Ch 16: Bacterial Diversity (Bio 286 - Microbiology) Flashcards
common traits of bacteria
central apparatus of gene expression: RNA polymerase, ribosomal RNAs, translation factors; cells walls made of peptidoglycan
gram positive bacteria
rods and cocci; no outer membrane; thick cell wall with peptidoglycan; many are pathogens; include staphylococcus, streptococcus, and Listeria
Listeria
polymerizes actin inside host cell
(Gram positive) Lactobacillales
key genera: Lactobacillus and Streptococcus; non-sporulating, oxidase-/catalase-negative fermentative rod bacteria that produce lactic acid; widely used in food production and preservation; obtain energy only by substrate-level phosphorylation; aerotolerant anaerobes (not sensitive to oxygen); homofermentative produce only lactic acid while heterofermentative produce ethanol, CO2, and lactate
lactobacillales
aerotolerant anaerobes
(Gram positive) lactobacillus
make yogurt, milk, bread
(gram positive) streptococcus
causes strep throat and cavities
(gram positive) leuconostoc
on cabbage and makes sauerkraut
(gram positive) nonsporulating bacillales and clostridiales
key genera: Listeria, Staphylococcus, Sarcina Listeria; in the order of Bacillales; found widely in soils; opportunistic pathogen that causes foodborne illnesses; catalase-positive rod shaped; facultatively aerobic chemoorganotrophs; often grow well at low temperatures
(gram positive) staphylococcus
facultative aerobe that respires but can ferment; grows in clusters; CATALASE-POSITIVE; resistant to reduced water potential and tolerate drying in high salt (NaCl); common commensals and parasites of humans/animals that occasionally cause serious infections
staphylococcus
tolerate drying from high salt
(gram positive) sporulating bacillales and clostridiales
key genera: Bacillus, Clostridium, Sporosarcina; distinguished on basis of cell morphology, shape, and cellular position of endospore, relationship to oxygen, and energy metabolism,; generally found in soils; pathogenic species are saprophytic and infect animals incidentally; endospores are advantageous for soil microorganisms because of variations in nutrient levels/temperature/water activity
(Gram positive) Bacillales/Clostridiales
endospore forming bacteria
gram positive endospores
extremely heat resistant; spore forms inside mother cell
(gram positive) Clostridium
toxin formers of tetanus, botox, and gangrene
(gram positive) Bacillus
toxin formers of Anthrax, Bt
(gram positive) Bacillus and Paenibacillus
many produce extracellular hydrolytic enzymes that break down polymers (polysaccharides, nucleic acids, lipids); many bacilli produce antibiotics, while others produce toxic insecticidal proteins
(gram positive) Clostridium
lacks a respiratory chain and obtains ATP by substrate-level phosphorylation; SACCHAROLYTIC ferment surgars; PROTEOLYTIC ferment amino acids or amino acid pairs (stickland reactions); mainly found in anaerobic pockets in soil or mammalian intestinal tract; some are pathogenic causing botulism, tetanus, and gangrene
saccharolytic clostridium
ferment sugars
proteolytic clostridium
ferment amino acids or amino acid pairs (stickland reactions)
clostridium
causes botulism, tetanus, and gangrene
(gram positive) tenericutes (the mycoplasmas)
key genera: Mycoplasma, Spiroplasma; lack cell walls; some of smallest organisms; related to Firmicutes (nonsporulating bacillus and clostridia); typically live in close association with animal and plant hosts; small genomes
(gram positive) firmicutes
nonsporulating bacillus and clostridium; low G+C content
(gram positive) mycoplasmas
small, pleomorphic cells; colonies show a characteristic “fried egg” appearance
(gram positive) actinobacteria
key genera: Arthrobacter, Corynebacterium, Propionibacterium; rod-shaped to filamentous, usually aerobic inhabitants of soil and plant materials; mostly harmless commensals with exception of Mycobacterium; valuable for antibiotics and certain fermented dairy products; have a higher GC composition in DNA