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BIO 286 - Microbiology > Lab Quiz 1: Microscopy, Cell morphology, Aseptic Technique (Bio 286 - Microbiology) > Flashcards

Lab Quiz 1: Microscopy, Cell morphology, Aseptic Technique (Bio 286 - Microbiology) Flashcards

1
Q

ocular lenses (binocular)

A

eye pieces that each has a magnifying power of 10X

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2
Q

cord wrap

A

brackets to store the power cord around when done using the scope

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3
Q

objective lenses

A

secondary lenses to magnify the sample ranging in magnifying power from 4X to 100X

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4
Q

course focus knob

A

used to rapidly move the stage (and thus the sample) to focus

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5
Q

fine focus knob

A

used to tweak the focus with 40X and 100X objectives

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6
Q

mechanical stage controls

A

move the sample in the X and Y planes, where the focus is the Z plane

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7
Q

condenser iris

A

the lever that controls how much light passes through the sample

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8
Q

power switch

A

turns the lamp on and off

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9
Q

light source

A

halogen lamp providing light

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10
Q

parfocal and parcentered

A

once the sample is centered and focused with one objective, it will be (more or less) focused and centered for all other objectives too

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11
Q

total magnification

A

ocular lens (10X) x objective lens

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12
Q

field of view

A

total area of the sample that is visual; decreases as magnification increases; equal to (division size x 100)/0.56 (because the entire ocular reticle only spans 56%)

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13
Q

working distance

A

space between objective lens and sample; decreases as magnification increases; measured with a ruler

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14
Q

right ocular lens

A

has the measuring reticle (scale) with 100 unit-less divisions; calibrated with a slide with a stage micrometer (where the slides have a line etched on them that is 1mm, 1000 micrometers long and divided into 100 equal segments that are 10 micrometers each)

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15
Q

division size calculation

A

(number of scale divisions x 10 micrometers) / number of ocular divisions

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16
Q

coccus

A

spherical bacterium

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17
Q

diplococci

A

spherical bacteria growing in pairs

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18
Q

diplococci encapsulated (pneumococcus)

A

spherical bacteria growing in pairs within an encapsulated membrane

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19
Q

staphylococci

A

spherical bacteria growing in clusters

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20
Q

streptococci

A

spherical bacteria growing in chains

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21
Q

sarcina

A

cubical packet of 8, 16, or more spherical bacteria

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22
Q

tetrad

A

square packet of four spherical bacteria

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23
Q

enlarged rod (fusobacterium)

A

bacteria rod that is elongated into a slender, pointed end shape

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24
Q

vibrio

A

comma shaped bacteria

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25
Q

comma’s form (bdellovibrio)

A

a monotrichous (one flagella) comma shaped bacteria

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26
Q

club rod (corynebacteriaceae)

A

a comma shaped bacteria with most of the cell material condensed at one end and the other end coming to a narrow handle, similar to a club

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27
Q

helical form (helicobacter pylori)

A

a comma shaped bacteria that has an two ends meeting at a more narrow midpoint, resembling a helical shape

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28
Q

coccobacillus

A

short round rod bacteria

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29
Q

bacillus

A

rod shaped bacteria

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30
Q

diplobacilli

A

pairs of bacilli

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31
Q

palisades

A

bacilli in a parallel or picket fence

32
Q

streptobacilli

A

chains of bacilli

33
Q

hypha

A

the narrowest part of bacteria with a shape similar to the club rod but with a more slender head

34
Q

stalk

A

the most narrow, slender part of a comma shaped bacteria

35
Q

corkscrew’s form (borrelia burgdorferi)

A

a thick spiral bacteria

36
Q

spirochete

A

spiral shaped bacteria

37
Q

filamentous

A

complex bacteria shape that looks like an unraveled spiral

38
Q

aseptic

A

sterile, not contaminated

39
Q

axenic

A

pure culture

40
Q

morphological features describing colonies

A

size, shape, margin, elevation, color, consistency, surface, opacity

41
Q

size of colonies

A

punctiform (pinpoint), small, moderate, large

42
Q

shape of colonies

A

round, irregular, fibrous, rhizoid, curled

43
Q

round

A

colonies that have a nice radial symmetry and appear as round mounds of growth

44
Q

irregular

A

colonies that have less symmetry and have undulating edges

45
Q

fibrous shape

A

soil bacteria that have fibrous or hairy colonies

46
Q

rhizoid

A

bacteria and fungi that have a spreading and branching type of growth

47
Q

curled

A

bacteria that have a terraced appearance due to swarming on the plate

48
Q

margin of colonies

A

fibrous, entire, lobate, serrate

49
Q

fibrous margin

A

colonies that have a filamentous edge

50
Q

entire

A

colonies that with a smooth and even edge

51
Q

lobate

A

colonies that have an irregular, lobed edge

52
Q

serrate (aka erose)

A

colonies that have a saw-tooth edge

53
Q

elevation of colonies

A

flat, raised, convex, crateriform, umbonate, pitted

54
Q

flat

A

colonies that have very little elevation and remain flat on agar surface

55
Q

raised

A

colonies that have some elevation and appear somewhat raised from agar surface

56
Q

convex

A

colonies that form a substantial pile of cells with a dome-like structure

57
Q

crateriform (aka concave)

A

colonies that exhibit depressions in the center

58
Q

umbonate

A

colonies that exhibit an extra dimple in the center

59
Q

pitted

A

colonies that metabolize the agar in the media so they grow within the media

60
Q

color of colonies

A

variants of cream to tan, but some white, black, yellow, red, pink, purple, and other shades

61
Q

consistency of colonies

A

butyrous, viscous, mucous, friable

62
Q

butyrous

A

colonies having a buttery consistency

63
Q

viscous

A

colonies having a sticky consistency

64
Q

mucous

A

colonies having a slimy consistency (often due to production of a capsule)

65
Q

friable

A

colonies (like mycobacteria) that have hard and waxy colonies that break up upon probing

66
Q

surface of colonies

A

appear to be smooth and/or shiny or dull/rough or wrinkled

67
Q

opacity of colonies

A

varying translucence, but most appear opaque (not transparent)

68
Q

plate streaking

A

a lab technique used to obtain isolated individual colonies of bacterial clones

69
Q

process of plate streaking

A

sterilize a wire loop by incineration and let cool for about 10 seconds -> get loopful of mixed culture and apply it to the plate in section 1 -> sterilize loop again and cool -> use loop to spread some cells of section 1 to section 2, spread in section 2 -> sterilize loop and cool -> use loop to spread some cells of section 2 to section 3, spread in section 3 -> sterilize loop and cool -> use loop to spread some cells of section 3 to section 4, spread in section 4 -> sterilize loop, place plate in incubator

70
Q

Roccal

A

antiseptic used to wipe the workspace down (before and after lab sessions)

71
Q

regular trash disposal

A

into regular trash bins

72
Q

stain disposal

A

labeled carboy at the front of the lab

73
Q

glass disposal

A

cardboard glass receptacle

74
Q

chemical disposal

A

containers and instruction for disposal provided on a case to case basis

75
Q

contaminated material disposal

A

orange autoclave bag at the front of lab; contaminated glassware in a bin at the front of the lab for later decontamination

76
Q

bunsen burner

A

decently sized triangle of blue flame = good

BIO 286 - Microbiology (21 decks)

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