culture media

Question 1

additives - water purposes

Answer 1

• transport nutrients and waste

- dissolves (solvent) ingredients

Question 2

additives - water example

Answer 2

distilled

Question 3

additives - peptones and carbohydrates purpose

Answer 3

source of energy and nutrients

Question 4

additives - peptones and carbohydrates examples

Answer 4

• beef heart infusion
• tryptic soy
• lactose
• glucose

Question 5

additives - blood products purpose

Answer 5

nutrients

Question 6

additives - blood products examples

Answer 6

• whole blood
• lysed or heated blood
• sheep and horse blood
• serum

Question 7

additives - selective agents purpose

Answer 7

inhibits specific bacteria

Question 8

additives - selective agents examples

Answer 8

• bile salts
• dyes
• alcohols
• antibiotics

Question 9

additives - indicators purpose

Answer 9

detect changes in pH, oxygen, growth of bacteria

Question 10

additives - indicators examples

Answer 10

• bromothymol blue
• neutral red
• acid fuchsin for pH
• resazurin for oxygen

Question 11

additives - agar purpose

Answer 11

gives consistency to media (no nutritional value)

Question 12

additives- agar example

Answer 12

0.05-5%

Question 13

additives - additives purpose

Answer 13

• maintain pH
• absorb toxins
• supply extra growth needs

Question 14

additives - additives examples

Answer 14

• buffers for pH
• charcoal and starch to absorb toxins
• vitamins for growth

Question 15

agar temp - 90-100 degrees celcius

Answer 15

dissolves

Question 16

agar temp - 38-42 degrees celcius

Answer 16

solidifies

Question 17

agar temp - 45-50 degrees celcius

Answer 17

molten (add heat sensitive ingredients)

Question 18

agar temp - 60 degrees celcius

Answer 18

add blood to denature

Question 19

agar temp - 50 degrees celcius

Answer 19

to make whole blood agar

Question 20

agar temp - 45-50 degrees celcius

Answer 20

laked blood (skirrow’s_

Question 21

agar temp - 85 degrees celcius

Answer 21

• serum added to solidify = inspissation (Loeffler’s)

- serum also added to enrich (extra nutrients)

Question 22

agar concentration - 0%

Answer 22

fluid bath

Question 23

agar concentration - 0.05-0.1%

Answer 23

reduce convection current

Question 24

agar concentration - 0.5%

Answer 24

semi-solid (slushy)

Question 25

agar concentration - 2%

Answer 25

solid

Question 26

agar concentration - 3-5%

Answer 26

prevent swarming

Question 27

basic culture media

Answer 27

• solid or broth
• simple
• inexpensive
• nutrients for non fastidious

Question 28

enriched culture media

Answer 28

• solid or broth
• substances added to basic media
• nutrients for fastidious

Question 29

enrichment culture media

Answer 29

• broth

- inhibit normal flora, allow pathogen to grow

Question 30

selective culture media

Answer 30

• solid or broth

- inhibit one, allow antoher to grow

Question 31

differential culture media

Answer 31

• solid

- shows colony growth

Question 32

selective/differential sulture media

Answer 32

• allows pathogen growth

- has indicators to tell them apart

Question 33

whole blood uses

Answer 33

all routine specimens but feces

Question 34

whole blood enriched

Answer 34

blood

Question 35

whole blood differential

Answer 35

hemolysis

Question 36

whole blood selective

Answer 36

no

Question 37

whole blood enrichment

Answer 37

no - solid

Question 38

whole blood incubation

Answer 38

o2, co2, anaerobic

Question 39

chocolate uses

Answer 39

• genital, eye, ear, respiratory

Question 40

chocolate enriched

Answer 40

lysed

Question 41

chocolate differential

Answer 41

no

Question 42

chocolate selective

Answer 42

no

Question 43

chocolate enrichment

Answer 43

no - solid

Question 44

chocolate incubation

Answer 44

co2