Lab 6 - Enterobacterias

Question 1

Enterobacteria can be found in the gastrointestinal tract only

Answer 1

False (also in genital tract, respiratory tract and in the environment)

Question 2

Enterobacteria are middle-sized Gram-positive rods

Answer 2

False, gram negative

Question 3

Oedema-disease is caused by E. coli in neonatal piglets

Answer 3

False, in weaned piglets

Question 4

Enterobacteria are fastidious bacteria

Answer 4

False (simple)

Question 5

E. coli strains can form capsule which were isolated from swine

Answer 5

False, in calf (swine: haemolysis)

Question 6

Bismuth-sulphite agar is used to isolate E. coli

Answer 6

False, used to isolate salmonella

Question 7

Klebsiella pneumonia is flagellated

Answer 7

False, no flagella

Question 8

Enterobacteria are Gram-negative bacteria

Answer 8

True

Question 9

Klebsiella species are lactose-negative bacteria

Answer 9

False, they are lactose-positive

Question 10

Enterobacteria are obligate anaerobic bacteria

Answer 10

False, (they are aerobic, facultative anaerobic)

Question 11

Enterobacteria are oxidase-negative bacteria

Answer 11

True

Question 12

Shigella species are motile bacteria

Answer 12

False, not motile

Question 13

MacConkey agar contains phenolred as a pH-indicator

Answer 13

False, contains Drigalski agar (neutral red)

Question 14

MacConkey agar contains glucose as a carbon source

Answer 14

False, lactose

Question 15

Pathogenic E. coli strains isolated from the gut of piglets posses F5, F17 or F41 fimbrial antigens

Answer 15

False (F4, F6, F18=swine, F5, F17, F41 = cattle)

Question 16

E. coli can cause septicaemia in calves

Answer 16

True

Question 17

Klebsiella pneumonia cannot produce capsule

Answer 17

False, has capsule

Question 18

Enterobacteria cloacae are a lactose-negative bacterium

Answer 18

False, lactose positive

Question 19

E. coli is an obligate pathogenic species

Answer 19

False (most of them are saprophytic, some are facultative pathogenic)

Question 20

E. coli is an indole-positive bacterium

Answer 20

True

Question 21

E.coli strain in swine shows b-haemolysis

Answer 21

True?

Question 22

Coli diarrhea and edema is found in neonatal pigs

Answer 22

False (in weaned pigs, neonatal pigs have only coli diarrhea)

Question 23

MacConkey has lactose as a sugar source

Answer 23

True

Question 24

Urease is positive in both klebsiella and E. coli

Answer 24

False, only in Klebsiella

Question 25

E-coli is indol positive

Answer 25

True (not Klebsiella or Enterobacter)

Question 26

Enterobacteria and klebsiella can be differentiated with a catalase test and OF test

Answer 26

?

Question 27

F2 and F3 antigens are found in human strains of E. coli

Answer 27

True

Question 28

Enterobacteria are facultative pathogens

Answer 28

True

Question 29

Enterobacterias are motile bacterias

Answer 29

True (flagella)

Question 30

Chromocult Coloform Agar can differentiate coliform and E. coli

Answer 30

True

Question 31

Enterobacteria are members of normal gut flora

Answer 31

True

Question 32

As a result of gram staining, E coli is blue

Answer 32

True (gram neg.)

Question 33

Enterobacteria are obligate aerobic bacteria

Answer 33

False, Facultative aerobic

Question 34

Klebsiella pneumonia is a lactose-negative bacterium

Answer 34

False (lactose positive, same as E.coli and enterobacter)

Question 35

The sign of cell wall antigen is H

Answer 35

False (H is flagella, O is well call)

Question 36

E.coli strains can cause haemolysis on blood agar which were isolated

Answer 36

True? (from swine)

Question 37

Oedema disease is caused by E.coli in calf

Answer 37

False (piglets)

Question 38

E.coli can cause coli-granulomatosis in older poultry

Answer 38

True

Question 39

Klebsiella pneumonia has large, merging, mucoid colonies

Answer 39

True

Question 40

Pneumonia in human can be caused by Klebsiella pneumonia

Answer 40

True

Question 41

Enterobacteria cloacae is an urease-positive bacteria

Answer 41

False (negative)

Question 42

Enterobacteria morphology

Answer 42

* 1-3 µm rod, | * flagella (except: Shigella, Klebsiella)

Question 43

Which bacteria use Rambach agar?

Answer 43

Salmonella

Question 44

Enterobacteria biochemistry

Answer 44

* catalase +, * oxidase -, * fermentative (acid + gas)

Question 45

Enterobacteria antigens

Answer 45

* O: cell wall (LPS) * K: capsule (polysaccharide) * H: flagella (protein) * F: fimbria (protein)

Question 46

Enterobacteria resistance

Answer 46

medium

Question 47

Enterobacteria: pathogenetic characteristics

Answer 47

obligate pathogenic - facultative pathogenic - saprophytic

Question 48

Grouping of enterobacterias

Answer 48

1. genus: biochemical characteristics 2. species: biochemical and serological characteristics 3. serogroups: O antigens 4. serotype: O-K-H-F antigens 5. biotypes: fermentation pattern

Question 49

Genera of the family Enterobacteriaceae

Answer 49

``` Lactose-POS: Escherichia, Klebsiella, Enterobacter Lactose-NEG: •Salmonella •Shigella •Yersinia •Proteus •Providencia •Edwardsiella •Serratia •Citrobacter ```

Question 50

``` ESCHERICHIA E. coli 1. Habitat 2. Morphology 3. Staining ```

Answer 50

1. Habitat: gut, aerobic, facultative anaerobic gut flora 2. Morphology: 2-3 µm rod, capsule, flagella, fimbria 3. Staining: Gram negative

Question 51

ESCHERICHIA E. coli Culture

Answer 51

* as Enterobacteriaceae * capsule: E. coli strains from calf * haemolysis: E. coli strains from swine

Question 52

ESCHERICHIA E. coli Biochemistry

Answer 52

* lactose + | * indol +

Question 53

``` E.coli - Klebsiella - Enterobacter: positive or negative? 1. Indol 2. Urease 3. Lactose 4. Mannitol 5. Methyl red 6. Voges-Proskauer 7. H2S 8. Phenylalaninedeaminase ```

Answer 53

E.coli - Klebsiella - Enterobacter: 1. Indol: + / - / - 2. Urease: - / + / - 3. Lactose: + / + / + 4. Mannitol: + / + / + 5. Methyl red: + / - / - 6. Voges-Proskauer - / + / + 7. H2S: - / - / - 8. Phenylalaninedeaminase: - / - / -

Question 54

ESCHERICHIA E. coli Antigens

Answer 54

* O: type specific (187 types) * K: heat stabile (A) and heat labile (L): 80 types * F: 18 types * F1: common fimbria * F2, F3: human * F4 (K88), F6 (987p), F18 ab/ac: swine * F5 (K99), F17, F41: cattle * F7-F16: UTI (urinary tract infection): human, poultry * H: 60 types

Question 55

ESCHERICHIA E. coli Resistance

Answer 55

medium

Question 56

ESCHERICHIA E. coli Pathogenetic characteristics

Answer 56

saprophyte - facultative pathogenic

Question 57

ESCHERICHIA E. coli Pathogenicity - which species is affected and which symptoms?

Answer 57

CATTLE: •calf coli-diarrhoea (neonatal) •calf coli-septicaemia •cow: mastitis SWINE: •coli-diarrhoea of neonatal piglets •weaned piglets: coli-diarrhoea, oedema disease •sow: mastitis, metritis LAMB: coli-diarrhoea (neonatal), watery mouth POULTRY: •day-old-chicken: septicaemia •chicken: septicaemia, respiratory disease •older: lesions in organs (airsacculitis, peritonitis, coligranulomatosis) FOAL, DOG, CAT, MINK, FOX, NUTRIA, CHINCHILLA: • coli-diarrhoea, septicaemia HUMAN: • neonatal enteritis, septicaemia, • urogenital infections, • enteritis, • haemorrhagic enterocolitis, haemolytic uraemic syndrome

Question 58

Pathogenic E. coli strains

Answer 58

* enteropathogenic strains: intimin * enterotoxigenic strains: adhesive factors, enterotoxins * verotoxigenic strains * necrotoxic strains: CNF * enteroinvasive strains (human): like Shigella * enteroadhesive-aggregative strains (human) * septicaemic strains

Question 59

``` KLEBSIELLA K. pneumoniae 1. Habitat 2. Morphology 3. Culture ```

Answer 59

1. Habitat: gut, respiratory way, genital tract, environment 2. Morphology: rod, capsule, no flagellum 3. Culture: • large, merging, mucoid colonies • rapid change of the colour of the indicator

Question 60

KLEBSIELLA K. pneumoniae Biochemistry

Answer 60

* lactose + * Voges-Proskauer test + * urease +

Question 61

KLEBSIELLA K. pneumoniae Antigens

Answer 61

82 serogroups (capsule)

Question 62

KLEBSIELLA K. pneumoniae Pathogenetic characteristics

Answer 62

saprophytic - facultative pathogenic

Question 63

KLEBSIELLA K. pneumoniae Pathogenicity - which species is affected and which symptoms?

Answer 63

* calf: bronchitis, bronchopneumonia * cow: mastitis * mare: metritis * human: pneumonia, urogenital infections

Question 64

ENTEROBACTER 1. Habitat 2. Morphology 3. Culture

Answer 64

1. Habitat: gut, environment 2. Morphology: rod, capsule 3. Culture: mucoid colonies

Question 65

ENTEROBACTER | Biochemistry

Answer 65

* lactose + | * Voges-Proskauer test +

Question 66

ENTEROBACTER | Pathogenetic characteristics

Answer 66

* saprophytic | * rarely respiratory infection in humans

Question 67

ENTEROBACTER | Species

Answer 67

* E. cloacae, | * E. aerogenes