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PH 162 > Lab - Vit B6 Def > Flashcards

Lab - Vit B6 Def Flashcards

1
Q

Xanthurenc acid is an abnormal metabolite of what amino acid?

A

Tryptophan

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2
Q

The conc of xanthurenic acid in urine is determined by procedure involving formation of ___

A

Green ferric salt

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3
Q

Final product of tryptophan load test is formed in _ medium

A

Alkaline

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4
Q

Spectrophotometer value for procedure

A

610 nm

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5
Q

In unknown tube, there is dH2O, urine and buffer + ___

A

1.7% ferric ammonium sulfate

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6
Q

Measured in urine in the expt

A

Xanthurenic acid

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7
Q

coenzyme formed fr B6, that participates in over 60 diff enzyme systems

A

pyridoxal phosphate

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8
Q

two drugs that may have antagonistic effect with B6: enumerate

A

isoniazid

oral contraceptive agents

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9
Q

measurement of xanthurenic acid follows a loading with __

A

tryptophan

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10
Q

Enumerate 3 other tryptophan intermediate metabolites, aside from xanthurenic acid

A

3-hydroxykynurenine
kynurenine
kynurenic acid

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11
Q

normal metab process: tryptophan ends up as __

A

nicotinic acid ribonucleotide

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12
Q

enzyme responsible for turning kynurenine into

kynurenic acid

A

kynurenine aminotransferase

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13
Q

enzyme responsible for turning kynurenine into

3-hydroxyurenine

A

hydroxylase

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14
Q

enzyme responsible for turning

3-hydroxyurenine into xanthurenic acid

A

kynurenine aminotransferase

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15
Q

in tryptophan load test, enumerate 2 substances that can be used as preservatives of urine

A

toluene

oxalic acid

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16
Q

after collecting 24h urine sample, what is given via oral admin?

A

L-tryptophan OR DL-tryptophan

17
Q

__ mg for 6 hour urine collection
__mg for 24 hour urine collection
> __ mg/day net increase

Fill in the blanks: abnormal xanthurenic acid excretion

A

25
100-500
25

18
Q

name 3 other biochem techniques that can be used to assess Vit B6

A

urine excretion of free VitB6
urine excretion of 4-pyridoxic acid
erythrocyte transaminase activities

19
Q

If B6 deficiency is indicated as result of tryptophan load test, oral admin of ____ daily for 7 days should reduce xanthurenic acid excretion to almost normal amounts

A

15 mg pyridoxine

PH 162 (25 decks)

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