, a technique called ______?
4° C cold enrichment
37
In wet mount preparations, it exhibits "tumbling motility" (end-over-end motility) when viewed microscopically.
Listeria monocytogenes
38
In motility medium, the characteristic “umbrella” pattern is seen when the organism is incubated at room temperature (22° C to 25°C) but not at 35° C.
Listeria monocytogenes
39
Produces a “block” type hemolysis with the CAMP test.
L. monocytogenes
40
Antibodies can be detected in cases of listeriosis.
Antilisteriolysin O IgG
41
Gram-positive cocci that can appear rodlike that belong to the family Micrococcaceae.
Rothia spp.
42
Bacteremia, endocarditis, pneumonia, and other infections. [rothia]
Rothia mucilaginosa
43
Normal human oropharyngeal microbiota. [rothia]
Rothia dentocariosa
44
It is a gram-positive, gram variable, catalase negative, non spore-forming, pleomorphic rod that has a tendency to form long filaments.
Erysipelothrix rhusiopathiae
45
Arranged singly, in short chains, or in a V shape
Erysipelothrix rhusiopathiae
46
Involves handling fish and animal products.
Erysipelothrix rhusiopathiae
47
[4] Virulence factors or Erysipelothrix rhusiopathiae
1. Capsule 2. Neuranminidase 3. Hyaluronidase 4. Surface proteins
48
Growth of _______ in a gelatin stab culture yields a highly characteristic “test tube brush like” pattern at 22° C..
E. rhusiopathiae
49
Treatment for Erysipelothrix rhusiopathiae.
Penicillin
50
Association with bacterial vaginosis (BV) in humans.
Gardnerella vaginalis
51
Medium of choice for G. vaginalis.
Human blood bilayer tween (HBT) agar
52
Normal microbiota: Human mouth, gastrointestinal tract, and female genital tract. [spp.]
Lactobacillus spp.
53
Important for maintaining the proper pH balance in vaginal secretions. [spp.]
Lactobacillus spp.
54
The organism enters the bloodstream during chewing, brushing teeth, and dental procedures, resulting in bacteremia and endocarditis. [spp.]
Lactobacillus spp.
55
Growth on Tomato Juice Agar [spp.]
Lactobacillus spp.
56
Only Arcanobacterium spp. that has been recovered from clinica; specimens.
A. haemolyticum
57
[2] Family Nocardiaceae
1. Nocardia 2. Rhodococcus
58
[3] Nocardia spp. cause three types of skin infections in immunocompetent individuals:
1. Mycetoma 2. Lymphocutaneous infections 3. Skin abscesses or cellulitis 4. Hematogenous spread
59
The predominant cause of these skin infections. [nocardia]
N. brasiliensis
60
It has been considered the most common isolated human pathogen. [nocardia]
N. asteroides
61
It is currently believed that this Nocardia sensu stricto (type species) is rarely pathogenic. [nocardia]
N. asteroides
62
Exudative dermatitis with scab formation (dermatophilosis).
Dermatophilus congolensis
63
Actinomycetoma and other skin infections.
Nocardiopsis dassonvillei
64
Streptomyces that is an established human pathogen associated with actinomycotic mycetoma in many countries. [streptomyces]
Streptomyces somaliensis
65
They grow with mycelial forms that fragment into rod-shaped or coccoid elements—hence the term nocardioform. [spp.]
Gordonia
66
On SBA, the colonies resemble Klebsiella and can form a salmon pink pigment on prolonged incubation, especially at room temperature.
Rhodococcus
67
Agent of Whipple’s disease.
Tropheryma whipplei
68
Facultative intracellular pathogen.
Tropheryma whipplei
69
If untreated, this is a uniformly fatal disease, with typical symptoms of diarrhea, weight loss, malabsorption, arthralgia, and abdominal pain.
Tropheryma whipplei
70
Neurologic and sensory changes often occur.
Tropheryma whipplei
71
Diagnosis is best made by microscopic examination of endoscopic biopsy specimens.
Tropheryma whipplei
72
Can also detect the presence of actinomycetes. [stain]
Gomori's methenamine-silver (GMS) stain