Lab: Digestive Chemicals Flashcards
Substance on which a catalyst works
Substrate
Biologic catalyst protein in nature
Enzyme
Increases the rate of a chemical reaction without becoming part of the product
Catalyst
Provides a standard of composition for test results
Control
List three characteristics of enzymes
- substrate specific
- temperature specific
- pH specific
The enzymes of the digestive system are classified as hydrolases. What does this mean
Hydrolases breaks down organic food molecules by adding water to the molecular bonds to cleave them
Salivary amylase is produced by what organ
Salivary gland
Site of action for salivary amylase
Oral cavity or mouth
Substrate of salivary amylase is
Starch
Optimal pH of salivary amylase is
6.7-7.0
Trypsin is produced by what organ
Pancreas
Site of action for trypsin is
Small intestine
Substrate of trypsin
Proteins
Optimal pH of trypsin
8.0
Lipase is produced by what organ
Pancreas
Site of action for lipase is
Small intestine
Substrates of lipase are
Fats and proteins
Optimal pH of lipase is
7.4-8.0
The end products of proteins are
Amino acids
The end products of fats are
Fatty acids and glycerol
The end products of carbohydrates are
Simple sugars glucose
How does the substrate for amylase differ from the substrate for lactase
Substrate of amylase is starch polysaccharides and substrate of lactase is disaccharides
How is the substrate for amylase similar to the substrate for lactase
Site of action is small intestine for both carbohydrates
Where does lactose hydrolysis occur for lactase-persistent individuals
Small intestines
Where does lactose hydrolysis occur for lactose-intolerant individuals who have consumed probiotic bacterial microflora
Large intestine
BAPNA
Used to test for protein hydrolysis which was indicated by a yellow color
Lugol’s iodine
Used to test for the presence of starch which was indicated by blue-black color
Litmus
Used to test for the presence of fatty acids which was evidenced by a color change from blue to pink
Benedict’s solution
Used to test for the presence of reducing sugars maltose, sucrose, glucose as indicated by a blue to green or organge color change
What conclusions can you draw when an experimental sample gives both a positive starch test and a positive maltose test after incubation
Starch digestion is partial into maltose
Why was 37 degrees C the optimal incubation temperature
It is body temperature
Trypsin is a protease similar to pepsin the protein digesting enzyme in the stomach. Would trypsin work well in the stomach why
No because the pH for trypsin is slightly basic the pH optimum for pepsin is acidic
What role does bile play in fat digestion
Emulsification of fat by bile increases the surface area for lipase activity
Assume you have been chewing a piece of bread for 5 to 6 minutes how would you expect it’s taste to change during this interval why
The bread would begin to taste sweet because starch is broken down to glucose by amylase
Mechanism of absorption for monosaccharides
Passive transport
Mechanism of absorption for fatty acids and monoglycerides
Active transport
Mechanism of absorption for amino acids
Active transport