lab quiz Flashcards
1
Q
ladder
A
specifically cut DNA fragments to gauge unknown fragment sizes
2
Q
how does gel electrophoresis separate DNA fragments?
A
it uses charge to separate DNA by length
3
Q
which way does DNA go in gel electrophoresis?
A
towards + end
4
Q
gel loading buffer
A
moves independently from DNA, and when it stops at the end, you know that the DNA sample is all spread out through the gel.
5
Q
ethidiium bromide function in gel electrophoresis
A
it bonds to DNA and can be seen under UV lights (lets us actually see our DNA)
6
Q
ligation
A
to glue together
7
Q
t4 DNA ligase needs food…
A
T4 DNA ligase buffer
8
Q
what samples did we put on the gel?
A
-UD gDNA
-D gDNA
-UD vector DNA
-D vector DNA
-molecular weight ladder
