Lab Quiz Flashcards
Ocular lens magnifictaion and placement in microscope
10
Lens u look through
Compound microscope and what lenses?
Uses 2 sets of lenses to increase six of objects
Composed of:
-ocular lens
-objective lens
Objective lens
Near stage
Magnifying power differed between each objective lens
How do you calculate the magnifying power
10(ocular lens) x objective lens
Feild of view
Size of are that the lens views
Larger magnifying power implies
Smaller area viewed
Why is it important to center the specimen on low before switching to high
When switching from low to high you are looking at the central point of the specimen but if u were to focus it in high power the field of view is smaller therefore it is easier to focus on not the center
Par focal capability
Traitor a good microscope is when u focus the specimen under low power and switch it to high it remains in reasonably good focus
How do stains work
They bind to various macromolecules and structures in the cell which makes them more visible
Why are living cells stained
To show cilia flagella and nucleus
Vital stains
Kills cells more slowly
Organisms absorb stains and continue to carry on thier life functions for some time
Lugols solution
Composed of iodine and water
Stains carbohydrates like attaches and glycogen
Cytoplasmic streaming
Chloroplasts are moving along inside of cell wall because cytoplasm is in motion
Plane of focus
A Position a specific distance from the lenses where an object appears in sharp focus
Depth of field
Thickness of layer that is sharp in focus
