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Med Lab > lab safety & math > Flashcards

lab safety & math Flashcards

1
Q

glassware that has critical measurement

A
  • volumetric flask
  • volumetric pipette
  • serological pipette
  • MoHR pipette
  • micropipette
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2
Q

level III (3) water -
made how?
used for?
used as?

A
  • made thru activated charcoal filter (brita)
  • used for washing/rinsing glassware
  • used as base for making level I and II water
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3
Q

purified, practical or commercial grade used where

A

in manufacturing

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4
Q

what is LSCCLA responsible for? (3)

A
  • billing and collection of specimens appropriate to both hospitals and private labs
  • regulate who can order lab tests
  • provides license that allows hospitals or private lab to operate
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5
Q

types of reusable glass in lab

A
  • borosilicate

- quartz (clear)

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6
Q

Level I (1) water
purity?
made how?
iportant info?

A
  • purest
  • made by deionizing AND distillation
  • must be made as needed - do NOT store
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7
Q

how does a hot air oven sterilize

A

160 degrees Celsius for 1 hour continuously

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8
Q

UN 2900

A

infectious substance affecting animals only

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9
Q

united stated pharmacopeia/national formulary grade used where

A

in pharmacies

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10
Q

UN 3373

A

biological substance (category B)

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11
Q

PHIPA

A

Personal Health Information Protection Act

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12
Q

LSCCLA

A

Laboratory and Specimen Collection Centre Licensing Act

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13
Q

Class I (1) bio safety cabinet

  • appearance
  • exhaust where
  • hepa filter
  • where used
A
  • open appearance
  • vents into room or externally
  • 1 hepa fitler
  • used in pharmacy
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14
Q

KHDBDCM

A 
k-kilo
h-hecto
d-(da)deca
b-(base)gram/litre/meter
d-(d)deci
c-centi
m-milli
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15
Q

how is the MEDIAN calculated

A

central or middle value in numerical order

  • odd numbers-middle is median
  • even numbers-middle 2 numbers added together then divide by 2 is median
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16
Q

LPTP

A

laboratory proficiency testing program

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17
Q 
class II (2) biosafety cabinet
-appearance
-exhaust where
-hepa filter
used where
A
  • partial opening with 8-10” glass sash
  • vents into room or externally
  • 2 hepa filters
  • used in routine labs
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18
Q

sanitization examples (5)

A
  • 70% isopropyl alcohol
  • mild detergent
  • soap
  • alcohol based hand rubs
  • heavy organic debris present - sanitize then disinfect
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19
Q

transfer pipettes - critical? graduated?

A
  • non critical

- may be graduated

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20
Q

sanitization - surface type

A

both animate and inanimate

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21
Q

sterilization purpose

A

destroy all microorganisms and their spores

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22
Q

sterilization - surface type

A

inanimate objects

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23
Q

serological pipettes - critical? graduated?

A
  • critical

- graduated yes (measurement ends at tip)

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24
Q

bottles - critical? graduated?

A
  • non critical

- may be graduated

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25
Q

pasteure pipettes - critical? graduated?

A
  • non critical

- may be graduated

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26
Q

describe a beaker in terms of appearance and use

A
  • wide mouthed
  • straight sided
  • pour spout
  • markings to indicate volume
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27
Q

use for a beaker

A

mixing bowls for labs

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28
Q

class III (3) biosafety cabinet

  • appearance?
  • exhaust where?
  • hepa filter?
  • used when?
A
  • closed with glove box
  • vents externally ONLY
  • minimum 2 hepa filters - usually 3
  • used for high risk (anthrax, TB, ebola
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29
Q

microbiology sub-departments

A
  • bacteriology
  • virology
  • parasitology
  • mycology
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30
Q

where do graduations end on MoHR pipette

A

above the tip

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31
Q

reagent grade/analytical reagent grade - level of purity

A

highest degree of purity

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32
Q

UN 2814

A

infectious substance affecting humans

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33
Q

blow out

A

squeeze out last drop

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34
Q

where does category B box go

A

ALWAYS public health laboratory

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35
Q

UN 1845

A

dry ice/carbon dioxide inside

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36
Q

what glassware is used to measure 24-hour urine

A

graduated cylinder

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37
Q

type of disposable glass in lab

A

flint

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38
Q

technical grade - purity level

A

lowest purity

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39
Q

what does an ORANGE band indicate on a pipette

A

10ml

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40
Q

test tube - critical? graduated?

A
  • non critical

- may be graduated

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41
Q

beakers - critical? graduated?

A
  • non critical

- may be graduated

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42
Q

graduated cylinder - critical? graduated?

A
  • non critical

- ALWAYS graduated

43
Q

what does PHIPA govern

A
  • regulate the sharing of information between HC providers and other organizations
  • sets standards for confidentiality in HC
44
Q

UN 3291

A

clinical waste/medical waste

45
Q

what is neutral pH

A

neutral pH is 7

46
Q

pH above neutral

A

alkaline above 7

47
Q

pH below neutral

A

acidic below 7

48
Q

transfusion services sub-category

A

maternal/fetal compatibility

49
Q

reusable plastic used in lab

A

polypropolyne

50
Q

what is a micropipette used for

A

measure microliters

51
Q

what does pH meter measure?

to determine what?

A
  • measures potential hydrogen

- determine alkalinity or acidity

52
Q

when sterilizing metal, auto clave must operate how

A

121 degrees Celcius for 15 minutes at 15psi

53
Q

disinfection - surface type

A

inanimate

54
Q

MoHR pipettes - critical? graduated?

A
  • critical

- not graduated (measurements end above the tip)

55
Q

Florence flask - critical? graduated?

A
  • non critical

- NEVER graduated

56
Q

volumetric flask - critical? graduated?

A
  • critical

- NEVER graduated

57
Q

Erlenmeyer flask - critical? graduated?

A
  • non critical

- may be graduated

58
Q

pH meter use

A

measure potential of hydrogen to determine alkalinity or acidity

59
Q

who does PHIPA include (5)

A
  • labs
  • doctors/hospitals
  • employers
  • schools
  • insurance companies
60
Q

how is pH meter calibrated

A

kept moist in buffered solution of distilled water to maintain neutral pH

61
Q

most common hematology test?

color tube?

A
  • INR (international normalized ratio)

- light blue tube

62
Q

to contain

A

rinse in final solution

63
Q

how is the MEAN calculated

A

average of all values added together, then divided by number of values

64
Q

how is SOLUTE CALCULATED

A

solute over solution

% over 100 = x over solution

65
Q

how is REAGENT equation calculated

A

part 1
reagent a over reagent a+b = x over total volume
part 2
solute + solvent (x) = solution

66
Q

what is LPTP responsible for

A

sends blind samples to determine proficiency in testing specimens

67
Q

disposable plastic used in lab

A

polyethylene

68
Q

level II (2) water
purity
made how
used where

A
  • mid purity
  • made by either deionizing or distillation
  • used in most routine lab procedures
69
Q

what are we looking for when calculating the mode

A

precision

70
Q

what are pH meter electrodes called

A
  • reference/calomel probe

- glass probe

71
Q

3 controls required with each use of autoclave

A
  • temperature control
  • biohazard strip
  • chemical control
72
Q

departments that blood specimens are sent to (5)

A
  • hematology
  • clinical chemistry
  • microbiology
  • histopathology
  • transfusion services
73
Q

reagent grade/analytical reagent grade used for

A
  • research
  • producing controls
  • standards and buffers
74
Q

disinfection purpose

A

reduce levels of microorganisms to a safe handling level

75
Q

where do graduation end on serological pipette

A

end at the tip

76
Q

united stated pharmacopeia/national formulary grade purity level

A

less pure than chemical pure

77
Q

what does a BLUE band indicate on a pipette

A

5ml

78
Q

vortex mixer use

A

tornado action to mix fluids in tubes (not good for delicate body fluids ex blood)

79
Q

volumetric pipette - critical? graduated?

A
  • critical

- never graduated

80
Q

sanitization purpose

A

reduce levels of microorganisms to a safe handling level

81
Q

micropipette - critical? graduated?

A
  • critical

- never graduated

82
Q

what does RED band indicate on a pipette

A

1ml

83
Q

technical grade used where

A

industries

84
Q

what is the goal of autoclave

A

kill all microorganisms and their spores

85
Q

autoclave use

A

sterilizing metal equipment

86
Q

hot air oven uses (3)

A
  • incubate cultures
  • dry glassware and metal equipment
  • catalyst (enhances) and desiccants (absorbs moisture)
87
Q

what does RHPA do (6)

A
  • regulates professionals registered with college
  • protect public
  • professional accountability
  • prevents working outside scope
  • students are delegated
  • graduate deemed competent
88
Q

what 2 things does the OHSA do

A
  • sets out rights and requirements for employer and employee

- specifies workers right to health and safety

89
Q

hematology sub-department

A

coagulation studies

90
Q

how is the MODE calculated

A

most frequent occurring number (can have more than 1)

91
Q

clinical chemistry sub-department

A

immunology

92
Q

reagent grades - order of purity

A
  1. reagent grade/analytical reagent grade
  2. chemical pure
  3. united stated pharmacopeia/national formulary grade
  4. purified, practical or commercial grade
  5. technical grade
93
Q

chemical pure used for

A

laboratory purposes

94
Q

histopathology sub-departments

A
  • cytology

- cryogenics

95
Q

uses for hot air oven

A
  • incubate cultures
  • dry glassware
  • catalysts and desiccants
  • heat fix tissue to slide after warm bath
96
Q

disinfection examples (6)

A
  • 10% bleach
  • phenol products (lysol)
  • 70% iospropyl alcohol
  • 2% glutaraldehyde
  • Virkon
  • ammonia
97
Q

antisepsis surface type

A

animate objects

98
Q

purified, practical or commercial grade purity level

A

low purity rate

99
Q

to deliver

A

retain last drop in pipette

100
Q

incubator use

A

growing microorganism for 24 hour incubation period

101
Q

antisepsis examples (7)

A
  • alcohol based hand rub
  • 70% isopropyl alcohol
  • 3% hydrogen peroxide
  • chlorhexidine (not on babies)
  • tincture of iodine
  • paridone iodine
  • soap and water
102
Q

antisepsis purpose

A

reduce levels of microorganisms to safe handling level to avoid spread of contagions

103
Q

sterilization examples (5)

A
  • autoclave
  • hot air oven
  • incineration
  • radiation
  • toxic gases
104
Q

bacti-cinerator uses (2)

A
  • heat fix smears

- sterilize inoculation loops

Med Lab (13 decks)

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