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Microbiology > Lab Exam 2 > Flashcards

Lab Exam 2 Flashcards

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Biology 101 flashcards
1
Q

The Durham tube inverted in a tube of Phenol Red Broth detects the production of which of the following?

A

gas

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2
Q

Catalase converts which of the following chemicals to oxygen and water?

A

hydrogen peroxide

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3
Q

Yellow and no bubble in the Durham tube is an indication of

A

A/-
acid

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4
Q

The color of phenol red at an acid pH is?

A

yellow

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5
Q

A growth medium that favors the growth of some microorganisms but inhibits the growth of other microorganisms is a ________________ medium.

A

selective

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6
Q

solid media

A

-2% agar
- can see colony morphology, pigmentation, hemolysis
-examples are Nutrient Agar and Trypticase Soy Agar

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7
Q

liquid media

A

-no agar
-for inoculum preparation
-Examples are nutrient broth, trypticase soy broth

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8
Q

semi-solid medium

A

.5% agar
motility medium

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9
Q

special purpose media

A

aids in the isolation and identification of specific microorganisms

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10
Q

selective media

A

inhibit the growth of one organism and permits the growth of another
-helpful when separating a mixture of gram-positive and gram-negative

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11
Q

differential media

A

cause an observable color change when a particular chemical reaction occurs

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12
Q

Phenylethyl Alcohol Agar (PEA)

A

selective media
allows the growth of gram-positive and inhibits the growth of gram-negative
used to isolate staph and strep

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13
Q

MacConkey (MAC)

A

-selective and differential
-the selective ingredients are bile salts which inhibit gram positive
-differential ingredient is lactose

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14
Q

If fermentation occurs on MAC agar, what color would the indicator be?

A

pink-red

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15
Q

Mannitol Salt Agar

A

-selective and differential
-selects for staph
-differential ingredient is sugar mannitol
-7.5% NaCl

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16
Q

If fermentation occurs on Mannitol Salt Agar, what color is the indicator?

A

yellow

17
Q

Phenol red pH indicator

A

yellow for acid and fuchsia pink for basic
differential
uses glucose, sucrose, and lactose

18
Q

Eosin Methylene Blue

A

differential and selective medium
the differential indicator dyes are eosin Y and methylene blue

19
Q

If fermentation occurs on EMB agar, what color is it?

A

large amounts of acid are green and small amounts are pink

20
Q

What two gram negative bacterias grow on EMB?

A

E coli and pseudomonas aeruginosa (no lactose)

21
Q

Citrate test

A

detect the ability of certain bacteria to utilize citrate as the sole source of carbon
-the indicator is bromothymol blue

22
Q

If citrate is used, what color does the indicator turn if alkali is used)

A

blue

23
Q

If citrate and acid is used, what color does the indicator turn?

A

yellow

24
Q

What color indicates citrate negative?

A

green

25
Q

Fermentation tubes

A

ability to ferment a specific sugar is dependent on enzymes and F tube use phenol red detect gas production

26
Q

Starch test

A

differential
iodine is the indicator

27
Q

fermentation

A

the incomplete break down of substrates

28
Q

In phenol red, what does red broth and no bubble indicate?

A

no fermentation, no acid
-/-

29
Q

In phenol red, what does yellow broth with a bubble?

A

A/G
acid and gas production

30
Q

In Simmons Citrate Agar, what does green agar indicate?

A

-
citrate is not utilized

31
Q

In Simmons Citrate Agar, what does blue agar indicate?

A

+
citrate is utilized

32
Q

In Triple Sugar Iron, what does an alkaline slant and acid butt indicate?

A

K/A
bacteria ferments glucose

33
Q

In TSI, what does an acid slant, acid butt, and gas production indicate?

A

A/A, G
bacteria ferments glucose, sucrose, lactose and making acid

34
Q

In TSI, what does an alkaline slant, acid butt, and H2S production?

A

K/A, H2S
bacteria ferments glucose and makes acid
hydrogen sulfide is also formed

Microbiology (15 decks)

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