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Bacteriology > Lab 6 - Enterobacterias > Flashcards

Lab 6 - Enterobacterias Flashcards

1
Q

Enterobacteria can be found in the gastrointestinal tract only

A

False (also in genital tract, respiratory tract and in the environment)

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2
Q

Enterobacteria are middle-sized Gram-positive rods

A

False, gram negative

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3
Q

Oedema-disease is caused by E. coli in neonatal piglets

A

False, in weaned piglets

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4
Q

Enterobacteria are fastidious bacteria

A

False (simple)

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5
Q

E. coli strains can form capsule which were isolated from swine

A

False, in calf (swine: haemolysis)

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6
Q

Bismuth-sulphite agar is used to isolate E. coli

A

False, used to isolate salmonella

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7
Q

Klebsiella pneumonia is flagellated

A

False, no flagella

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8
Q

Enterobacteria are Gram-negative bacteria

A

True

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9
Q

Klebsiella species are lactose-negative bacteria

A

False, they are lactose-positive

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10
Q

Enterobacteria are obligate anaerobic bacteria

A

False, (they are aerobic, facultative anaerobic)

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11
Q

Enterobacteria are oxidase-negative bacteria

A

True

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12
Q

Shigella species are motile bacteria

A

False, not motile

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13
Q

MacConkey agar contains phenolred as a pH-indicator

A

False, contains Drigalski agar (neutral red)

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14
Q

MacConkey agar contains glucose as a carbon source

A

False, lactose

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15
Q

Pathogenic E. coli strains isolated from the gut of piglets posses F5, F17 or F41 fimbrial antigens

A

False (F4, F6, F18=swine, F5, F17, F41 = cattle)

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16
Q

E. coli can cause septicaemia in calves

A

True

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17
Q

Klebsiella pneumonia cannot produce capsule

A

False, has capsule

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18
Q

Enterobacteria cloacae are a lactose-negative bacterium

A

False, lactose positive

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19
Q

E. coli is an obligate pathogenic species

A

False (most of them are saprophytic, some are facultative pathogenic)

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20
Q

E. coli is an indole-positive bacterium

A

True

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21
Q

E.coli strain in swine shows b-haemolysis

A

True?

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22
Q

Coli diarrhea and edema is found in neonatal pigs

A

False (in weaned pigs, neonatal pigs have only coli diarrhea)

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23
Q

MacConkey has lactose as a sugar source

A

True

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24
Q

Urease is positive in both klebsiella and E. coli

A

False, only in Klebsiella

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25
Q

E-coli is indol positive

A

True (not Klebsiella or Enterobacter)

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26
Q

Enterobacteria and klebsiella can be differentiated with a catalase test and OF test

A

?

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27
Q

F2 and F3 antigens are found in human strains of E. coli

A

True

28
Q

Enterobacteria are facultative pathogens

A

True

29
Q

Enterobacterias are motile bacterias

A

True (flagella)

30
Q

Chromocult Coloform Agar can differentiate coliform and E. coli

A

True

31
Q

Enterobacteria are members of normal gut flora

A

True

32
Q

As a result of gram staining, E coli is blue

A

True (gram neg.)

33
Q

Enterobacteria are obligate aerobic bacteria

A

False, Facultative aerobic

34
Q

Klebsiella pneumonia is a lactose-negative bacterium

A

False (lactose positive, same as E.coli and enterobacter)

35
Q

The sign of cell wall antigen is H

A

False (H is flagella, O is well call)

36
Q

E.coli strains can cause haemolysis on blood agar which were isolated

A

True? (from swine)

37
Q

Oedema disease is caused by E.coli in calf

A

False (piglets)

38
Q

E.coli can cause coli-granulomatosis in older poultry

A

True

39
Q

Klebsiella pneumonia has large, merging, mucoid colonies

A

True

40
Q

Pneumonia in human can be caused by Klebsiella pneumonia

A

True

41
Q

Enterobacteria cloacae is an urease-positive bacteria

A

False (negative)

42
Q

Enterobacteria morphology

A
  • 1-3 µm rod,

* flagella (except: Shigella, Klebsiella)

43
Q

Which bacteria use Rambach agar?

A

Salmonella

44
Q

Enterobacteria biochemistry

A
  • catalase +,
  • oxidase -,
  • fermentative (acid + gas)
45
Q

Enterobacteria antigens

A
  • O: cell wall (LPS)
  • K: capsule (polysaccharide)
  • H: flagella (protein)
  • F: fimbria (protein)
46
Q

Enterobacteria resistance

A

medium

47
Q

Enterobacteria: pathogenetic characteristics

A

obligate pathogenic - facultative pathogenic - saprophytic

48
Q

Grouping of enterobacterias

A
  1. genus: biochemical characteristics
  2. species: biochemical and serological characteristics
  3. serogroups: O antigens
  4. serotype: O-K-H-F antigens
  5. biotypes: fermentation pattern
49
Q

Genera of the family Enterobacteriaceae

A 
Lactose-POS: Escherichia, Klebsiella, Enterobacter
Lactose-NEG:
•Salmonella
•Shigella
•Yersinia
•Proteus
•Providencia
•Edwardsiella
•Serratia
•Citrobacter
50
Q 
ESCHERICHIA
E. coli
1. Habitat
2. Morphology
3. Staining
A
  1. Habitat: gut, aerobic, facultative anaerobic gut flora
  2. Morphology: 2-3 µm rod, capsule, flagella, fimbria
  3. Staining: Gram negative
51
Q

ESCHERICHIA
E. coli
Culture

A
  • as Enterobacteriaceae
  • capsule: E. coli strains from calf
  • haemolysis: E. coli strains from swine
52
Q

ESCHERICHIA
E. coli
Biochemistry

A
  • lactose +

* indol +

53
Q 
E.coli - Klebsiella - Enterobacter:
positive or negative?
1. Indol
2. Urease
3. Lactose
4. Mannitol
5. Methyl red
6. Voges-Proskauer
7. H2S
8. Phenylalaninedeaminase
A

E.coli - Klebsiella - Enterobacter:

  1. Indol: + / - / -
  2. Urease: - / + / -
  3. Lactose: + / + / +
  4. Mannitol: + / + / +
  5. Methyl red: + / - / -
  6. Voges-Proskauer - / + / +
  7. H2S: - / - / -
  8. Phenylalaninedeaminase: - / - / -
54
Q

ESCHERICHIA
E. coli
Antigens

A
  • O: type specific (187 types)
  • K: heat stabile (A) and heat labile (L): 80 types
  • F: 18 types
  • F1: common fimbria
  • F2, F3: human
  • F4 (K88), F6 (987p), F18 ab/ac: swine
  • F5 (K99), F17, F41: cattle
  • F7-F16: UTI (urinary tract infection): human, poultry
  • H: 60 types
55
Q

ESCHERICHIA
E. coli
Resistance

A

medium

56
Q

ESCHERICHIA
E. coli
Pathogenetic characteristics

A

saprophyte - facultative pathogenic

57
Q

ESCHERICHIA
E. coli
Pathogenicity
- which species is affected and which symptoms?

A

CATTLE:
•calf coli-diarrhoea (neonatal)
•calf coli-septicaemia
•cow: mastitis
SWINE:
•coli-diarrhoea of neonatal piglets
•weaned piglets: coli-diarrhoea, oedema disease
•sow: mastitis, metritis
LAMB: coli-diarrhoea (neonatal), watery mouth
POULTRY:
•day-old-chicken: septicaemia
•chicken: septicaemia, respiratory disease
•older: lesions in organs (airsacculitis, peritonitis, coligranulomatosis)
FOAL, DOG, CAT, MINK, FOX, NUTRIA, CHINCHILLA:
• coli-diarrhoea, septicaemia
HUMAN:
• neonatal enteritis, septicaemia,
• urogenital infections,
• enteritis,
• haemorrhagic enterocolitis, haemolytic uraemic syndrome

58
Q

Pathogenic E. coli strains

A
  • enteropathogenic strains: intimin
  • enterotoxigenic strains: adhesive factors, enterotoxins
  • verotoxigenic strains
  • necrotoxic strains: CNF
  • enteroinvasive strains (human): like Shigella
  • enteroadhesive-aggregative strains (human)
  • septicaemic strains
59
Q 
KLEBSIELLA
K. pneumoniae
1. Habitat
2. Morphology
3. Culture
A
  1. Habitat: gut, respiratory way, genital tract, environment
  2. Morphology: rod, capsule, no flagellum
  3. Culture:
    • large, merging, mucoid colonies
    • rapid change of the colour of the indicator
60
Q

KLEBSIELLA
K. pneumoniae
Biochemistry

A
  • lactose +
  • Voges-Proskauer test +
  • urease +
61
Q

KLEBSIELLA
K. pneumoniae
Antigens

A

82 serogroups (capsule)

62
Q

KLEBSIELLA
K. pneumoniae
Pathogenetic characteristics

A

saprophytic - facultative pathogenic

63
Q

KLEBSIELLA
K. pneumoniae
Pathogenicity
- which species is affected and which symptoms?

A
  • calf: bronchitis, bronchopneumonia
  • cow: mastitis
  • mare: metritis
  • human: pneumonia, urogenital infections
64
Q

ENTEROBACTER

  1. Habitat
  2. Morphology
  3. Culture
A
  1. Habitat: gut, environment
  2. Morphology: rod, capsule
  3. Culture: mucoid colonies
65
Q

ENTEROBACTER

Biochemistry

A
  • lactose +

* Voges-Proskauer test +

66
Q

ENTEROBACTER

Pathogenetic characteristics

A
  • saprophytic

* rarely respiratory infection in humans

67
Q

ENTEROBACTER

Species

A
  • E. cloacae,

* E. aerogenes

Bacteriology (14 decks)

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