Lab Quiz 4: Viable Bacteria Counts and Bacteriophage Counts (Bio 286 - Microbiology) Flashcards
1
Q
countable plates
A
plates with 30-300 colonies
2
Q
CFU
A
number of “colony forming units”
3
Q
TDF
A
tube dilution factor
4
Q
TDF calculation
A
(sample + diluent)/sample
5
Q
SDF
A
series dilution factor
6
Q
SDF calculation
A
TDF 1 x TDF 2 x TDF 3…
7
Q
CFU/mL
A
(CFU x SDF)/volume plated (in mL)
8
Q
Tube Dilution Factor (TDF)
A
(volume added + diluent volume)/volume added
9
Q
series dilution factor (SDF)
A
product of all tube dilution factors up to and including that tube; TDF a x TDF b x TDF c…
10
Q
final dilution factor (FDF)
A
SDF / volume plated
11
Q
enumerate
A
estimate the number of
12
Q
T4
A
lytic phage of E. coli; sometimes referred to as a coliphage
13
Q
plaque
A
holes in the bacterial lawn resulting from viral propagation overwhelming the host cell growth rates
14
Q
PFU/mL
A
(PFU x SDF)/volume plated (in mL)
15
Q
PFU
A
number of “plaque forming units”
