Lab Techniques

Question 1

in a culture, make sure you have the right _____, the ______ source, and the time.

Answer 1

media

specimen

Question 2

positive gram stain retais the crystal _____

Answer 2

violet

Question 3

gram negative retain the second dye, ____

Answer 3

red

Question 4

mycobacterium have a waxy cell wall, so gram stain don’t work well. Use ___-____ stain

Answer 4

acid-fast

Question 5

india ink stain is useful for

Answer 5

encapsulated microbes

Question 6

tuberculosis is an _____-____ stain

Answer 6

acid-fast

Question 7

skin and vaginal mucosa are common specimen sources for ____________

Answer 7

wet mounts and KOH preps (KOH dissolves the epithelial cells so that the target is easily identifiable)

Question 8

if the target antibody or antigen is present in the specimen, it will produce _________

Answer 8

agglutination

Question 9

what measures complement-consuming antibody in serum or CSF?

Answer 9

complement fixation

Question 10

antibodies linked to enzymes are used to detect _____

Answer 10

antigens (ELISA)

Question 11

a western blot detects antimicrobial _______ by their reaction with target antigens

Answer 11

antibodies

Question 12

what measures an antigen or antibody in body fluids by the degree of visible precipitation of antigen-antibody complexes?

Answer 12

precipitation

Question 13

_______ shoes how sensitive the culture is to antibiotic x

Answer 13

zone of inhibition

Question 14

semi quantitative method to determine the minimal concentration of a drug that inhibits growth of a particular organism?

Answer 14

MIC. the lower the number the better

Question 15

________ are involved in acute bacterial infections and trauma

Answer 15

neutrophils

Question 16

________ is when there is a predominance of cells with _____ nuclear lobes. Liver disease, iron deficiency anemia, glucocorticoid use

Answer 16

“shift to the right”

4

Question 17

eosinophils are _______% and basophils are .5 - 1%

Answer 17

1-4%

Question 18

if absolute count is less than _________, pt may be immunocompromised

Answer 18

1000

Question 19

________ ______ _____ is characterized by an irregular monocyte nucleus that may stretch the length of the cell

Answer 19

enlarged dysmorphic lymphocyte