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Bacteriology > Lab 8 - Pasteurella, Mannheimia, Bibersteinia, Ornithobacterium, Riemerella, Actinobacillus, Haemophilus, Histophilus, Avibacterium, Taylorella. Identification of an unknown bacterium I. > Flashcards

Lab 8 - Pasteurella, Mannheimia, Bibersteinia, Ornithobacterium, Riemerella, Actinobacillus, Haemophilus, Histophilus, Avibacterium, Taylorella. Identification of an unknown bacterium I. Flashcards

1
Q

P. multocida can cause haemolysis on blood agar

A

False

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2
Q

Pneumonia in laboratory rodents is caused by P. caballi.

A

False, (by P. Pneumotropica)

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3
Q

Actinobacillus equuli can cause pyosepticaemia in horses

A

True

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4
Q

Mannheima haemolytica can grow on MacConkey agar.

A

True

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5
Q

Athrophic rhinitis of swine is caused by P. multocida B.

A

False, (by P. multocida D)

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6
Q

P. multocida has five capsule types

A

True

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7
Q

Pasteurella spp. is catalase-negative and oxidase-positive bacteria.

A

False, (both catalase-positive and oxidase-positive bacteria)

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8
Q

Members of the family of Pasteurellaceae are able to live in the environment for a long time.

A

False, (only sort time due to dehydration and cannot replicate in the environment either)

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9
Q

Members of family of Pasteurellaceae can be found on mucous membranes (respiratory tract).

A

True

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10
Q

Histophilus somni needs carbon-dioxide (CO2) for culturing.

A

True

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11
Q

Glässer-disease of piglets is caused by Haemophilus parasuis.

A

True

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12
Q

Swine haemorrhagic-necrotic pleuropneumonia is caused by Actinobacillus suis.

A

False, (by A. pleuropneumoniae)

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13
Q

Taylorella equigenitalis is the causative agent of contagious equine metritis (CEM).

A

True

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14
Q

Cattle lumpy jaw is caused by Actinobacillus lignieresii.

A

False, (by Actinomyces bovis)

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15
Q

Acute systemic pasteurellosis of feedlot lambs is caused by P. multocida D.

A

False, (by B. trehalosi)

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16
Q

Mannheimia haemolytica can cause pneumonia (shipping fever) in cattles.

A

True

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17
Q

Avibacterium paragallinarum needs CO2 and V-factor during culturing.

A

True

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18
Q

Biotype 1 strains of Actinobacillus pleuropneumoniae need X-factor for growth.

A

False, (V-factor demand)

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19
Q

P. multocida can cause fowl typhus.

A

False, (fowl cholera)

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20
Q

On the basis of cell wall antigens P. multocida have 11 serotypes in Heddleston-system.

A

False, (Heddleston system:16, Namioka system: 11)

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21
Q

P. multocida can grow on MacConkey agar.

A

False, (Pasteurella species will not grow on MacConkey agar)

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22
Q

Pasteurella species are non-fastidious bacteria; nutrient agar is enough for isolation.

A

False, (fastidious and cannot culture on nutrient agar or nutrient broth)

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23
Q

R. anatipestifer need V-factor for propagation

A

False

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24
Q

O. rhinotracheale can be found on the mucous membranes of respiratory tract of turkey.

A

True, (also chicken)

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25
Q

H. somni need V-factor for growth.

A

False, (X, V factor not needed)

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26
Q

Glässer-disease is caused by A. suis.

A

False, (by H. parasuis)

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27
Q

A. pleuropneumoniae biotype 1 need X-factor and CO2 for propagation.

A

False, (V-factor and do not need CO2)

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28
Q

P. multocida can cause septicaemia in rabbit.

A

True, P. multocida A, D.

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29
Q

M. haemolytica have 4 capsule types

A

False, (12 types)

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30
Q

P. multocida capsule type B have a hyaluronic acid capsule.

A

False

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31
Q

M. haemolytica have a characteristic smell, like chestnut.

A

False

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32
Q

Pigeon is the most susceptible species to Riemerella anatipestifer infection.

A

False, (water fowl)

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33
Q

Actinobacillus suis can cause beta-haemolysis on blood agar.

A

True

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34
Q

B. trehalosi can cause septicaemia in lambs

A

True, 4-10 month old lambs

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35
Q

B. treholasi have 4 capsule types.

A

True

36
Q

Pasteurella is not fastidious?

A

False

37
Q

Taylorella (H.) equigenitalis needs V-factor?

A

False, (X and V factor not needed)

38
Q

A. seminis can cause epididymitis?

A

True

39
Q

Avibacterium paragallinarum needs CO2 for culturing.

A

True, require an enriched CO2(5–10%) atmosphere

40
Q

Glässer-disease of piglets is caused by A.pleuropneumoniae.

A

False, (by H. parasuis)

41
Q

Taylorella equigenitalis can be found on the mucous membrane of genitals of stallions only.

A

False, (also clitoris of mares)

42
Q

M. haemolytica can cause arthritis in cattle’s.

A

True

43
Q

P. multocida have a characteristic smell, like chestnut.

A

True

44
Q

NAD is need for the propagation of T. equigenitalis.

A

False

45
Q

O. rhinotracheale is a capnophilic bacterium.

A

False, (only 10% CO2)

46
Q

A. paragallinarium need X-factor and CO2 for propagation.

A

False, (CO2 and V-factor demand)

47
Q

Glässer-disease is caused by H. somni

A

False, (by H. parasuis)

48
Q

Genera of the Family Pasteurellaceae

A
  • Pasteurella, Mannheimia, Bibersteinia
  • Actinobacillus
  • Haemophilus, Histophilus, Avibacterium
  • Other genera relating having shared characteristics with the Family Pasteurellaceae
  • Ornithobacterium
  • Riemerella
  • Taylorella
49
Q

PASTEURELLACEAE

  1. Habitat
  2. Morphology
  3. Staining
  4. Culture
A
  1. Habitat: mucous membranes (respiratory way, genitals, alimentary tract)
  2. Morphology: 0.5-2 µm coccoid rods
  3. Staining: Gram-negative, bipolar staining
  4. Culture:
    • fastidious (yeast extract, blood, pH-susceptibility)
    • aerobic – facultative anaerobic
50
Q

PASTEURELLA

  1. Habitat
  2. Morphology
  3. Staining
A
  1. Habitat: mucous membranes (respiratory tract)
  2. Morphology: 0.5-2 µm coccoid rods
  3. Staining: Gram negative, bipolar staining
51
Q

MANNHEIMIA, BIBERSTEINIA

  1. Habitat
  2. Morphology
  3. Staining
  4. Culture
A 
1. Habitat:
• mucous membranes (resp.)
2. Morphology
• 0.5-2 µm coccoid rods
3. Staining:
• Gram negative
• bipolar staining
4. Culture:
• fastidious
• yeast extract, blood, pH-susceptibility
• β hemolysis
52
Q

ACTINOBACILLUS

1. Habitat

A

alimentary tract, respiratory and genital ways, mucous membrane

53
Q

A. lignieresii

  1. Habitat
  2. Morphology
  3. Staining
  4. Culture
A
  1. Habitat: cattle, sheep oral cavity, rumen
  2. Morphology: 1-1.2 µm rods, filaments, sulphur granules in lesions (like: actinomycosis)
  3. Staining: Gram negative, bipolar
  4. Culture:
    • aerobic, facultative anaerobic,
    • poor growth on nutrient agar,
    • sticks to the agar
54
Q

A. equuli

  1. Habitat
  2. Morphology
  3. Culture
A
  1. Habitat: horse, swine alimentary tract, tonsils, respiratory tract
  2. Morphology: 1-2 µm rod, filaments
  3. Staining: Gram negative
  4. Culture:
    • large, viscous colonies
    • some strains can haemolyse
55
Q

A. suis

  1. Habitat
  2. Morphology/Staining
  3. Culture
A
  1. Habitat: swine mucous membranes (respiratory and genital tract)
  2. Morphology: Gram negative rods
  3. Culture: blood agar, β haemolysis
56
Q

A. pleuropneumoniae

  1. Morphology
  2. Culture
A 
A. pleuropneumoniae
1. Morphology:
• coccoid, short rods, some strains fimbria
2. Culture:
• fastidious,
• ß haemolysis
• blood agar, chocolate agar with yeast extract
• biotypes: NAD (V factor) demand
• biotype 1: V-factor demand
• satellitism, NAD, fresh yeast extract
• biotype 2: V-factor is not needed
57
Q

HAEMOPHILUS, AVIBACTERIUM

  1. Habitat
  2. Morphology
  3. Staining
A
  1. Habitat: mucous membranes of humans and animals (respiratory, genital)
  2. Morphology: 0.5-2 µm coccoid rods, filaments, (fimbria)
  3. Staining: Gram negative
58
Q

HISTOPHILUS

Species

A

Histophilus somni (Haemophilus somnus, Histophilus ovis, Haemophilus agni)

59
Q

HISTOPHILUS

  1. Habitat
  2. Morphology/Staining
  3. Culture
A
  1. Habitat: urogenital mucous membranes, respiratory way
  2. Morphology: Gram negative rods
  3. Culture:
    • fastidious
    • X, V factor not needed,
    • CO2-demand,
60
Q

OTHER GENERA HAVING SHARED
CHARACTERISTICS WITH THE FAMILY
PASTEURELLACEAE

A
  • Ornithobacterium
  • Riemerella
  • Taylorella
61
Q

Ornithobacterium rhinotracheale

  1. Habitat
  2. Morphology
  3. Staining
A
  1. Habitat: some bird species (turkey, chicken) upper respiratory ways
  2. Morphology: 1-3 µm rods
  3. Staining: Gram negative, bipolar
62
Q

Riemerella anatipestifer

  1. Habitat
  2. Morphology
  3. Staining
A
  1. Habitat: water fowl upper respiratory ways
  2. Morphology: 1-3 µm rods
  3. Staining: Gram negative, bipolar
63
Q

Taylorella (H.) equigenitalis

  1. Habitat
  2. Morphology
  3. Culture
A
  1. Habitat:
    • mucous membranes of the genitals of stallions,
    • clitoris of mares
  2. Morphology: Gram negative coccoid rods, filaments
  3. Culture:
    • fastidious
    • X and V factor not needed, CO2-demand,
64
Q

Identification of an unknown bacterium culture

A
  1. Inoculation on appropriate media
  2. Selection of suspected colonies, production of pure cultures
  3. Examination of cultural characteristics and colony morphology
  4. Primary tests
    • Gram staining, spores
    • Examination of movement
    • Catalase production
    • Oxidase production
    • Oxidative – fermentative test (glucose, aerobic/anaerobic prop.)
    • Identification of the genus or family
  5. Secondary tests
    • Examination of enzymes and products of the carbohydrate
    metabolism
    • Examination of enzymes and products of the nitrogen metabolism
    • Detection of extra cellular enzymes
  6. Identification of the bacterium
  7. Examination of antibiotic resistance
65
Q

PASTEURELLACEAE

5. Biochemistry

A
  • catalase +,
  • oxidase +,
  • fermentative
  • toxin production by some species
66
Q

PASTEURELLACEAE

  1. Antigens
  2. Resistance
  3. Pathogenicity
A
  1. Antigens: cell wall antigens, capsule antigens
  2. Resistance: weak
  3. Pathogenicity: facultative pathogens
67
Q

PASTEURELLA

Culture

A
  • fastidious:yeast extract, blood, pH-susceptibility
  • colony types: M-S-R colonies
  • smell
68
Q

PASTEURELLA

Biochemistry

A
  • catalase +, oxidase +,
  • fermentative
  • cytotoxin production: P. multocida D, (dermonecrotoxin)
  • P. multocida subspecies: biochemical differences
69
Q

PASTEURELLA

  1. Antigens
  2. Resistance
A 
6. Antigens
• P. multocida
• cell wall: 16 (Heddleston system), 11 (Namioka system)
• capsule: A, B, D, E, F
7. Resistance: weak
70
Q

PASTEURELLA

Pathogenicity

A

• P. multocida A, D: ruminants, swine pneumonia, rabbit
septicaemia, pneumonia, fowl cholera
• P. multocida D: atrophic rhinitis of swine,
• P. multocida B, E: haemorrhagic septicaemia
• P. pneumotropica: lab. rodents pneumonia, dog, cat oral cavity
• P. aerogenes: alimentary tract of swine, abortion
• P. canis, P. dagmatis, P. stomatis: dog, cat oral cavity, bite
• P. caballi: horse respiratory infection

71
Q

MANNHEIMIA, BIBERSTEINIA

Biochemistry

A
  • catalase +, oxidase +,
  • fermentative
  • leukotoxin production
72
Q

MANNHEIMIA, BIBERSTEINIA

  1. Antigens
  2. Resistance
A
  1. Antigens:
    • cell wall
    • capsule: M. haemolytica 12 types, B. trehalosi 4 types
  2. Resistance: weak
73
Q

MANNHEIMIA, BIBERSTEINIA

8. Pathogenicity

A
  • M. (P.) haemolytica (P. haemolytica A):
    •ruminants pneumonia,
    •lamb septicaemia,
    •mastitis,
    •arthritis.
  • B. trehalosi (P. haemolytica T, P. trehalosi): acute systemic pasteurellosis of feedlot lambs
74
Q

ACTINOBACILLUS

Species

A
  • A. lignieresii
  • A. equuli
  • A. suis
  • A. seminis
  • A. ureae
  • A. pleuropneumoniae
75
Q

A. lignieresii:

  1. Biochemistry
  2. Antigens
  3. Resistance
  4. Pathogenicity
A
  1. Biochemistry: catalase +, oxidase +, fermentative,
  2. Antigens: cell wall antigens, 6 serogroups
  3. Resistance: weak
  4. Pathogenicity: cattle, sheep, swine actinobacillosis
76
Q

A. equuli

  1. Resistance
  2. Subspecies, pathogenicity
A
  1. Resistance: weak
  2. Subspecies, pathogenicity:
    • A. equuli subsp. equuli
    • A. equuli subsp. haemolyticus
    both subspecies cause the same disease:
    • foal: septicaemia, nephritis, pneumonia, arthritis
    • horse: pyosepticaemia,
    • swine: septicaemia, arthritis
77
Q

A. suis

Pathogenicity

A
  • swine pneumonia, septicaemia, metritis

* suckling piglet: septicaemia

78
Q

A. seminis

  1. Habitat
  2. Culture
  3. Pathogenicity
A
  1. Habitat: mucous membranes of the genital tract of rams, semen
  2. Culture: 10% CO2 blood agar
  3. Pathogenicity: facultative pathogenic, inflammation of the accessory glands, epididymitis, abortion
79
Q

A. (Pasteurella) ureae:

  1. Habitat
  2. Pathogenicity
A
  1. Habitat: human respiratory way

2. Pathogenicity: human upper respiratory infection

80
Q

A. pleuropneumoniae

  1. Biochemistry
  2. Antigens
  3. Resistance
  4. Pathogenicity
A
  1. Biochemistry: Apx I-II-III-IV cytotoxin produced
  2. Antigens: 16 serogroups
  3. Resistance: weak
  4. Pathogenicity: swine haemorrhagic-necrotic pleuropneumonia
81
Q

HAEMOPHILUS, AVIBACTERIUM

  1. Biochemistry
  2. Antigens
  3. Resistance
  4. Pathogenicity
A
  1. Biochemistry: examination is difficult
  2. Antigens: cell wall antigens, capsule antigens
  3. Resistance: weak
  4. Pathogenicity: facultative pathogenic
82
Q

HAEMOPHILUS, AVIBACTERIUM

9. Species

A
  • H. influenzae: human upper respiratory ways, meningitis
  • H. parasuis: V factor demand, 15 serotypes, porcine polyserositis (Glässer’s disease)
  • Avibacterium (H.) paragallinarum:
    • CO2 and V factor demand,
    • 3 serogroups, 9 subgroups
    • infectious coryza of hens
83
Q

HISTOPHILUS

4. Pathogenicity

A
  • facultative pathogenic,
  • cattle:
    • septicaemia,
    • thromboembolic meningoencephalitis (TEME),
    • calf pneumonia,
    • abortion
    • mastitis
  • sheep:
    • epididymitis-orchitis,
    • abortion,
    • mastitis,
    • lamb septicaemia
84
Q

Ornithobacterium rhinotracheale

  1. Culture
  2. Biochemistry
  3. Resistance
  4. Pathogenicity
A
  1. Culture: fastidious, blood agar, 10% CO2
  2. Biochemistry: catalase -, oxidase +,
  3. Resistance: weak
  4. Pathogenicity:
    • facultative pathogenic,
    • turkey, chicken bronchitis
85
Q

Riemerella anatipestifer

  1. Culture
  2. Biochemistry
  3. Resistance
  4. Pathogenicity
A
  1. Culture
  2. Biochemistry
  3. Resistance
  4. Pathogenicity
86
Q

TAYLORELLA

Pathogenicity

A

CEM: contagious equine metritis

Bacteriology (14 decks)

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