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PH 162 > Lab - Biological Evaluation of Foods > Flashcards

Lab - Biological Evaluation of Foods Flashcards

1
Q

protein type that contains all the essential AAs in amounts that meet what is required by humans to prevent deficiency

A

complete protein

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2
Q

all animal proteins are complete proteins except __

A

gelatin

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3
Q

protein type that is too low in one or more of the essential AAs to support human growth and maintenance

A

incomplete protein

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4
Q

most plant proteins are incomplete proteins except __

A

soy

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5
Q

Combinations of incomplete proteins that, when

added together, result in a complete protein

A

complement proteins

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6
Q

Legumes and grains: incomplete protein. But they complement each other

A

legumes: lysine
grains: methionine

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7
Q

To optimize meal-stimulated protein synthesis in
muscle, there must be full complement of essential AAs
+ relatively high content of this AA

A

leucine

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8
Q

full complement of essential AAs

+ relatively high content of this AA = maximally stimulate what pathway?

A

mammalian

target of rapamycin pathway

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9
Q

Enumerate two aspects of evaluating protein quality

A

Amino acid profile (compared to “ideal” pattern)

Digestibility of the protein

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10
Q

• normally the reference protein is __because it

contains all the essential AAs

A

egg

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11
Q

Enumerate 3 factors that can limit digestibility of plant protein

A

cell wall barrier (wall can’t be digested)
lipase
trypsin

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12
Q

current “gold” standard by FAO/WHO for assessing

protein quality; incorporates digestibility as a factor in quality of proteins

A

Protein Digestibility Corrected Amino Acid Score

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13
Q

Complete proteins can often have PDCAAS values of

__

A

≥ 1.00

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14
Q

Which is false?
A.For PDCAAS, values above 1.0 are truncated.
B.PDCAAS accounts for relative AA differences among
proteins with PDCAAS for 1.0.
C.PDCAAS does not take into consideration protein ileal digestibility.

A

B

does not account dapat

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15
Q

false about Protein Efficiency Ratio
A.Assesses weight gain of growing animals on a
particular protein source (eg rats, chicks)
B.Suitable as an index for human consumption
C.Weight gain is a function of level of protein and
kind of protein

A

B

not suitable dapat

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16
Q

what percent of protein level – maximum growth? for PER

A

10%

17
Q

3 extraneous factors that may affect PER. Enumerate.

A

Season
Sensitivity of animals to care
Changes in temperature

18
Q

2 disadvantages of PER. Enumerate.

A

Weight gain is not constant with protein level

No room for allowance for maintenenance

19
Q

Which is false?
A.Nitrogen balance gives specific information on flux of protein/AA pool within tissues/organs.
B.Carbohydrate and fat are protein-sparing.

A

A

does not give dapat

20
Q

What is biologic value of protein quality?

A

Measures how much nitrogen is retained in the body

21
Q

Biologic value of protein quality is not meaningful for individual components of mixed diets. Why?

A

since limiting AAs can

differ between sources

22
Q

collective term for urinary and fecal nitrogen

losses from the protein diet

A

metabolic nitrogen

23
Q

collective term for nitrogen losses from the

protein-free diet

A

endogenous nitrogen

24
Q

negative nitrogen balance indicates ___

A

protein loss

25
Q

___ content of a meal determines its capacity to

maximally stuimulate muscle protein synthesis

A

Leucine

26
Q

Which has higher leucine?
A. whey
B. wheat
C. equal

A

A

27
Q

Which is false?
A.Turnover = lifespan; If unused, becomes excreted
B.Casein may increase muscle protein breakdown
C.Soy has lower leucine content

A

B

reduce dapat

28
Q

Lactobacillus casei, Lactobacillus rhamnosus - used for microbio assay of what substance?

A

folic acid

29
Q 
Lactobacillus fermenti , Lactobacillus plantarum - used for the ff except:
A.thiamine
B.riboflavin 
C.cobalamin
D.pyridoxine
A

C

niacin dapat

30
Q

[Microbio Assay]
Bacteria and yeast populations are treated with test
sample and quantified by __(method)

A

turbidimetry

31
Q

[Microbio Assay]

Turbidimetry: __

A

absorbance @ 540-600 nm

32
Q

scientific name of white rat

A

Rattus ratus

33
Q

2 most common rat strains used

A

Sprague Dawley

Wistar

34
Q

false about rats
A.estrus cycle: 4-5 days
B.gestation + weanling rats age: 21-23 days
C.females: 1 month old

A

C

2-3 mos dapat

PH 162 (25 decks)

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